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. 2013 Jul 20;13:57. doi: 10.1186/1472-6750-13-57

Retraction: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

Ondrej Kaplan 1, Karel Bezouska 1,2, Ondrej Plihal 1, Rudiger Ettrich 3, Natallia Kulik 3, Ondrej Vanek 1,2, Daniel Kavan 1,2, Oldrich Benada 1, Anna Malandra 1,4, Ondrej Sveda 1, Alicja B Vesela 1, Anna Rinagelova 1, Kristyna Slamova 1, Maria Cantarella 4, Jurgen Felsberg 1, Jarmila Duskova 5, Jan Dohnalek 5, Michael Kotik 1, Vladimir Kren 1, Ludmila Martinkova 1,✉
PMCID: PMC3723435  PMID: 23870008

Retraction

The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.

Contributor Information

Ondrej Kaplan, Email: ondrej.kaplan@gmail.com.

Karel Bezouska, Email: bezouska@biomed.cas.cz.

Ondrej Plihal, Email: plihal@biomed.cas.cz.

Rudiger Ettrich, Email: ettrich@nh.usbe.cas.cz.

Natallia Kulik, Email: kulik@nh.usbe.cas.cz.

Ondrej Vanek, Email: kenav3@seznam.cz.

Daniel Kavan, Email: kuan@natur.cuni.cz.

Oldrich Benada, Email: benada@biomed.cas.cz.

Anna Malandra, Email: annamalandra@hotmail.it.

Ondrej Sveda, Email: ondrejsveda@seznam.cz.

Alicja B Vesela, Email: vesela@biomed.cas.cz.

Anna Rinagelova, Email: Anna.Rinagelova@vscht.cz.

Kristyna Slamova, Email: slamova.kristyna@gmail.com.

Maria Cantarella, Email: maria.cantarella@univaq.it.

Jurgen Felsberg, Email: felsberg@biomed.cas.cz.

Jarmila Duskova, Email: duskova@imc.cas.cz.

Jan Dohnalek, Email: dohnalek@imc.cas.cz.

Michael Kotik, Email: kotik@biomed.cas.cz.

Vladimir Kren, Email: kren@biomed.cas.cz.

Ludmila Martinkova, Email: martinko@biomed.cas.cz.

References

  1. Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L. Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. BMC Biotechnol. 2011;11:2. doi: 10.1186/1472-6750-11-2. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]

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