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. 1978 May;61(5):1291–1297. doi: 10.1172/JCI109046

Pulmonary Vascular Effects of Fat Emulsion Infusion in Unanesthetized Sheep

PREVENTION BY INDOMETHACIN

Charles R McKeen 1, Kenneth L Brigham 1, Ronald E Bowers 1, Thomas R Harris 1
PMCID: PMC372651  PMID: 659593

Abstract

Pulmonary diffusing capacity and arterial blood Po2 decrease in humans when 10% fat emulsion is infused. To study its effects on the pulmonary circulation and lung fluid balance, we infused 0.25 g/kg × h of a 10% fat emulsion (Intralipid, Cutter Laboratories, Inc., Berkeley, Calif.) into an awake sheep lung lymph preparation. The emulsion caused a sustained increase in pulmonary artery pressure to approximately twice base line with little change in left atrial pressure. PaO2 decreased an average 13 torr and lung lymph flow increased two- to threefold. Lymph/plasma total protein concentration fell as lymph flow increased; the magnitude of the lymph/plasma protein decrease was similar to that reported previously when lung vascular pressures were mechanically elevated. Heparin infusion (loading dose = 4,000 U, maintenance dose = 2,000 U/h) cleared the serum of triglycerides but did not alter the response to fat emulsion. Indomethacin infusion (loading dose = 5 mg/kg, maintenance dose = 3 mg/kg × h) blocked the rise in pulmonary artery pressure, the increase in lung lymph flow, and the fall in PaO2. Neither extravascular lung water nor [14C]urea lung vascular permeability surface area products were altered by fat emulsion infusion. We conclude that fat emulsion infusion in sheep increases lung microvascular filtration by increasing vascular pressures, but has no effect on vascular permeability. Since the effects are blocked by indomethacin, they may be prostaglandin mediated.

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Selected References

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  1. Anderson F. L., Tsagaris T. J., Jubiz W., Kuida H. Prostaglandin F and E levels during endotoxin-induced pulmonary hypertension in calves. Am J Physiol. 1975 May;228(5):1479–1482. doi: 10.1152/ajplegacy.1975.228.5.1479. [DOI] [PubMed] [Google Scholar]
  2. Berry E. M., Edmonds J. F., Wyllie H. Release of prostaglandin E2 and unidentified factors from ventilated lungs. Br J Surg. 1971 Mar;58(3):189–192. doi: 10.1002/bjs.1800580310. [DOI] [PubMed] [Google Scholar]
  3. Borresen H. C., Coran A. G., Knutrud O. Metabolic results of parenteral feeding in neonatal surgery: a balanced parenteral feeding program based on a synthetic 1-amino acid solution and a commercial fat emulsion. Ann Surg. 1970 Aug;172(2):291–301. doi: 10.1097/00000658-197008000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowers R. E., Brigham K. L., Owen P. J. Salicylate pulmonary edema: the mechanism in sheep and review of the clinical literature. Am Rev Respir Dis. 1977 Feb;115(2):261–268. doi: 10.1164/arrd.1977.115.2.261. [DOI] [PubMed] [Google Scholar]
  5. Brigham K. L., Bowers R. E., Owen P. J. Effects of antihistamines on the lung vascular response to histamine in unanesthetized sheep. Diphenhydramine prevention of pulmonary edema and increased permeability. J Clin Invest. 1976 Aug;58(2):391–398. doi: 10.1172/JCI108483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brigham K. L., Owen P. J. Increased sheep lung vascular permeability caused by histamine. Circ Res. 1975 Nov;37(5):647–657. doi: 10.1161/01.res.37.5.647. [DOI] [PubMed] [Google Scholar]
  7. Brigham K. L., Owen P. J. Mechanism of the serotonin effect on lung transvascular fluid and protein movement in awake sheep. Circ Res. 1975 Jun;36(6):761–770. doi: 10.1161/01.res.36.6.761. [DOI] [PubMed] [Google Scholar]
  8. Brigham K. L., Woolverton W. C., Blake L. H., Staub N. C. Increased sheep lung vascular permeability caused by pseudomonas bacteremia. J Clin Invest. 1974 Oct;54(4):792–804. doi: 10.1172/JCI107819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CRONE C. THE PERMEABILITY OF CAPILLARIES IN VARIOUS ORGANS AS DETERMINED BY USE OF THE 'INDICATOR DIFFUSION' METHOD. Acta Physiol Scand. 1963 Aug;58:292–305. doi: 10.1111/j.1748-1716.1963.tb02652.x. [DOI] [PubMed] [Google Scholar]
  10. Coran A. G. The long-term total intravenous feeding of infants using peripheral veins. J Pediatr Surg. 1973 Oct;8(5):801–807. doi: 10.1016/0022-3468(73)90424-7. [DOI] [PubMed] [Google Scholar]
  11. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  12. Greene H. L., Hazlett D., Demaree R. Relationship between Intralipid-induced hyperlipemia and pulmonary function. Am J Clin Nutr. 1976 Feb;29(2):127–135. doi: 10.1093/ajcn/29.2.127. [DOI] [PubMed] [Google Scholar]
  13. Harris T. R., Brigham K. L., Rowlett R. D. Pressure, serotonin, and histamine effects on lung multiple-indicator curves in sheep. J Appl Physiol Respir Environ Exerc Physiol. 1978 Feb;44(2):245–253. doi: 10.1152/jappl.1978.44.2.245. [DOI] [PubMed] [Google Scholar]
  14. Harris T. R., Rowlett R. D., Brigham K. L. The identification of pulmonary capillary permeability from multiple-indicator data. Effects of increased capillary pressure and alloxan treatment in the dog. Microvasc Res. 1976 Sep;12(2):177–196. doi: 10.1016/0026-2862(76)90017-0. [DOI] [PubMed] [Google Scholar]
  15. JOYNER C. R., Jr, HORWITZ O., WILLIAMS P. G. The effect of lipemia upon tissue oxygen tension in man. Circulation. 1960 Nov;22:901–907. doi: 10.1161/01.cir.22.5.901. [DOI] [PubMed] [Google Scholar]
  16. Kadowitz P. J., Hyman A. L. Influence of a prostaglandin endoperoxide analogue on the canine pulmonary vascular bed. Circ Res. 1977 Mar;40(3):282–287. doi: 10.1161/01.res.40.3.282. [DOI] [PubMed] [Google Scholar]
  17. LINDSTROEM J. MICROCIRCULATORY EFFECTS OF EMULSIFIED FAT INFUSIONS. Circ Res. 1964 Aug;15:124–130. doi: 10.1161/01.res.15.2.124. [DOI] [PubMed] [Google Scholar]
  18. MacFadyen B. V., Jr, Dudrick S. J., Tagudar E. P., Maynard A. T., Law D. K., Rhoads J. E. Triglyceride and free fatty acid clearances in patients receiving complete parenteral nutrition using a ten per cent soybean oil emulsion. Surg Gynecol Obstet. 1973 Nov;137(5):813–815. [PubMed] [Google Scholar]
  19. Nicolaysen G., Nicolaysen A., Staub N. C. A quantitative radioautographic comparison of albumin concentration in different dized lymph vessels in normal mouse lungs. Microvasc Res. 1975 Sep;10(2):138–152. doi: 10.1016/0026-2862(75)90002-3. [DOI] [PubMed] [Google Scholar]
  20. Piper P., Vane J. The release of prostaglandins from lung and other tissues. Ann N Y Acad Sci. 1971 Apr 30;180:363–385. doi: 10.1111/j.1749-6632.1971.tb53205.x. [DOI] [PubMed] [Google Scholar]
  21. Staub N. C., Bland R. D., Brigham K. L., Demling R., Erdmann A. J., 3rd, Woolverton W. C. Preparation of chronic lung lymph fistulas in sheep. J Surg Res. 1975 Nov;19(5):315–320. doi: 10.1016/0022-4804(75)90056-6. [DOI] [PubMed] [Google Scholar]
  22. Vreim C. E., Snashall P. D., Staub N. C. Protein composition of lung fluids in anesthetized dogs with acute cardiogenic edema. Am J Physiol. 1976 Nov;231(5 Pt 1):1466–1469. doi: 10.1152/ajplegacy.1976.231.5.1466. [DOI] [PubMed] [Google Scholar]
  23. Wicks T. C., Rose J. C., Johnson M., Ramwell P. W., Kot P. A. Vascular responses to arachidonic acid in the perfused canine lung. Circ Res. 1976 Mar;38(3):167–171. doi: 10.1161/01.res.38.3.167. [DOI] [PubMed] [Google Scholar]

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