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. 1979 May;63(5):1042–1049. doi: 10.1172/JCI109373

Taurocholate pool size and distribution in the fetal rat.

J M Little, J E Richey, D H Van Thiel, R Lester
PMCID: PMC372047  PMID: 447826

Abstract

Taurocholate concentrations in fetal and neonatal rats were determined by radioimmunoassay. Total body taurocholate pool size varied from 0.0049 +/- 0.0008 to 203 +/- 8 nmol/g body weight from day 5 of gestation to 5 d after birth. A 50-fold increase in taurocholate pool size was observed between days 15 and 19 of gestation. The distribution of taurocholate between liver, intestine, and the remainder of the carcass was determined for rats of gestational age 19 d to 5 d after birth. The major fraction of total body taurocholate was in the liver and intestine, with less than 15% in the remainder of the carcass. The ratio of taurocholate in intestine to taurocholate in liver, which was 1:17 at 19 d of gestation, had altered substantially to a ratio of 6:1 by 5 d after birth. Treatment of pregnant rats with 60 microgram/d of dexamethasone from gestational day 9 until sacrifice increased fetal taurocholate pool size by 80% at 15 d, 40% at 19 d, and 16% at 1 d after birth. Administration of dexamethasone to the mother also changed the ratio of taurocholate in intestine to taurocholate in liver. At 19 d of gestation, dexamethasone-treated mothers had fetuses with approximately equal amounts of taurocholate in intestine and liver. This suggested that adrenocorticosteroids stimulate the early maturation of factors controlling taurocholate pool size and tissue distribution in the rat fetus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BONGIOVANNI A. M. BILE ACID CONTENT OF GALLBLADDER OF INFANTS, CHILDREN AND ADULTS. J Clin Endocrinol Metab. 1965 May;25:678–685. doi: 10.1210/jcem-25-5-678. [DOI] [PubMed] [Google Scholar]
  2. Billat C., Plas C., Jacquot R. Etude en microscopie électronique d'hépatocytes faetaux de rat en culture primaire: action du cortisol. C R Acad Sci Hebd Seances Acad Sci D. 1972 Apr 17;274(16):2338–2340. [PubMed] [Google Scholar]
  3. Challacombe D. N., Edkins S., Brown G. A. Duodenal bile acids in infancy. Arch Dis Child. 1975 Nov;50(11):837–843. doi: 10.1136/adc.50.11.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chedid A., Nair V. Ontogenesis of cytoplasmic organelles in rat hepatocytes and the effects of prenatal phenobarbital on endoplasmic reticulum development. Dev Biol. 1974 Jul;39(1):49–62. doi: 10.1016/s0012-1606(74)80007-2. [DOI] [PubMed] [Google Scholar]
  5. Danielsson H., Rutter W. J. The metabolism of bile acids in the developing rat liver. Biochemistry. 1968 Jan;7(1):346–352. doi: 10.1021/bi00841a044. [DOI] [PubMed] [Google Scholar]
  6. De Wolf-Peeters C., De Vos R., Desmet V. Electron microscopy and histochemistry of canalicular differentiation in fetal and neonatal rat liver. Tissue Cell. 1972;4(3):379–388. doi: 10.1016/s0040-8166(72)80016-8. [DOI] [PubMed] [Google Scholar]
  7. Demers L. M., Hepner G. Radioimmunoassay of bile acids in serum. Clin Chem. 1976 May;22(5):602–606. [PubMed] [Google Scholar]
  8. ENCRANTZ J. C., SJOVALL J. On the bile acids in duodenal contents of infants and children. Bile acids and steroids 72. Clin Chim Acta. 1959 Nov;4:793–799. doi: 10.1016/0009-8981(59)90030-0. [DOI] [PubMed] [Google Scholar]
  9. ERLANGER B. F., BOREK F., BEISER S. M., LIEBERMAN S. Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone. J Biol Chem. 1959 May;234(5):1090–1094. [PubMed] [Google Scholar]
  10. Eyssen H., Parmentier G., Compernolle F., Boon J., Eggermont E. Trihydroxycoprostanic acid in the duodenal fluid of two children with intrahepatic bile duct anomalies. Biochim Biophys Acta. 1972 Jun 26;273(1):212–221. doi: 10.1016/0304-4165(72)90209-7. [DOI] [PubMed] [Google Scholar]
  11. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  12. Hanson R. F., Isenberg J. N., Williams G. C., Hachey D., Szczepanik P., Klein P. D., Sharp H. L. The metabolism of 3alpha, 7alpha, 12alpha-trihydorxy-5beta-cholestan-26-oic acid in two siblings with cholestasis due to intrahepatic bile duct anomalies. An apparent inborn error of cholic acid synthesis. J Clin Invest. 1975 Sep;56(3):577–587. doi: 10.1172/JCI108127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hofmann A. F., Small D. M. Detergent properties of bile salts: correlation with physiological function. Annu Rev Med. 1967;18:333–376. doi: 10.1146/annurev.me.18.020167.002001. [DOI] [PubMed] [Google Scholar]
  14. Jackson B. T., Smallwood R. A., Piasecki G. J., Brown A. S., Rauschecker H. F., Lester R. Fetal bile salt metabolism. I. The metabolism of sodium cholate-14C in the fetal dog. J Clin Invest. 1971 Jun;50(6):1286–1294. doi: 10.1172/JCI106607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lambiotte M., Vorbrodt A., Benedetti E. L. Expression of differentiation of rat foetal hepatocytes in cellular culture under the action of glucocorticoids: appearance of bile canaliculi. Cell Differ. 1973 Mar;2(1):43–53. doi: 10.1016/0045-6039(73)90005-5. [DOI] [PubMed] [Google Scholar]
  16. Lester R., Smallwood R. A., Little J. M., Brown A. S., Piasecki G. J., Jackson B. T. Fetal bile salt metabolism. The intestinal absorption of bile salt. J Clin Invest. 1977 Jun;59(6):1009–1016. doi: 10.1172/JCI108723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Little J. M., Smallwood R. A., Lester R., Piasecki G. J., Jackson B. T. Bile-salt metabolism in the primate fetus. Gastroenterology. 1975 Dec;69(6):1315–1320. [PubMed] [Google Scholar]
  18. Makino I., Sjövall J., Norman A., Strandvik B. Excretion of 3beta-hydroxy-5-cholenoic and 3a-hydroxy-5a-cholanoic acids in urine of infants with biliary atresia. FEBS Lett. 1971 Jun 10;15(2):161–164. doi: 10.1016/0014-5793(71)80047-9. [DOI] [PubMed] [Google Scholar]
  19. Norman A., Strandvik B., Ojamäe O. Bile acids and pancreatic enzymes during absorption in the newborn. Acta Paediatr Scand. 1972 Sep;61(5):571–576. doi: 10.1111/j.1651-2227.1972.tb15947.x. [DOI] [PubMed] [Google Scholar]
  20. POLEY J. R., DOWER J. C., OWEN C. A., Jr, STICKLER G. B. BILE ACIDS IN INFANTS AND CHILDREN. J Lab Clin Med. 1964 May;63:838–846. [PubMed] [Google Scholar]
  21. Palmer R. H., Bolt M. G. Bile acid sulfates. I. Synthesis of lithocholic acid sulfates and their identification in human bile. J Lipid Res. 1971 Nov;12(6):671–679. [PubMed] [Google Scholar]
  22. Peric-Golia L., Socic H. Biliary bile acids and cholesterol in developing sheep. Am J Physiol. 1968 Nov;215(5):1284–1287. doi: 10.1152/ajplegacy.1968.215.5.1284. [DOI] [PubMed] [Google Scholar]
  23. Rodbard D., Bridson W., Rayford P. L. Rapid calculation of radioimmunoassay results. J Lab Clin Med. 1969 Nov;74(5):770–781. [PubMed] [Google Scholar]
  24. Roovers J., Evrard E., Vanderhaeghe H. An improved method for measuring human blood bile acids. Clin Chim Acta. 1968 Mar;19(3):449–457. doi: 10.1016/0009-8981(68)90272-6. [DOI] [PubMed] [Google Scholar]
  25. Roy C. C., Ste-Marie M., Chartrand L., Weber A., Bard H., Doray B. Correction of the malabsorption of the preterm infant with a medium-chain triglyceride formula. J Pediatr. 1975 Mar;86(3):446–450. doi: 10.1016/s0022-3476(75)80983-8. [DOI] [PubMed] [Google Scholar]
  26. Sandberg D. H. Bile acid concentrations in serum during infancy and childhood. Pediatr Res. 1970 May;4(3):262–267. doi: 10.1203/00006450-197005000-00005. [DOI] [PubMed] [Google Scholar]
  27. Signer E., Murphy G. M., Edkins S., Anderson C. M. Role of bile salts in fat malabsorption of premature infants. Arch Dis Child. 1974 Mar;49(3):174–180. doi: 10.1136/adc.49.3.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Simmonds W. J., Korman M. G., Go V. L., Hofmann A. F. Radioimmunoassay of conjugated cholyl bile acids in serum. Gastroenterology. 1973 Nov;65(5):705–711. [PubMed] [Google Scholar]
  29. Smallwood R. A., Jablonski P., Watts J. M. Bile acid synthesis in the developing sheep liver. Clin Sci Mol Med. 1973 Sep;45(3):403–406. doi: 10.1042/cs0450403. [DOI] [PubMed] [Google Scholar]
  30. Smallwood R. A., Lester R., Plasecki G. J., Klein P. D., Greco R., Jackson B. T. Fetal bile salt metabolism. II. Hepatic excretion of endogenous bile salt and of a taurocholate load. J Clin Invest. 1972 Jun;51(6):1388–1397. doi: 10.1172/JCI106934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Subbiah M. T., Kuksis A., Mookerjea S. Secretion of bile salts by intact and isolated rat livers. Can J Biochem. 1969 Sep;47(9):847–854. doi: 10.1139/o69-133. [DOI] [PubMed] [Google Scholar]
  32. Subbiah M. T., Marai L., Dinh D. M., Penner J. W. Sterol and bile acid metabolism during development. 1. Studies on the gallbladder and intestinal bile acids of newborn and fetal rabbit. Steroids. 1977 Jan;29(1):83–92. doi: 10.1016/0039-128x(77)90111-8. [DOI] [PubMed] [Google Scholar]
  33. Tserng K. Y., Klein P. D. Synthesis of sulfate esters of lithocholic acid, glycolithocholic acid, and taurolithocholic acid with sulfur trioxide-triethylamine. J Lipid Res. 1977 Jul;18(4):491–495. [PubMed] [Google Scholar]
  34. WOOD R. L. AN ELECTRON MICROSCOPE STUDY OF DEVELOPING BILE CANALICULI IN THE RAT. Anat Rec. 1965 Apr;151:507–529. doi: 10.1002/ar.1091510403. [DOI] [PubMed] [Google Scholar]
  35. Watkins J. B., Ingall D., Szczepanik P., Klein P. D., Lester R. Bile-salt metabolism in the newborn. Measurement of pool size and synthesis by stable isotope technic. N Engl J Med. 1973 Mar 1;288(9):431–434. doi: 10.1056/NEJM197303012880902. [DOI] [PubMed] [Google Scholar]
  36. Watkins J. B., Szczepanik P., Gould J. B., Klein P., Lester R. Bile salt metabolism in the human premature infant. Preliminary observations of pool size and synthesis rate following prenatal administration of dexamethasone and phenobarbital. Gastroenterology. 1975 Sep;69(3):706–713. [PubMed] [Google Scholar]

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