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. 1978 Mar;61(3):781–789. doi: 10.1172/JCI108992

Vagal, Cholinergic Regulation of Pancreatic Polypeptide Secretion

T W Schwartz 1,2,3, J J Holst 1,2,3, J Fahrenkrug 1,2,3, S Lindkær Jensen 1,2,3, O V Nielsen 1,2,3, J F Rehfeld 1,2,3, O B Schaffalitzky de Muckadell 1,2,3, F Stadil 1,2,3
PMCID: PMC372593  PMID: 641155

Abstract

The effect of efferent, parasympathetic stimulation upon pancreatic polypeptide (PP) secretion was studied in three ways: (a) Plasma PP concentrations increased in response to insulin-induced hypoglycemia in both normal subjects, from 11 pM (9.5-12.5) to 136 pM (118-147), n = 8 (median and interquartile range) and in duodenal ulcer patients, from 33 pM (21-52) to 213 pM (157-233), n = 7. The PP response to hypoglycemia was diminished by atropine in normal subjects (P < 0.005) and completely abolished by vagotomy in the duodenal ulcer patients. (b) Electrical stimulation, 8 Hz, of the vagal nerves in anesthetized pigs induced an increase in portal PP concentrations within 30 s from 32 pM (28-39) to 285 pM (248-294), n = 12. Minimal stimulatory frequency was 0.5 Hz and maximal stimulatory frequency 8-12 Hz. Atropine inhibited the PP response to electrical stimulation. Median inhibition with 0.5 mg of atropine/kg body wt was 74%, range 31-90%, n = 6. The response was eliminated by hexamethonium. Adrenergic alpha and beta blockade did not influence the release of PP in response to vagal stimulation. (c) Acetylcholine stimulated, in a dose-dependent manner, the secretion of PP from the isolated perfused porcine pancreas, half-maximal effective dose being 0.19 μM; maximal PP output in response to 5 min stimulation was 228 pmol, range 140-342 pmol, n = 5. Atropine completely abolished this response.

The results of the present study together with the previously demonstrated poor PP response to food in vagotomized patients, indicate that vagal, cholinergic stimulation is a major regulator of PP secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian T. E., Besterman H. S., Cooke T. J., Bloom S. R., Barnes A. J., Russell R. C. Mechanism of pancreatic polypeptide release in man. Lancet. 1977 Jan 22;1(8004):161–163. doi: 10.1016/s0140-6736(77)91762-7. [DOI] [PubMed] [Google Scholar]
  2. Adrian T. E., Bloom S. R., Bryant M. G., Polak J. M., Heitz P. H., Barnes A. J. Distribution and release of human pancreatic polypeptide. Gut. 1976 Dec;17(12):940–944. doi: 10.1136/gut.17.12.940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark A. J. The reaction between acetyl choline and muscle cells: Part II. J Physiol. 1927 Nov 21;64(2):123–143. doi: 10.1113/jphysiol.1927.sp002424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Floyd J. C., Jr, Fajans S. S., Pek S., Chance R. E. A newly recognized pancreatic polypeptide; plasma levels in health and disease. Recent Prog Horm Res. 1976;33:519–570. doi: 10.1016/b978-0-12-571133-3.50019-2. [DOI] [PubMed] [Google Scholar]
  5. Floyd J. C., Jr, Fajans S. S., Pek S. Regulation in healthy subjects of the secretion of human pancreatic polypeptide, a newly recognized pancreatic islet polypeptide. Trans Assoc Am Physicians. 1976;89:146–158. [PubMed] [Google Scholar]
  6. Jensen S. L., Kühl C., Nielsen O. V., Holst J. J. Isolation and perfusion of the porcine pancreas. Scand J Gastroenterol Suppl. 1976;37:57–61. [PubMed] [Google Scholar]
  7. Kimmel J. R., Hayden L. J., Pollock H. G. Isolation and characterization of a new pancreatic polypeptide hormone. J Biol Chem. 1975 Dec 25;250(24):9369–9376. [PubMed] [Google Scholar]
  8. Larsson L. I., Sundler F., Håkanson R. Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells. Cell Tissue Res. 1975;156(2):167–171. doi: 10.1007/BF00221800. [DOI] [PubMed] [Google Scholar]
  9. Larsson L. I., Sundler F., Håkanson R. Pancreatic polypeptide - a postulated new hormone: identification of its cellular storage site by light and electron microscopic immunocytochemistry. Diabetologia. 1976 Jul;12(3):211–226. doi: 10.1007/BF00422088. [DOI] [PubMed] [Google Scholar]
  10. Liedberg G., Nielsen K. C., Owman C., Sjöberg N. O. Adrenergic contribution to the abdominal vagus nerves in the cat. Scand J Gastroenterol. 1973;8(2):177–180. [PubMed] [Google Scholar]
  11. Lin T. M., Evans D. C., Chance R. E., Spray G. F. Bovine pancreatic peptide: action on gastric and pancreatic secretion in dogs. Am J Physiol. 1977 Mar;232(3):E311–E315. doi: 10.1152/ajpendo.1977.232.3.E311. [DOI] [PubMed] [Google Scholar]
  12. Loubatières-Mariani M. M., Chapal J., Alric R., Loubatières A. Studies of the cholinergic receptors involved in the secretion of insulin using isolated perfused rat pancreas. Diabetologia. 1973 Dec;9(6):439–446. doi: 10.1007/BF00461685. [DOI] [PubMed] [Google Scholar]
  13. Said S. I., Rosenberg R. N. Vasoactive intestinal polypeptide: abundant immunoreactivity in neural cell lines and normal nervous tissue. Science. 1976 May 28;192(4242):907–908. doi: 10.1126/science.1273576. [DOI] [PubMed] [Google Scholar]
  14. Schaffalitzky de Muckadell O. B., Fahrenkrug J., Holst J. J. Release of vasoactive intestinal polypeptide (VIP) by electric stimulation of the vagal nerves. Gastroenterology. 1977 Feb;72(2):373–375. [PubMed] [Google Scholar]
  15. Schwartz T. W., Rehfeld J. F., Stadil F., Larson L. I., Chance R. E., Moon N. Pancreatic-polypeptide response to food in duodenal-ulcer patients before and after vagotomy. Lancet. 1976 May 22;1(7969):1102–1105. doi: 10.1016/s0140-6736(76)90065-9. [DOI] [PubMed] [Google Scholar]
  16. Schwartz T. W., Rehfeld J. J. Mechanism of pancreatic-polypeptide release. Lancet. 1977 Mar 26;1(8013):697–698. doi: 10.1016/s0140-6736(77)92132-8. [DOI] [PubMed] [Google Scholar]
  17. Sjödin L. Gastric acid responses to graded vagal stimulation in the anaesthetized cat. Digestion. 1975;12(1):17–24. doi: 10.1159/000197649. [DOI] [PubMed] [Google Scholar]
  18. Stadil F., Malmstrøom J., Rehfeld J. F., Miyata M. Effect of atropine on hypoglycemic release of gastrin in man. Acta Physiol Scand. 1974 Nov;92(3):391–398. doi: 10.1111/j.1748-1716.1974.tb05756.x. [DOI] [PubMed] [Google Scholar]
  19. Stadil F., Rehfeld J. F. Gastrin response to insulin after selective, highly selective, and truncal vagotomy. Gastroenterology. 1974 Jan;66(1):7–15. [PubMed] [Google Scholar]
  20. Wilton A., Joshi P. I. Adrenergic receptors and renin release. Lancet. 1977 Mar 26;1(8013):698–699. doi: 10.1016/s0140-6736(77)92133-x. [DOI] [PubMed] [Google Scholar]

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