Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1977 Aug;60(2):429–434. doi: 10.1172/JCI108792

Relationship between bile flow and Na+, K+-adenosinetriphosphatase in liver plasma membranes enriched in bile canaliculi.

J Reichen, G Paumgartner
PMCID: PMC372384  PMID: 141463

Abstract

The relationship between bile flow and Na+,K+-ATPase activity in liver plasma membranes enriched in bile canaliculi was studied in rats treated with ethinyl estradiol, phenobarbital, or 20-methyl cholanthrene. In comparison with controls (1.49+/-0.12 microliter/min per g liver), bile flow was significantly diminished by ethinyl estradiol, increased by phenobarbital, and unchanged by 20-methyl cholanthrene or the solvent, propanediol (0.92+/-0.31, 2.50+/-0.21, 1.62+/-0.18, and 1.64+/-0.30 microliter/min per g liver, respectively). The corresponding values for canalicular Na+,K+-ATPase activity were 80.7+/-19.2, 50.0+/-18.4, 231.7+/-42.6, 82.7+/-30.7, and 143.6+/-55.3 micronmol Pi/h per g liver. Canalicular Na+,K+-ATPase activity was significantly correlated (r=0.785, n=31) with bile flow. These findings support the hypothesis that a fraction of bile flow is related to Na+,K+-ATPase activity and canalicular Na+ transport.

Full text

PDF
429

Images in this article

431Image
on p.431

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avissar N., de Vries A., Ben-Shaul Y., Cohen I. Actin-activated ATPase from human erythrocytes. Biochim Biophys Acta. 1975 Jan 14;375(1):35–43. doi: 10.1016/0005-2736(75)90070-x. [DOI] [PubMed] [Google Scholar]
  2. BODANSKY O., SCHWARTZ M. K. COMPARATIVE EFFECTS OF L-HISTIDINE ON THE ACTIVITIES OF 5'-NUCLEOTIDASE AND ALKALINE PHOSPHATASE. J Biol Chem. 1963 Oct;238:3420–3427. [PubMed] [Google Scholar]
  3. Baudhuin P., Evrard P., Berthet J. Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles. J Cell Biol. 1967 Jan;32(1):181–191. doi: 10.1083/jcb.32.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berthelot P., Erlinger S., Dhumeaux D., Preaux A. M. Mechanism of phenobarbital-induced hypercholeresis in the rat. Am J Physiol. 1970 Sep;219(3):809–813. doi: 10.1152/ajplegacy.1970.219.3.809. [DOI] [PubMed] [Google Scholar]
  5. Boyer J. L. Canalicular bile formation in the isolated perfused rat liver. Am J Physiol. 1971 Oct;221(4):1156–1163. doi: 10.1152/ajplegacy.1971.221.4.1156. [DOI] [PubMed] [Google Scholar]
  6. Boyer J. L., Reno D. Properties of (Na+ plus K+)-activated ATPase in rat liver plasma membranes enriched with bile canaliculi. Biochim Biophys Acta. 1975 Aug 5;401(1):59–72. doi: 10.1016/0005-2736(75)90341-7. [DOI] [PubMed] [Google Scholar]
  7. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dhumeaux D., Erlinger S., Benhamou J. P., Fauvert R. Effects of rose bengal on bile secretion in the rabbit: inhibition of a bile salt-independent fraction. Gut. 1970 Feb;11(2):134–140. doi: 10.1136/gut.11.2.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EARL D. C., KORNER A. THE ISOLATION AND PROPERTIES OF CARDIAC RIBOSOMES AND POLYSOMES. Biochem J. 1965 Mar;94:721–734. doi: 10.1042/bj0940721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ERNSTER L., LINDBERG O. Determination of organic phosphorus compounds by phosphate analysis. Methods Biochem Anal. 1956;3:1–22. doi: 10.1002/9780470110195.ch1. [DOI] [PubMed] [Google Scholar]
  11. Erlinger S., Dhumeaux D., Berthelot P., Dumont M. Effect of inhibitors of sodium transport on bile formation in the rabbit. Am J Physiol. 1970 Aug;219(2):416–422. doi: 10.1152/ajplegacy.1970.219.2.416. [DOI] [PubMed] [Google Scholar]
  12. French S. W., Davies P. L. Ultrastructural localization of actin-like filaments in rat hepatocytes. Gastroenterology. 1975 Apr;68(4 Pt 1):765–774. [PubMed] [Google Scholar]
  13. Glynn I. M., Karlish S. J. The sodium pump. Annu Rev Physiol. 1975;37:13–55. doi: 10.1146/annurev.ph.37.030175.000305. [DOI] [PubMed] [Google Scholar]
  14. Graf J., Korn P., Peterlik M. Choleretic effects of ouabain and ethacrynic acid in the isolated perfused rat liver. Naunyn Schmiedebergs Arch Pharmacol. 1972;272(2):230–233. doi: 10.1007/BF00508771. [DOI] [PubMed] [Google Scholar]
  15. Graf J., Peterlik M. Ouabain-mediated sodium uptake and bile formation by isolated perfused rat liver. Am J Physiol. 1976 Apr;230(4):876–885. doi: 10.1152/ajplegacy.1976.230.4.876. [DOI] [PubMed] [Google Scholar]
  16. Gumucio J. J., Valdivieso V. D. Studies on the mechanism of the ethynylestradiol impairment of bile flow and bile salt excretion in the rat. Gastroenterology. 1971 Sep;61(3):339–344. [PubMed] [Google Scholar]
  17. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  18. Heikel T. A., Lathe G. H. The effect of 17-alpha-ethinyl-substituted steroids on adenosine triphosphatases of rat liver plasma membrane. Biochem J. 1970 Jun;118(1):187–189. doi: 10.1042/bj1180187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Klaassen C. D. Biliary flow after microsomal enzyme induction. J Pharmacol Exp Ther. 1969 Aug;168(2):218–223. [PubMed] [Google Scholar]
  20. Laperche Y., Launay A., Oudéa P. Effects of phenobarbital and rose bengal on the ATPases of plasma membranes of rat and rabbit liver. Gut. 1972 Nov;13(11):920–925. doi: 10.1136/gut.13.11.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Layden T. J., Boyer J. L. The effect of thyroid hormone on bile salt-independent bile flow and Na+, K+ -ATPase activity in liver plasma membranes enriched in bile canaliculi. J Clin Invest. 1976 Apr;57(4):1009–1018. doi: 10.1172/JCI108342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Neville D. M., Jr Isolation of an organ specific protein antigen from cell-surface membrane of rat liver. Biochim Biophys Acta. 1968 Apr 9;154(3):540–552. doi: 10.1016/0005-2795(68)90014-7. [DOI] [PubMed] [Google Scholar]
  23. Ray T. K. A modified method for the isolation of the plasma membrane from rat liver. Biochim Biophys Acta. 1970 Jan 6;196(1):1–9. doi: 10.1016/0005-2736(70)90159-8. [DOI] [PubMed] [Google Scholar]
  24. Simon F. R., Arias I. M. Alteration of bile canalicular enzymes in cholestasis. A possible cause of bile secretory failure. J Clin Invest. 1973 Apr;52(4):765–775. doi: 10.1172/JCI107239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Song C. S., Rubin W., Rifkind A. B., Kappas A. Plasma membranes of the rat liver. Isolation and enzymatic characterization of a fraction rich in bile canaliculi. J Cell Biol. 1969 Apr;41(1):124–132. doi: 10.1083/jcb.41.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WHEELER K. P., WHITTAM R. Fome properties of a kidney adenosine triphosphatase relevant to active cation transport. Biochem J. 1962 Dec;85:495–507. doi: 10.1042/bj0850495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wisher M. H., Evans W. H. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte. Biochem J. 1975 Feb;146(2):375–388. doi: 10.1042/bj1460375. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES