Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1977 Jul;60(1):1–6. doi: 10.1172/JCI108745

Selective release of archidonic acid from the phospholipids of human platelets in response to thrombin.

T K Bills, J B Smith, M J Silver
PMCID: PMC372337  PMID: 874074

Abstract

Arachidonic acid is unique amongst human platelet fatty acids in that it is the precursor of prostaglandins and thromboxanes. Since a number of these oxygenated products of arachidonic acid have potent effects on platelet function, an understanding of the metabolsim of their precursor is important. Human platelets have a mechanism for incorporating arachidonic acid from plasma into their phospholipids and, in response to thrombin, they reveal mechanisms for hydrolyzing this arachidonic acid from platelet phosphatidylcholine and phosphatidylinositol. This report deals with the specificity of these mechanisms. The present studies show that human platelets contain phospholipase A2 activities that preferentially release arachidonic acid. One of these activities specifically utilizes 1-acyl-2-arachidonyl-phosphatidyl-choline. Another utilizes platelet phosphatidylinositol and/or phosphatidylserine, both of which are highly enriched with arachidonic acid.

Full text

PDF
1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bills T. K., Smith J. B., Silver M. J. Metabolism of [14C]arachidonic acid by human platelets. Biochim Biophys Acta. 1976 Feb 23;424(2):303–314. doi: 10.1016/0005-2760(76)90198-3. [DOI] [PubMed] [Google Scholar]
  2. Cohen P., Derksen A. Comparison of phospholipid and fatty acid composition of human erythrocytes and platelets. Br J Haematol. 1969 Oct;17(4):359–371. doi: 10.1111/j.1365-2141.1969.tb01382.x. [DOI] [PubMed] [Google Scholar]
  3. DOLE V. P., JAMES A. T., WEBB J. P., RIZACK M. A., STURMAN M. F. The fatty acid patterns of plasma lipids during alimentary lipemia. J Clin Invest. 1959 Sep;38:1544–1554. doi: 10.1172/JCI103933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Danon A., Heimberg M., Oates J. A. Enrichment of rat tissue lipids with fatty acids that are prostaglandin precursors. Biochim Biophys Acta. 1975 Jun 23;388(3):318–330. doi: 10.1016/0005-2760(75)90090-9. [DOI] [PubMed] [Google Scholar]
  5. Hamberg M., Svensson J., Samuelsson B. Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2994–2998. doi: 10.1073/pnas.72.8.2994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamberg M., Svensson J., Wakabayashi T., Samuelsson B. Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation. Proc Natl Acad Sci U S A. 1974 Feb;71(2):345–349. doi: 10.1073/pnas.71.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iacono J. M., Zellner D. C., Paoletti R., Ishikawa T., Frigeni V., Fumagalli R. Comparison of blood platelet and erythrocyte lipids in man in three age groups from three regions: Milan, Cincinnati and Sicily. Haemostasis. 1973;2(1):141–162. doi: 10.1159/000214015. [DOI] [PubMed] [Google Scholar]
  8. Marcus A. J., Ullman H. L., Safier L. B. Lipid composition of subcellular particles of human blood platelets. J Lipid Res. 1969 Jan;10(1):108–114. [PubMed] [Google Scholar]
  9. Pace-Asciak C., Wolfe L. S. Inhibition of prostaglandin synthesis by oleic, linoleic and linolenic acids. Biochim Biophys Acta. 1968 Jul 1;152(4):784–787. doi: 10.1016/0005-2760(68)90126-4. [DOI] [PubMed] [Google Scholar]
  10. Smith J. B., Silver M. J., Ingerman C. M., Kocsis J. J. Prostaglandin D2 inhibits the aggregation of human platelets. Thromb Res. 1974 Sep;5(3):291–299. doi: 10.1016/0049-3848(74)90168-6. [DOI] [PubMed] [Google Scholar]
  11. Waite M., Sisson P. Partial purification and characterization of the phospholipase A 2 from rat liver mitochondria. Biochemistry. 1971 Jun 8;10(12):2377–2383. doi: 10.1021/bi00788a031. [DOI] [PubMed] [Google Scholar]
  12. Willis A. L., Comai K., Kuhn D. C., Paulsrud J. Dihomo-gamma-linolenate suppresses platelet aggregation when administered in vitro or in vivo. Prostaglandins. 1974 Dec 25;8(6):509–519. doi: 10.1016/0090-6980(74)90063-x. [DOI] [PubMed] [Google Scholar]
  13. Willis A. L., Vane F. M., Kuhn D. C., Scott C. G., Petrin M. An endoperoxide aggregator (Lass), formed in platelets in response to thrombotic stimuli: purification, identification and unique biological significance. Prostaglandins. 1974 Dec 25;8(6):453–507. doi: 10.1016/0090-6980(74)90062-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES