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. 1978 Jun;61(6):1441–1447. doi: 10.1172/JCI109063

Comparative responses of tracheal spirals and parenchymal strips to histamine and carbachol in vitro.

J M Drazen, M W Schneider
PMCID: PMC372669  PMID: 659606

Abstract

The responses of isolated guinea pig tracheal spirals and parenchymal strips to histamine and carbachol were compared. The parenchymal strip, a 1.5 x 1.5 x 20-mm strip cut from the periphery of the lung, constricted at a lower dose and had a larger maximal response to histamine than to carbachol. In contrast, the response of the tracheal spiral to equimolar doses of histamine or carbachol was the same. The responsiveness of both muscle strips to histamine was decreased by treatment with the H1 receptor antagonist mepyramine (0.1 micrometer), and the response to carbachol was blocked by treatment with atropine (0.1 micrometer). Indomethacin (3 micrometer), cimetidine (1 micrometer), propranolol (10 micrometer), and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) (4 micrometer) did not alter the differential response of the two strips to histamine and carbachol. The differential response of parenchymal strips with many, few, or no conducting airways and blood vessels was identical, suggesting that the contractile element is alveolar duct smooth muscle or alveolar contractile elements. This differential pharmacologic response in vitro is consistent with the in vivo observation that histamine causes more peripheral airway constriction than does acetylcholine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burka J. F., Eyre P. Modulation of the release of SRS-A from bovine lung in vitro by several autonomic and autacoid agents. Int Arch Allergy Appl Immunol. 1976;50(6):664–673. doi: 10.1159/000231545. [DOI] [PubMed] [Google Scholar]
  2. CONSTANTINE J. W. THE SPIRALLY CUT TRACHEAL STRIP PREPARATION. J Pharm Pharmacol. 1965 Jun;17:384–385. doi: 10.1111/j.2042-7158.1965.tb07688.x. [DOI] [PubMed] [Google Scholar]
  3. Colebatch H. J., Olsen C. R., Nadel J. A. Effect of histamine, serotonin, and acetylcholine on the peripheral airways. J Appl Physiol. 1966 Jan;21(1):217–226. doi: 10.1152/jappl.1966.21.1.217. [DOI] [PubMed] [Google Scholar]
  4. Conroy J. D., Toledo A. B. Metachromasia and improved histologic detail with toluidine blue-hematoxylin and eosin. Vet Pathol. 1976;13(1):78–80. doi: 10.1177/030098587601300109. [DOI] [PubMed] [Google Scholar]
  5. Drazen J. M., Austen K. F. Atropine modification of the pulmonary effects of chemical mediators in the guinea pig. J Appl Physiol. 1975 May;38(5):834–838. doi: 10.1152/jappl.1975.38.5.834. [DOI] [PubMed] [Google Scholar]
  6. Fleisch J. H., Calkins P. J. Comparison of drug-induced responses of rabbit trachea and bronchus. J Appl Physiol. 1976 Jul;41(1):62–66. doi: 10.1152/jappl.1976.41.1.62. [DOI] [PubMed] [Google Scholar]
  7. HOLGATE J. A., WARNER B. T. Evaluation of antagonists of histamine, 5-hydroxytryptamine and acetylcholine in the guinea-pig. Br J Pharmacol Chemother. 1960 Dec;15:561–566. doi: 10.1111/j.1476-5381.1960.tb00282.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kadlec O., Masek K., Seferna I. A modulating role of prostaglandins in contractions of the guinea-pig ileum. Br J Pharmacol. 1974 Aug;51(4):565–570. doi: 10.1111/j.1476-5381.1974.tb09675.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kapanci Y., Assimacopoulos A., Irle C., Zwahlen A., Gabbiani G. "Contractile interstitial cells" in pulmonary alveolar septa: a possible regulator of ventilation-perfusion ratio? Ultrastructural, immunofluorescence, and in vitro studies. J Cell Biol. 1974 Feb;60(2):375–392. doi: 10.1083/jcb.60.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kliment V. Similarity and dimensional analysis, evaluation of aerosol deposition in the lungs of laboratory animals and man. Folia Morphol (Praha) 1973;21(1):59–64. [PubMed] [Google Scholar]
  11. Lulich K. M., Mitchell H. W., Sparrow M. P. The cat lung strip as an in vitro preparation of peripheral airways: a comparison of beta-adrenoceptor agonists, autacoids and anaphylactic challenge on the lung strip and trachea. Br J Pharmacol. 1976 Sep;58(1):71–79. doi: 10.1111/j.1476-5381.1976.tb07694.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Orehek J., Douglas J. S., Bouhuys A. Contractile responses of the guinea-pig trachea in vitro: modification by prostaglandin synthesis-inhibiting drugs. J Pharmacol Exp Ther. 1975 Sep;194(3):554–564. [PubMed] [Google Scholar]
  13. Persson G. A., Ekman M. Contractile effects of histamine in large and small respiratory airways. Agents Actions. 1976 Jul;6(4):389–393. doi: 10.1007/BF01973208. [DOI] [PubMed] [Google Scholar]

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