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. 1978 Apr;61(4):1044–1047. doi: 10.1172/JCI109003

Polycythemia vera. The in vitro response of normal and abnormal stem cell lines to erythropoietin.

J F Prchal, J W Adamson, S Murphy, L Steinmann, P J Fialkow
PMCID: PMC372621  PMID: 659576

Abstract

Bone marrow cells from two glucose-6-phosphate dehydrogenase (G-6-PD) heterozygotes with polycythemia vera were cultured to determine whether progenitors which wre not of the polycythemia vera clone were present, and, if present, which cell lines contributed to the increase in erythroid colonies observed in response to added erythropoietin (ESF). To accomplish this, the G-6-PD isoenzyme activity of individual erythroid colonies was determined. All of the erythroid colonies analyzed in cultures without added ESF, contained the G-6-PD isoenzyme type characteristic of the abnormal clone. With higher ESF concentrations in the culture, however, there was an increase in the colonies that were not of the polycythemia vera clone. Analysis of the ratio of the various types of colonies indicated that normal and polycythemia vera cells are capable of responding to ESF in vitro. In selected patients, this technique permits analysis of the ratios of normal to abnormal cells during the course of the disease, in response to therapy and during late complications, such as myelofibrosis or leukemic transformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson J. W., Fialkow P. J., Murphy S., Prchal J. F., Steinmann L. Polycythemia vera: stem-cell and probable clonal origin of the disease. N Engl J Med. 1976 Oct 21;295(17):913–916. doi: 10.1056/NEJM197610212951702. [DOI] [PubMed] [Google Scholar]
  2. Adamson J. W., Finch C. A. Erythropoietin and the polycythemias. Ann N Y Acad Sci. 1968 Mar 29;149(1):560–563. doi: 10.1111/j.1749-6632.1968.tb15195.x. [DOI] [PubMed] [Google Scholar]
  3. Adamson J. W. The erythropoietin-hematocrit relationship in normal and polycythemic man: implications of marrow regulation. Blood. 1968 Oct;32(4):597–609. [PubMed] [Google Scholar]
  4. Bomchil G., Carmena A. O., Segade A., Cavagnaro F., De Testa N. G. Studies on the response of hypoxia and relative hyperoxia in two polycythemia vera patients. Indian J Med Res. 1967 Jun;55(6):543–548. [PubMed] [Google Scholar]
  5. Cormack D. Time-lapse characterization of erythrocytic colony-forming cells in plasma cultures. Exp Hematol. 1976 Sep;4(5):319–327. [PubMed] [Google Scholar]
  6. Fialkow P. J., Gartler S. M., Yoshida A. Clonal origin of chronic myelocytic leukemia in man. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1468–1471. doi: 10.1073/pnas.58.4.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fialkow P. J., Jacobson R. J., Papayannopoulou T. Chronic myelocytic leukemia: clonal origin in a stem cell common to the granulocyte, erythrocyte, platelet and monocyte/macrophage. Am J Med. 1977 Jul;63(1):125–130. doi: 10.1016/0002-9343(77)90124-3. [DOI] [PubMed] [Google Scholar]
  8. Fialkow P. J. Primordial cell pool size and lineage relationships of five human cell types. Ann Hum Genet. 1973 Jul;37(1):39–48. doi: 10.1111/j.1469-1809.1973.tb01813.x. [DOI] [PubMed] [Google Scholar]
  9. Golde D. W., Bersch N., Cline M. J. Polycythemia vera: hormonal modulation of erythropoiesis in vitro. Blood. 1977 Mar;49(3):399–405. [PubMed] [Google Scholar]
  10. Golde D. W., Cline M. J. Erythropoietin responsiveness in polycythaemia vera. Br J Haematol. 1975 Apr;29(4):567–573. doi: 10.1111/j.1365-2141.1975.tb02743.x. [DOI] [PubMed] [Google Scholar]
  11. Krantz S. B. Response of polycythemia vera marrow to erythropoietin in vitro. J Lab Clin Med. 1968 Jun;71(6):999–1012. [PubMed] [Google Scholar]
  12. Kurnick J. E., Ward H. P., Block M. H. Bone marrow sections in the differential diagnosis of polycythemia. Arch Pathol. 1972 Dec;94(6):489–499. [PubMed] [Google Scholar]
  13. Prchal J. F., Adamson J. W., Steinmann L., Fialkow P. J. Human erythroid colony formation in vitro: evidence for clonal origin. J Cell Physiol. 1976 Nov;89(3):489–492. doi: 10.1002/jcp.1040890314. [DOI] [PubMed] [Google Scholar]
  14. Prchal J. F., Axelrad A. A. Letter: Bone-marrow responses in polycythemia vera. N Engl J Med. 1974 Jun 13;290(24):1382–1382. doi: 10.1056/nejm197406132902419. [DOI] [PubMed] [Google Scholar]
  15. Sparkes R. S., Baluda M. C., Townsend D. E. Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase. J Lab Clin Med. 1969 Mar;73(3):531–534. [PubMed] [Google Scholar]
  16. Zanjani E. D., Lutton J. D., Hoffman R., Wasserman L. R. Erythroid colony formation by polycythemia vera bone marrow in vitro. Dependence on erythropoietin. J Clin Invest. 1977 May;59(5):841–848. doi: 10.1172/JCI108706. [DOI] [PMC free article] [PubMed] [Google Scholar]

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