Abstract
Serial microscopic immunodiffusion assays of F cells, i.e., erythrocytes that contain fetal hemoglobin (HbF), in four individuals recovering from anemia demonstrate initial increases in the percentage of circulating reticulocytes that contain HbF (F reticulocytes) and subsequent increases in the percentage of mature erythrocytes that contain HbF (F erythrocytes). In one individual responding to therapy for iron-deficiency anemia, the average percentage of F reticulocytes increased from 4.8±1.1 to 16.0±2.8% (mean±SD), while the mean level of F erythrocytes increased from 3.5±0.7 to 7.2±0.6%. Two normal children with transient erythroblastopenia exhibited F reticulocyte percentages of 71.3±6.7 and 41.5±1.5%, respectively, when erythropoiesis resumed. With recovery these values fell to finally measured values of 33.7±4.7 and 12.6±1.1%, respectively. In an adolescent with sickle cell anemia, F-reticulocyte percentages fluctuated between 0.6±1.1 and 34.0±2.8% and paralleled the rise and fall of total reticulocytes associated with therapy for a nasopharyngeal carcinoma.
Such findings suggest that first, the production of F cells and non-F cells are separately regulated. Second, F-cell production is preferentially stimulated during escape from erythropoietic suppression and selectively depressed at the start of suppression. Third, during escape from erythropoietic suppression, F-cell production in vivo resembles that reported for in vitro cultures of erythroid stem cells. Fourth, individuals with sickle cell anemia, like individuals without hemoglobinopathies, can change their relative level of F-cell production.
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- Alter B. P., Rappeport J. M., Huisman T. H., Schroeder W. A., Nathan D. G. Fetal erythropoiesis following bone marrow transplantation. Blood. 1976 Dec;48(6):843–853. [PubMed] [Google Scholar]
- Bertles J. F. Human fetal hemoglobin: significance in disease. Ann N Y Acad Sci. 1974 Nov 29;241(0):638–652. doi: 10.1111/j.1749-6632.1974.tb21920.x. [DOI] [PubMed] [Google Scholar]
- Boyer S. H., Margolet L., Boyer M. L., Huisman T. H., Schroeder W. A., Wood W. G., Weatherall D. J., Clegg J. B., Cartner R. Inheritance of F cell frequency in heterocellular hereditary persistence of fetal hemoglobin: an example of allelic exclusion. Am J Hum Genet. 1977 May;29(3):256–271. [PMC free article] [PubMed] [Google Scholar]
- Dover G. J., Boyer S. H., Bell W. R. Microscopic method for assaying F cell production: illustrative changes during infancy and in aplastic anemia. Blood. 1978 Oct;52(4):664–672. [PubMed] [Google Scholar]
- Dover G. J., Boyer S. H., Charache S., Heintzelman K. Individual variation in the production and survival of F cells in sickle-cell disease. N Engl J Med. 1978 Dec 28;299(26):1428–1435. doi: 10.1056/NEJM197812282992603. [DOI] [PubMed] [Google Scholar]
- Papayannopoulou T., Brice M., Stamatoyannopoulos G. Hemoglobin F synthesis in vitro: evidence for control at the level of primitive erythroid stem cells. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2923–2927. doi: 10.1073/pnas.74.7.2923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sheridan B. L., Weatherall D. J., Clegg J. B., Pritchard J., Wood W. G., Callender S. T., Durrant I. J., McWhirter W. R., Ali M., Partridge J. W. The patterns of fetal haemoglobin production in leukaemia. Br J Haematol. 1976 Apr;32(4):487–506. doi: 10.1111/j.1365-2141.1976.tb00952.x. [DOI] [PubMed] [Google Scholar]