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. 1977 Jun;59(6):1176–1187. doi: 10.1172/JCI108742

Modulatory effects on immunoglobulin synthesis and secretion by lymphocytes from immunodeficient patients.

S A Schwartz, Y S Choi, L Shou, R A Good
PMCID: PMC372331  PMID: 301145

Abstract

Incubation of peripheral lymphocytes (PBL) from normal donors with pokeweek mitogen (PWM) induced terminal differentiation by B lymphocytes to immunoglobulin (Ig) synthesizing and secreting plasma cells. B cells from hypogammaglobulinemic patients with different primary immunodeficiencies failed to undergo functional differentiation after similar treatment with PWM. Co-cultures of BL from normal donors and hypogammaglobulinemic patients often resulted in deviations, both positive and negative, from expected levels of PWM-stimulated intracellular Ig biosynthesis. Suppression of B-cell differentiation was manifested by PBL from patients with several different primary immunodeficiencies, including infantile sex-linked agammaglobulinemia. Immunoregulatory activities were noted to vary with the normal donor used in co-culture experiments and with time. Cell populations that were active in influencing B-cell differentiation to functional plasma cells did not have an appreciable modulatory effect on T-lymphocyte responses to mitogens. These observations may provide a functional subclassifications for immunoregulatory cells in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUTON O. C. Agammaglobulinemia. Pediatrics. 1952 Jun;9(6):722–728. [PubMed] [Google Scholar]
  2. Blaese R. M., Weiden P. L., Koski I., Dooley N. Infectious agammaglobulinemia: transmission of immunodeficiency with grafts of agammaglobulinemic cells. J Exp Med. 1974 Oct 1;140(4):1097–1101. doi: 10.1084/jem.140.4.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  4. Broom B. C., De la Concha E. G., Webster A. D., Janossy G. J., Asherson G. L. Intracellular immunoglobulin production in vitro by lymphocytes from patients with hypogammaglobulinaemia and their effect on normal lymphocytes. Clin Exp Immunol. 1976 Jan;23(1):73–77. [PMC free article] [PubMed] [Google Scholar]
  5. Buckley R. H., Whisnant K. J., Schiff R. I., Gilbertsen R. B., Huang A. T., Platt M. S. Correction of severe combined immunodeficiency by fetal liver cells. N Engl J Med. 1976 May 13;294(20):1076–1081. doi: 10.1056/NEJM197605132942002. [DOI] [PubMed] [Google Scholar]
  6. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  7. Choi Y. S., Good R. A. Development of chicken lymphoid system. I. Synthesis and secretion of immunoglobulins by chicken lymphoid cells. J Exp Med. 1972 May 1;135(5):1133–1150. doi: 10.1084/jem.135.5.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Choi Y. S. Serological precipitation method for studying biosynthesis and secretion of immunoglobulins by human peripheral blood lymphocytes. J Immunol Methods. 1977;14(1):37–40. doi: 10.1016/s0022-1759(97)90019-3. [DOI] [PubMed] [Google Scholar]
  9. Cooper M. D., Faulk W. P., Fudenberg H. H., Good R. A., Hitzig W., Kunkel H., Rosen F. S., Seligmann M., Soothill J., Wedgwood R. J. Classification of primary immunodeficiencies. N Engl J Med. 1973 May 3;288(18):966–967. doi: 10.1056/NEJM197305032881814. [DOI] [PubMed] [Google Scholar]
  10. Cooper M. D., Lawton A. R., Bockman D. E. Agammaglobulinaemia with B lymphocytes. Specific defect of plasma-cell differentiation. Lancet. 1971 Oct 9;2(7728):791–794. doi: 10.1016/s0140-6736(71)92742-5. [DOI] [PubMed] [Google Scholar]
  11. Eardley D. D., Gershon R. K. Induction of specific suppressor T cells in vitro. J Immunol. 1976 Jul;117(1):313–318. [PubMed] [Google Scholar]
  12. Eardley D. D., Staskawicz M. O., Gershon R. K. Suppressor cells: dependence on assay conditions for functional activity. J Exp Med. 1976 May 1;143(5):1211–1219. doi: 10.1084/jem.143.5.1211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fudenberg H., Good R. A., Goodman H. C., Hitzig W., Kunkel H. G., Roitt I. M., Rosen F. S., Rowe D. S., Seligmann M., Soothill J. R. Primary immunodeficiencies. Report of a World Health Organization Committee. Pediatrics. 1971 May;47(5):927–946. [PubMed] [Google Scholar]
  14. GOOD R. A., VARCO R. L., AUST J. B., ZAK S. J. Transplantation studies in patients with agammaglobulinemia. Ann N Y Acad Sci. 1957 Mar 22;64(5):882-924; discussion, 924-8. doi: 10.1111/j.1749-6632.1957.tb52482.x. [DOI] [PubMed] [Google Scholar]
  15. Geha R. S. Is the B-cell abnormality secondary to T-cell abnormality in severe combined immunodeficiency?1. Clin Immunol Immunopathol. 1976 Jul;6(1):102–106. doi: 10.1016/0090-1229(76)90065-9. [DOI] [PubMed] [Google Scholar]
  16. Geha R. S., Schneeberger E., Merler E., Rosen F. S. Heterogeneity of "acquired" or common variable agammaglobulinemia. N Engl J Med. 1974 Jul 4;291(1):1–6. doi: 10.1056/NEJM197407042910101. [DOI] [PubMed] [Google Scholar]
  17. Grey H. M., Rabellino E., Pirofsky B. Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphatic leukemia. J Clin Invest. 1971 Nov;50(11):2368–2375. doi: 10.1172/JCI106735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shou L., Schwartz S. A., Good R. A. Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors. J Exp Med. 1976 May 1;143(5):1100–1110. doi: 10.1084/jem.143.5.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siegal F. P., Siegal M., Good R. A. Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients. J Clin Invest. 1976 Jul;58(1):109–122. doi: 10.1172/JCI108439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stobo J. D., Paul S., Van Scoy R. E., Hermans P. E. Suppressor thymus-derived lymphocytes in fungal infection. J Clin Invest. 1976 Feb;57(2):319–328. doi: 10.1172/JCI108283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Twomey J. J., Laughter A. H., Farrow S., Douglass C. C. Hodgkin's disease. An immunodepleting and immunosuppressive disorder. J Clin Invest. 1975 Aug;56(2):467–475. doi: 10.1172/JCI108113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]
  23. Wu L. Y., Lawton A. R., Cooper M. D. Differentiation capacity of cultured B lymphocytes from immunodeficient patients. J Clin Invest. 1973 Dec;52(12):3180–3189. doi: 10.1172/JCI107518. [DOI] [PMC free article] [PubMed] [Google Scholar]

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