Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1978 Jun;61(6):1482–1487. doi: 10.1172/JCI109068

3-Hydroxyproline content of normal urine.

E Adams, S Ramaswamy, M Lamon
PMCID: PMC372674  PMID: 659611

Abstract

Values for total 3-hydroxyproline and 4-hydroxyproline were obtained from 24-h urine specimens of 18 healthy human subjects of both sexes, whose ages ranged from the first to the sixth decade in age. Urinary 3-hydroxyproline levels, not earlier described to our knowledge, were determined by an isotope-dilution method requiring considerable purification and utilizing the amino acid analyzer for final measurement. 3-Hydroxyproline averaged 3% of the corresponding 4-hydroxyproline in individual urine samples. Like 4-hydroxyproline, 3-hydroxyproline excretion is increased in the second decade, and there is generally good correlation between the two values in individual urines. A hydroxyprolinemic subject excreting greatly elevated 4-hydroxyproline levels did not excrete excessive 3-hydroxyproline, consistent with independent catabolic pathways for the two compounds. 3-Hydroxyproline appears to be selectively excreted relative to 4-hydroxyproline when compared with the probable total body content of each amino acid. Possible explanations are: a more rapid turnover of basement membrane collagen than interstitial collagen or, alternatively, relatively greater resistance to the proteolytic cleavage of peptides containing 3-hydroxyproline.

Full text

PDF
1482

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams E. Metabolism of proline and of hydroxyproline. Int Rev Connect Tissue Res. 1970;5:1–91. doi: 10.1016/b978-0-12-363705-5.50007-5. [DOI] [PubMed] [Google Scholar]
  2. Adams E. Synthesis of peptides and derivatives of 3- and 4- hydroxyproline. Int J Pept Protein Res. 1976;8(5):503–516. doi: 10.1111/j.1399-3011.1976.tb02531.x. [DOI] [PubMed] [Google Scholar]
  3. Adams E. The metabolism of hydroxyproline. Mol Cell Biochem. 1973 Dec 15;2(2):109–119. doi: 10.1007/BF01795466. [DOI] [PubMed] [Google Scholar]
  4. Benoit F. L., Watten R. H. Renal tubular transport of hydroxyproline peptides: evidence for reabsorption and secretion. Metabolism. 1968 Jan;17(1):20–33. doi: 10.1016/s0026-0495(68)80004-6. [DOI] [PubMed] [Google Scholar]
  5. Cleary J., Saunders R. A. A simplified procedure for the measurement of total hydroxyproline in urine. Clin Chim Acta. 1974 Dec 17;57(3):217–223. doi: 10.1016/0009-8981(74)90400-8. [DOI] [PubMed] [Google Scholar]
  6. EFRON M. L., BIXBY E. M., PRYLES C. V. HYDROXYPROLINEMIA. II. A RARE METABOLIC DISEASE DUE TO A DEFICIENCY OF THE ENZYME "HYDROXYPROLINE OXIDASE". N Engl J Med. 1965 Jun 24;272:1299–1309. doi: 10.1056/NEJM196506242722501. [DOI] [PubMed] [Google Scholar]
  7. Efron M. L., Bixby E. M., Hockaday T. D., Smith L. H., Jr, Meshorer E. Hydroxyprolinemia. 3. The origin of free hydroxyproline in hydroxyprolinemia. Collagen turnover. Evidence for biosynthetic pathway in man. Biochim Biophys Acta. 1968 Sep 3;165(2):238–250. doi: 10.1016/0304-4165(68)90052-4. [DOI] [PubMed] [Google Scholar]
  8. Gryder R. M., Lamon M., Adams E. Sequence position of 3-hydroxyproline in basement membrane collagen. Isolation of glycyl-3-hydroxyprolyl-4-hydroxyproline from swine kidney. J Biol Chem. 1975 Apr 10;250(7):2470–2474. [PubMed] [Google Scholar]
  9. HAMILTON P. B., ORTIZ P. J. Proline and hydroxyproline; determination of the sum of their alpha-nitrogen. J Biol Chem. 1950 Dec;187(2):733–742. [PubMed] [Google Scholar]
  10. JONES C. R., BERGMAN M. W., KITTNER P. J., PIGMAN W. W. URINARY HYDROXYPROLINE EXCRETION IN NORMAL CHILDREN AND ADOLESCENTS. Proc Soc Exp Biol Med. 1964 Jan;115:85–87. doi: 10.3181/00379727-115-28837. [DOI] [PubMed] [Google Scholar]
  11. Kefalides N. A. Structure and biosynthesis of basement membranes. Int Rev Connect Tissue Res. 1973;6:63–104. doi: 10.1016/b978-0-12-363706-2.50008-8. [DOI] [PubMed] [Google Scholar]
  12. Kivirikko K. I. Urinary excretion of hydroxyproline in health and disease. Int Rev Connect Tissue Res. 1970;5:93–163. doi: 10.1016/b978-0-12-363705-5.50008-7. [DOI] [PubMed] [Google Scholar]
  13. Man M., Adams E. Basement membrane and interstitial collagen content of whole animals and tissues. Biochem Biophys Res Commun. 1975 Sep 2;66(1):9–16. doi: 10.1016/s0006-291x(75)80287-7. [DOI] [PubMed] [Google Scholar]
  14. Miller E. J. Biochemical characteristics and biological significance of the genetically-distinct collagens. Mol Cell Biochem. 1976 Dec 10;13(3):165–192. doi: 10.1007/BF01731779. [DOI] [PubMed] [Google Scholar]
  15. OGLE J. D., ARLINGHAUS R. B., LGAN M. A. 3-Hydroxyproline, a new amino acid of collagen. J Biol Chem. 1962 Dec;237:3667–3673. [PubMed] [Google Scholar]
  16. OGLE J. D., ARLINGHAUS R. B., LOGAN M. A. Studies on peptides obtained from enzymic digests of collagen with evidence for the presence of an unidentified compound in this protein. Arch Biochem Biophys. 1961 Jul;94:85–93. doi: 10.1016/0003-9861(61)90014-5. [DOI] [PubMed] [Google Scholar]
  17. PROCKOP D. J., KEISER H. R., SJOERDSMA A. Gastrointestinal absorption and renal excretion of hydroxyproline peptides. Lancet. 1962 Sep 15;2(7255):527–528. doi: 10.1016/s0140-6736(62)90400-2. [DOI] [PubMed] [Google Scholar]
  18. PROCKOP D. J., UDENFRIEND S. A specific method for the analysis of hydroxyproline in tissues and urine. Anal Biochem. 1960 Nov;1:228–239. doi: 10.1016/0003-2697(60)90050-6. [DOI] [PubMed] [Google Scholar]
  19. Saleh A. E., Coenegracht J. M. The influence of age and weight on the urinary excretion of hydroxyproline and calcium. Clin Chim Acta. 1968 Sep;21(3):445–452. doi: 10.1016/0009-8981(68)90075-2. [DOI] [PubMed] [Google Scholar]
  20. Weiss P. H., Klein L. The quantitative relationship of urinary peptide hydroxyproline excretion to collagen degradation. J Clin Invest. 1969 Jan;48(1):1–10. doi: 10.1172/JCI105957. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES