Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1977 Aug;60(2):449–454. doi: 10.1172/JCI108795

A New Antigen System Expressed in Human Endothelial Cells

J Roberto Moraes 1,2, Peter Stastny 1,2
PMCID: PMC372387  PMID: 874103

Abstract

Kidney transplant recipients were previously found to have antibodies that reacted with cells isolated from the endothelium of umbilical cord veins and which were not cytotoxic for lymphocytes from the same donors. Results of the present experiments indicate that endothelial (E) antigens are different from previously known HLA antigens and also from Ia-like antigens of bone marrow-derived (B) lymphocytes. Attempts to absorb E antibodies with lymphocytes from E-positive donors failed in most cases. Antigen redistribution experiments showed that E antigens were located in separate molecules from the products of HLA-A, B, and C. Thus, E cells treated with E antibody became resistant to lysis by the antibody used, but remained susceptible to the effects of typing sera for alleles of HLA-A, B, and C. Antibodies to E cells were also cytotoxic for blood monocytes. Moreover, monocytes were able to absorb E-antibody reactions, indicating that similar antigens were expressed in both cells. E antibodies did not react with B lymphocytes isolated from peripheral blood. In that regard E antibodies were different from antibodies to human Ia-like antigens which reacted with E cells, monocytes, and isolated B lymphocytes. Thus it appears that E antigens constitute a system of human alloantigens which has not been previously identified. The possibility that these antigens play a role in kidney allograft rejection should now be investigated since matching can be performed using monocytes isolated from the blood of recipients and donors.

Full text

PDF
449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen F. H., Jr Linkage of HL-A and GBG. Vox Sang. 1974;27(4):382–384. doi: 10.1111/j.1423-0410.1974.tb02433.x. [DOI] [PubMed] [Google Scholar]
  2. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  3. Boyum A. Separation of blood leucocytes, granulocytes and lymphocytes. Tissue Antigens. 1974;4(4):269–274. [PubMed] [Google Scholar]
  4. Buckley C. E., 3rd, Dorsey F. C., Corley R. B., Ralph W. B., Woodbury M. A., Amos D. B. HL-A-linked human immune-response genes. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2157–2161. doi: 10.1073/pnas.70.7.2157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Busch G. J., Galvanek E. G., Reynolds E. S., Jr Human renal allografts. Analysis of lesions in long-term survivors. Hum Pathol. 1971 Jun;2(2):253–298. doi: 10.1016/s0046-8177(71)80037-0. [DOI] [PubMed] [Google Scholar]
  6. Dorf M. E., Balner H., Benacerraf B. Mapping of the immune response genes in the major histocompatibility complex of the Rhesus monkey. J Exp Med. 1975 Sep 1;142(3):673–693. doi: 10.1084/jem.142.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Einstein L. P., Alper C. A., Bloch K. J., Herrin J. T., Rosen F. S., David J. R., Colten H. R. Biosynthetic defect in monocytes from human beings with genetic deficiency of the second component of complement. N Engl J Med. 1975 May 29;292(22):1169–1171. doi: 10.1056/NEJM197505292922207. [DOI] [PubMed] [Google Scholar]
  8. Festenstein H., Sachs J. A., Pegrum G. D., Moorhead J. F., Paris A. M. Influence of HLA matching and blood-transfusion on outcome of 502 London Transplant Group renal-graft recipients. Lancet. 1976 Jan 24;1(7952):157–161. doi: 10.1016/s0140-6736(76)91270-8. [DOI] [PubMed] [Google Scholar]
  9. Fu S. M., Kunkel H. G., Brusman H. P., Allen F. H., Jr, Fotino M. Evidence for linkage between HL-A histocompatibility genes and those involved in the synthesis of the second component of complement. J Exp Med. 1974 Oct 1;140(4):1108–1111. doi: 10.1084/jem.140.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hauptfeld V., Hauptfeld M., Klein J. Induction of resistance to antibody-mediated cytotoxicity. H-2, Ia, and Ig antigens are independent entities in the membrane of mouse lymphocytes. J Exp Med. 1975 May 1;141(5):1047–1056. doi: 10.1084/jem.141.5.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haverkorn M. J., Hofman B., Masurel N., Rood J. J. HL-A linked genetic control of immune response in man. Transplant Rev. 1975;22:120–124. doi: 10.1111/j.1600-065x.1975.tb01554.x. [DOI] [PubMed] [Google Scholar]
  12. Hirschberg H., Evensen S. A., Henriksen T., Thorsby E. Stimulation of human lymphocytes by allogeneic endothelial cells in vitro. Tissue Antigens. 1974;4(3):257–261. doi: 10.1111/j.1399-0039.1974.tb00249.x. [DOI] [PubMed] [Google Scholar]
  13. Jeannet M., Pinn V. W., Flax M. H., Winn H. J., Russell P. S. Humoral antibodies in renal allotransplantation in man. N Engl J Med. 1970 Jan 15;282(3):111–117. doi: 10.1056/NEJM197001152820301. [DOI] [PubMed] [Google Scholar]
  14. Jones E. A., Goodfellow P. N., Bodmer J. G., Bodmer W. F. Serological identification of HL-A-linked human "Ia-type" antigens. Nature. 1975 Aug 21;256(5519):651–652. doi: 10.1038/256650a0. [DOI] [PubMed] [Google Scholar]
  15. Moraes J. R., Stastny P. Eight groups of human endothelial cell alloantigens. Tissue Antigens. 1976 Oct;8(4):273–276. [PubMed] [Google Scholar]
  16. Perkins H. A., Gantan Z., Siegel S., Howell E., Belzer F. O., Kountz S. L. Reactions of kidney cells with cytotoxic antisera: possible evidence of kidney-specific antigens. Tissue Antigens. 1975 Apr;5(2):89–98. [PubMed] [Google Scholar]
  17. Schirrmacher V., Peña-Martinez J., Festenstein H. Specific lymphocyte-activating determinants expressed on mouse macrophages. Nature. 1975 May 8;255(5504):155–156. doi: 10.1038/255155a0. [DOI] [PubMed] [Google Scholar]
  18. Touraine J. L., Touraine F., Kiszkiss D. F., Choi Y. S., Good R. A. Heterologous specific antiserum for identification of human T lymphocytes. Clin Exp Immunol. 1974 Apr;16(4):503–520. [PMC free article] [PubMed] [Google Scholar]
  19. Vetto R. M., Burger D. R. The identification and comparison of transplantation antigens on canine vascular endothelium and lymphocytes. Transplantation. 1971 Apr;11(4):374–377. doi: 10.1097/00007890-197104000-00003. [DOI] [PubMed] [Google Scholar]
  20. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]
  21. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]
  22. YOUNG M. R., SMITH A. U. THE USE OF EUCHRYSINE IN STAINING CELLS AND TISSUES FOR FLUORESCENCE MICROSCOPY. J R Microsc Soc. 1964 Mar;82:233–244. doi: 10.1111/j.1365-2818.1964.tb04477.x. [DOI] [PubMed] [Google Scholar]
  23. van Rood J. J., van Leeuwen A., Keuning J. J., van Oud Alblas A. B. The serological recognition of the human MLC determinants using a modified cytotoxicity technique. Tissue Antigens. 1975 Apr;5(2):73–79. doi: 10.1111/j.1399-0039.1975.tb00532.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES