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. 1977 Sep;60(3):702–708. doi: 10.1172/JCI108822

Regulation of Mammary and Adipose Tissue Lipoprotein Lipase and Blood Triacylglycerol in Rats during Late Pregnancy

EFFECT OF PROSTAGLANDINS

Peter M Spooner 1, Mary M Garrison 1, Robert O Scow 1
PMCID: PMC372415  PMID: 893673

Abstract

The effects of several prostaglandins on lipoprotein lipase activity of mammary gland and adipose tissue and serum triacylglycerol were studied during late pregnancy in rats. Prostaglandins were injected twice daily for 2 days before and once on the day of analysis. In rats pregnant 20 days, prostaglandin F2α (PGF2α) increased the activity of lipoprotein lipase in mammary gland fourfold, reduced the activity in adipose tissue about 60%, and decreased serum concentration of triacylglycerol 50%. PGF2α also reduced serum concentration of progesterone 90% and increased that of prolactin fivefold, but had no effect on serum concentrations of either immuno-reactive insulin or 17β-estradiol. Injections of 13,14-dihydro-15-keto PGF2α, a metabolite of PGF2α, had similar effects in rats pregnant 20 days, whereas prostaglandins E1 and E2 did not. In rats pregnant 16 days, PGF2α did not affect lipoprotein lipase activity in the tissues or the concentration of triacylglycerol and prolactin in serum, although it decreased serum progesterone 80%.

2-Br-α-ergocryptine prevented the increase in serum prolactin in response to PGF2α, but did not alter the effect of PGF2α on lipoprotein lipase activity or serum triacylglycerol. Progesterone completely blocked the effects of PGF2α on lipoprotein lipase activity and serum triacylglycerol and prolactin concentrations.

These findings indicate that the changes in lipoprotein lipase activity and serum triacylglycerol in PGF2α-treated rats are probably related to the inhibitory action of PGF2α on progesterone secretion. They also suggest that endogenous F prostaglandins may play a role in the regulation of lipoprotein lipase activity in mammary gland and adipose tissue near parturition.

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Selected References

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  1. Abraham G. E., Swerdloff R., Tulchinsky D., Odell W. D. Radioimmunoassay of plasma progesterone. J Clin Endocrinol Metab. 1971 May;32(5):619–624. doi: 10.1210/jcem-32-5-619. [DOI] [PubMed] [Google Scholar]
  2. Amenomori Y., Chen C. L., Meites J. Serum prolactin levels in rats during different reproductive states. Endocrinology. 1970 Mar;86(3):506–510. doi: 10.1210/endo-86-3-506. [DOI] [PubMed] [Google Scholar]
  3. BORGSTROM B. Investigation on lipid separation methods. Separation of cholesterol esters, glycerides and free fatty acids. Acta Physiol Scand. 1952 Jun 6;25(2-3):111–119. doi: 10.1111/j.1748-1716.1952.tb00863.x. [DOI] [PubMed] [Google Scholar]
  4. Borensztajn J., Samols D. R., Rubenstein A. H. Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue. Am J Physiol. 1972 Dec;223(6):1271–1275. doi: 10.1152/ajplegacy.1972.223.6.1271. [DOI] [PubMed] [Google Scholar]
  5. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis J. W., Wikman-Coffelt J., Eddington C. L. The effect of progesterone on biosynthetic pathways in mammary tissue. Endocrinology. 1972 Oct;91(4):1011–1019. doi: 10.1210/endo-91-4-1011. [DOI] [PubMed] [Google Scholar]
  7. Döhler K. D., Wuttke W. Total blockade of phasic pituitary prolactin release in rats: effect on serum LH and progesterone during the estrous cycle and pregnancy. Endocrinology. 1974 Jun;94(6):1595–1600. doi: 10.1210/endo-94-6-1595. [DOI] [PubMed] [Google Scholar]
  8. Enser M. Clearing-factor lipase in obese hyperglycaemic mice (ob-ob). Biochem J. 1972 Sep;129(2):447–453. doi: 10.1042/bj1290447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gautvik K. M., Kriz M. Effects of prostaglandins on prolactin and growth hormone synthesis and secretion in cultured rat pituitary cells. Endocrinology. 1976 Feb;98(2):352–358. doi: 10.1210/endo-98-2-352. [DOI] [PubMed] [Google Scholar]
  10. Goldberg V. J., Ramwell P. W. Role of rostaglandins in reproduction. Physiol Rev. 1975 Jul;55(3):325–351. doi: 10.1152/physrev.1975.55.3.325. [DOI] [PubMed] [Google Scholar]
  11. Hamosh M., Clary T. R., Chernick S. S., Scow R. O. Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970 Sep 8;210(3):473–482. doi: 10.1016/0005-2760(70)90044-5. [DOI] [PubMed] [Google Scholar]
  12. Hamosh M., Hamosh P. The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue. J Clin Invest. 1975 May;55(5):1132–1135. doi: 10.1172/JCI108015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelly P. A., Shiu R. P., Robertson M. C., Friesen H. G. Characterization of rat chorionic mammotropin. Endocrinology. 1975 May;96(5):1187–1195. doi: 10.1210/endo-96-5-1187. [DOI] [PubMed] [Google Scholar]
  14. Kieffer J. D., Weintraub B. D., Baigelman W., Leeman S., Maloof F. Homologous radioimmunoassay of thyrotrophin in rat plasma. Acta Endocrinol (Copenh) 1974 Jul;76(3):495–505. doi: 10.1530/acta.0.0760495. [DOI] [PubMed] [Google Scholar]
  15. Kim H. J., Kalkhoff R. K. Sex steroid influence on triglyceride metabolism. J Clin Invest. 1975 Oct;56(4):888–896. doi: 10.1172/JCI108168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knopp R. H., Saudek C. D., Arky R. A., O'Sullivan J. B. 2 phases of adipose tissue metabolism in pregnancy: maternal adaptations for fetal growth. Endocrinology. 1973 Apr;92(4):984–988. doi: 10.1210/endo-92-4-984. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Labhsetwar A. P., Watson D. J. Temporal relationship between secretory patterns of gonadotropins, estrogens, and prostaglandin-F in periparturient rats. Biol Reprod. 1974 Feb;10(1):103–110. doi: 10.1095/biolreprod10.1.103. [DOI] [PubMed] [Google Scholar]
  19. Loriaux D. L., Ruder H. J., Lipsett M. B. The measurement of estrone sulfate in plasma. Steroids. 1971 Oct;18(4):463–472. doi: 10.1016/0039-128x(71)90059-6. [DOI] [PubMed] [Google Scholar]
  20. Morishige W. K., Pepe G. J., Rothchild I. Serum luteinizing hormone, prolactin and progesterone levels during pregnancy in the rat. Endocrinology. 1973 May;92(5):1527–1530. doi: 10.1210/endo-92-5-1527. [DOI] [PubMed] [Google Scholar]
  21. Murphy G., Ariyanayagam A. D., Kuhn N. J. Progesterone and the metabolic control of the lactose biosynthetic pathway during lactogenesis in the rat. Biochem J. 1973 Dec;136(4):1105–1116. doi: 10.1042/bj1361105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Otway S., Robinson D. S. The significance of changes in tissue clearing-factor lipase activity in relation to the lipaemia of pregnancy. Biochem J. 1968 Feb;106(3):677–682. doi: 10.1042/bj1060677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pharriss B. Prostaglandin induction of luteolysis. Ann N Y Acad Sci. 1971 Apr 30;180:436–444. doi: 10.1111/j.1749-6632.1971.tb53211.x. [DOI] [PubMed] [Google Scholar]
  24. Pykälistö O. J., Smith P. H., Brunzell J. D. Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity. J Clin Invest. 1975 Nov;56(5):1108–1117. doi: 10.1172/JCI108185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. RAPPORT M. M., ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed] [Google Scholar]
  26. Rodbard D., Frazier G. R. Statistical analysis of radioligand assay data. Methods Enzymol. 1975;37:3–22. doi: 10.1016/s0076-6879(75)37003-1. [DOI] [PubMed] [Google Scholar]
  27. Samuelsson B., Granström E., Green K., Hamberg M., Hammarström S. Prostaglandins. Annu Rev Biochem. 1975;44:669–695. doi: 10.1146/annurev.bi.44.070175.003321. [DOI] [PubMed] [Google Scholar]
  28. Scow R. O., Blanchette-Mackie E. J., Smith L. C. Role of capillary endothelium in the clearance of chylomicrons. A model for lipid transport from blood by lateral diffusion in cell membranes. Circ Res. 1976 Aug;39(2):149–162. doi: 10.1161/01.res.39.2.149. [DOI] [PubMed] [Google Scholar]
  29. Shemesh M., Hansel W. Levels of prostaglandin F (PGF) in bovine endometrium uterine venous, ovarian arterial and jugular plasma during the estrous cycle (38789). Proc Soc Exp Biol Med. 1975 Jan;148(1):123–126. doi: 10.3181/00379727-148-38489. [DOI] [PubMed] [Google Scholar]
  30. Smith I. D., Shearman R. P., Korda A. R. Lactation following therapeutic abortion with prostaglandin F 2 . Nature. 1972 Dec 15;240(5381):411–412. doi: 10.1038/240411a0. [DOI] [PubMed] [Google Scholar]
  31. Strauss J. F., 3rd, Stambaugh R. L. Induction of 20 alpha-hydroxysteroid dehydrogenase in rat corpora lutea of pregnancy by prostaglandin F-2 alpha. Prostaglandins. 1974 Jan 10;5(1):73–85. doi: 10.1016/s0090-6980(74)80134-6. [DOI] [PubMed] [Google Scholar]
  32. Vermouth N. T., Deis R. P. Inhibitory effect of progesterone on the lactogenic and abortive action of prostaglandin F2alpha. J Endocrinol. 1975 Jul;66(1):21–29. doi: 10.1677/joe.0.0660021. [DOI] [PubMed] [Google Scholar]
  33. Vermouth N. T., Deis R. P. Prolactin release induced by prostaglandin F2 in pregnant rats. Nat New Biol. 1972 Aug 23;238(86):248–250. doi: 10.1038/newbio238248a0. [DOI] [PubMed] [Google Scholar]
  34. Zinder O., Hamosh M., Fleck T. R., Scow R. O. Effect of prolactin on lipoprotein lipase in mammary glands and adipose tissue of rats. Am J Physiol. 1974 Mar;226(3):742–748. doi: 10.1152/ajplegacy.1974.226.3.744. [DOI] [PubMed] [Google Scholar]

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