Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1978 Feb;61(2):370–380. doi: 10.1172/JCI108947

The Actions of Secretagogues on Oxygen Uptake by Isolated Mammalian Parietal Cells

Andrew H Soll 1,2
PMCID: PMC372547  PMID: 621277

Abstract

The action of histamine, carbamylcholine, and gastrin on oxygen uptake by cells isolated from canine fundic mucosa was studied in vitro. Viable mucosal cells were prepared by exposure of separated mucosa sequentially to collagenase and EDTA. Oxygen consumption, determined by polarography, was chosen as an index of physiological response of mucosal cells to secretagogues.

Isobutyl methyl xanthine (IMX), carbamylcholine, histamine, and gastrín each independently stimulated oxygen uptake by the unfractionated mucosal cells. The response to histamine was greatly enhanced when IMX was present. In fractions of varying parietal cell content obtained with the Beckman elutriator rotor, basal and stimulated oxygen uptake correlated with the parietal cell content of the fractions. The percentage increases in oxygen uptake in response to histamine, gastrin, carbamylcholine, and IMX were similar in enriched fractions with from 50 to 85% parietal cells and in unenriched starting fractions. The normalized dose-response relations for histamine with an IMX background and for carbamylcholine were also similar in these two fractions.

The specificity of these responses was tested by use of an H2-histamine receptor antagonist, metiamide, and an anticholinergic agent, atropine. At the doses used, neither metiamide (0.1 mM) nor atropine (10 μM) inhibited basal oxygen uptake. Histamine, studied with an IMX background, was inhibited by metiamide but not by atropine, while carbamylcholine was inhibited by atropine but not by metiamide. Neither metiamide nor atropine inhibited gastrin-stimulated oxygen uptake.

These data indicate that in this in vitro system parietal cells account for most of the increase in oxygen uptake produced by exposure to gastric secretagogues and that histamine, gastrin, and carbamylcholine each independently stimulate oxygen uptake by the parietal cell. The specificity displayed by atropine and metiamide in this in vitro system suggests that the parietal cell has specific receptors for each of these secretagogues.

Full text

PDF
370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALONSO D., HARRIS J. B. EFFECT OF XANTHINES AND HISTAMINE ON ION TRANSPORT AND RESPIRATION BY FROG GASTRIC MUCOSA. Am J Physiol. 1965 Jan;208:18–23. doi: 10.1152/ajplegacy.1965.208.1.18. [DOI] [PubMed] [Google Scholar]
  2. Amsterdam A., Jamieson J. D. Studies on dispersed pancreatic exocrine cells. I. Dissociation technique and morphologic characteristics of separated cells. J Cell Biol. 1974 Dec;63(3):1037–1056. doi: 10.1083/jcb.63.3.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berglindh T., Helander H. F., Obrink K. J. Effects of secretagogues on oxygen consumption, aminopyrine accumulation and morphology in isolated gastric glands. Acta Physiol Scand. 1976 Aug;97(4):401–414. doi: 10.1111/j.1748-1716.1976.tb10281.x. [DOI] [PubMed] [Google Scholar]
  4. Blum A. L., Shah G. T., Wiebelhaus V. D., Brennan F. T., Helander H. F., Ceballos R., Sachs G. Pronase method for isolation of viable cells from Necturus gastric mucosa. Gastroenterology. 1971 Aug;61(2):189–200. [PubMed] [Google Scholar]
  5. Chapman R. A., Miller D. J. Structure-activity relations for caffeine: a comparative study of the inotropic effects of the methylxanthines, imidazoles and related compounds on the frog's heart. J Physiol. 1974 Nov;242(3):615–634. doi: 10.1113/jphysiol.1974.sp010726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Code C. F. Histamine and gastric secretion: a later look, 1955-1965. Fed Proc. 1965 Nov-Dec;24(6):1311–1321. [PubMed] [Google Scholar]
  7. Croft D. N., Ingelfinger F. J. Isolated gastric parietal cells: oxygen consumption, electrolyte content and intracellular pH. Clin Sci. 1969 Oct;37(2):491–501. [PubMed] [Google Scholar]
  8. Glick D. M. Stimulated chloride transport by isolated parietal cells. Biochem Pharmacol. 1974 Dec 1;23(23):3283–3288. doi: 10.1016/0006-2952(74)90651-0. [DOI] [PubMed] [Google Scholar]
  9. Hersey S. J. Interactions between oxidative metabolism and acid secretion in gastric mucosa. Biochim Biophys Acta. 1974 Sep 16;344(2):157–203. doi: 10.1016/0304-4157(74)90003-3. [DOI] [PubMed] [Google Scholar]
  10. Kasbekar D. K. Studies of resting isolated frog gastric mucosa. Proc Soc Exp Biol Med. 1967 May;125(1):267–271. doi: 10.3181/00379727-125-32066. [DOI] [PubMed] [Google Scholar]
  11. Kowalewski K., Kolodej A. Relation between hydrogen ion secretion and oxygen consumption by ex vivo isolated canine stomach, perfused with homologous blood. Can J Physiol Pharmacol. 1972 Oct;50(10):955–961. doi: 10.1139/y72-138. [DOI] [PubMed] [Google Scholar]
  12. Lewin M., Cheret A. M., Soumarmon A., Girodet J. Méthode pour l'isolement et le tri des cellules de la muqueuse fundique de rat. Biol Gastroenterol (Paris) 1974 Apr-May;7(2):139–144. [PubMed] [Google Scholar]
  13. McDogual W. S., DeCosse J. J. Method for determining differential secretory function of isolated cells in vitro: chloride movement in isolated parietal cells. Exp Cell Res. 1970 Jul;61(1):203–206. doi: 10.1016/0014-4827(70)90275-2. [DOI] [PubMed] [Google Scholar]
  14. McEwen C. R., Stallard R. W., Juhos E. T. Separation of biological particles by centrifugal elutriation. Anal Biochem. 1968 Jun;23(3):369–377. doi: 10.1016/0003-2697(68)90228-5. [DOI] [PubMed] [Google Scholar]
  15. McQuarrie D. G., Eichenholz A., Blumentals A. S., Vennes J. A. Kinetics of gastric juice secretion: a correlation of arteriovenous differences with the composition of the gastric juice. Surgery. 1967 Sep;62(3):475–486. [PubMed] [Google Scholar]
  16. Romrell L. J., Coppe M. R., Munro D. R., Ito S. Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation. J Cell Biol. 1975 May;65(2):428–438. doi: 10.1083/jcb.65.2.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sedar A. W. Fine structure of the stimulated oxyntic cell. Fed Proc. 1965 Nov-Dec;24(6):1360–1367. [PubMed] [Google Scholar]
  18. Shoemaker R. L., Hirschowitz B. I., Sachs G. Hormonal stimulation of Necturus gastric mucosa in vitro. Am J Physiol. 1967 May;212(5):1013–1016. doi: 10.1152/ajplegacy.1967.212.5.1013. [DOI] [PubMed] [Google Scholar]
  19. Soll A. H. The interaction of histamine with gastrin and carbamylcholine on oxygen uptake by isolated mammalian parietal cells. J Clin Invest. 1978 Feb;61(2):381–389. doi: 10.1172/JCI108948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WALDER A. I., LUNSETH J. B. A technic for separation of the cells of the gastric mucosa. Proc Soc Exp Biol Med. 1963 Feb;112:494–496. doi: 10.3181/00379727-112-28086. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES