Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1977 Dec;60(6):1348–1353. doi: 10.1172/JCI108894

Inhibition of vasopressin-stimulated prostaglandin E biosynthesis by chlorpropamide in the toad urinary bladder. Mechanism of enhancement of vasopressin-stimulated water flow.

R M Zusman, H R Keiser, J S Handler
PMCID: PMC372491  PMID: 199621

Abstract

Chlorpropamide is known to enhance the water permeability response of the toad urinary bladder to vasopressin and to theophylline. In other studies, we have shown that prostaglandin E synthesis by the toad bladder inhibits the water permeability response to arginine vasopressin and to theophylline. In this study, the effect of chlorpropamide on vasopressin-, theophylline-, and cyclic AMP-stimulated water flow and on prostaglandin E biosynthesis was investigated in the toad urinary bladder in vitro. Chlorpropamide inhibited prostaglandin E biosynthesis during vasopressin-, theophylline- and cyclic AMP-stimulated water flow. Tolbutamide and glyburide, two other sulfonylurea compounds, also enhanced vasopressin-stimulated water flow and inhibited vasopressin-stimulated prostaglandin E biosynthesis. We conclude that the mechanism of enhancement on vasopressin-stimulated water flow by the sulfonylureas is the inhibition of prostaglandin E biosynthesis.

Full text

PDF
1348

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arduino F., Ferraz F. P., Rodrigues J. Antidiuretic action of chlorpropamide in idiopathic diabetes insipidus. J Clin Endocrinol Metab. 1966 Dec;26(12):1325–1328. doi: 10.1210/jcem-26-12-1325. [DOI] [PubMed] [Google Scholar]
  2. Ingelfinger J. R., Hays R. M. Evidence that chlorpropamide and vasopressin share a common site of action. J Clin Endocrinol Metab. 1969 May;29(5):738–740. doi: 10.1210/jcem-29-5-738. [DOI] [PubMed] [Google Scholar]
  3. Lipson L., Hynie S., Sharp G. Effect of prostaglandin E-1 on osmotic water flow and sodium transport in the toad bladder. Ann N Y Acad Sci. 1971 Apr 30;180:260–277. doi: 10.1111/j.1749-6632.1971.tb53196.x. [DOI] [PubMed] [Google Scholar]
  4. Lozada E. S., Gouaux J., Franki N., Appel G. B., Hays R. M. Studies of the mode of action of the sulfonylureas and phenylacetamides in enhancing the effect of vasopressin. J Clin Endocrinol Metab. 1972 Apr;34(4):704–712. doi: 10.1210/jcem-34-4-704. [DOI] [PubMed] [Google Scholar]
  5. Mendoza S. A., Brown C. F., Jr Effect of chlorpropamide on osmotic water flow across toad bladder and the response to vasopressin, theophylline and cyclic AMP. J Clin Endocrinol Metab. 1974 May;38(5):883–889. doi: 10.1210/jcem-38-5-883. [DOI] [PubMed] [Google Scholar]
  6. Mendoza S. A. Effect of chlorpropamide on the permeability of the urinary bladder of the toad and the response to vasopressin, adenosine-3',5'-monophosphate and theophylline. Endocrinology. 1969 Feb;84(2):411–414. doi: 10.1210/endo-84-2-411. [DOI] [PubMed] [Google Scholar]
  7. Mendoza S. A., Handler J. S., Orloff J. Effect of inhibitors of sodium transport on response of toad bladder to ADH and cyclic AMP. Am J Physiol. 1970 Nov;219(5):1440–1445. doi: 10.1152/ajplegacy.1970.219.5.1440. [DOI] [PubMed] [Google Scholar]
  8. Moses A. M., Numann P., Miller M. Mechanism of chlorpropamide-induced antidiuresis in man: evidence for release of ADH and enhancement of peripheral action. Metabolism. 1973 Jan;22(1):59–66. doi: 10.1016/0026-0495(73)90029-2. [DOI] [PubMed] [Google Scholar]
  9. ORLOFF J., HANDLER J. S., BERGSTROM S. EFFECT OF PROSTAGLANDIN (PGE-1) ON THE PERMEABILITY RESPONSE OF TOAD BLADDER TO VASOPRESSIN, THEOPHYLLINE AND ADENOSINE 3',5'-MONOPHOSPHATE. Nature. 1965 Jan 23;205:397–398. doi: 10.1038/205397a0. [DOI] [PubMed] [Google Scholar]
  10. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Omachi R. S., Robbie D. E., Handler J. S., Orloff J. Effects of ADH and other agents on cyclic AMP accumulation in toad bladder epithelium. Am J Physiol. 1974 May;226(5):1152–1157. doi: 10.1152/ajplegacy.1974.226.5.1152. [DOI] [PubMed] [Google Scholar]
  12. Urakabe S., Shirai D. Effect of vasopressin, cyclic 3',5'-AMP, and chlorpropamide on water permeability of toad urinary bladder. Med J Osaka Univ. 1971 Mar;21(2):151–159. [PubMed] [Google Scholar]
  13. Vargaftig B. B., Hai N. D. Selective inhibition by mepacrine of the release of "rabbit aorta contracting substance" evoked by the administration of bradykinin. J Pharm Pharmacol. 1972 Feb;24(2):159–161. doi: 10.1111/j.2042-7158.1972.tb08953.x. [DOI] [PubMed] [Google Scholar]
  14. Webster B., Bain J. Antidiuretic effect and complications of chlorpropamide therapy in diabetes insipidus. J Clin Endocrinol Metab. 1970 Feb;30(2):215–227. doi: 10.1210/jcem-30-2-215. [DOI] [PubMed] [Google Scholar]
  15. YALOW R. S., BLACK H., VILLAZON M., BERSON S. A. Comparison of plasma insulin levels following administration of tolbutamide and glucose. Diabetes. 1960 Sep-Oct;9:356–362. doi: 10.2337/diab.9.5.356. [DOI] [PubMed] [Google Scholar]
  16. Zusman R. M., Keiser H. R., Handler J. S. Vasopressin-stimulated prostaglandin E biosynthesis in the toad urinary bladder. Effect of water flow. J Clin Invest. 1977 Dec;60(6):1339–1347. doi: 10.1172/JCI108893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zusman R. M., Keiser H. R. Prostaglandin E2 biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Mechanism of stimulation by angiotensin II, bradykinin, and arginine vasopressin. J Biol Chem. 1977 Mar 25;252(6):2069–2071. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES