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. 1978 Jun;61(6):1666–1670. doi: 10.1172/JCI109087

Spironolactone. An aldosterone agonist in the stimulation of H+ secretion by turtle urinary bladder.

A Mueller, P R Steinmetz
PMCID: PMC372693  PMID: 659620

Abstract

In the isolated turtle bladder, spironolactone inhibits sodium transport in the presence of aldosterone or endogenous mineralocorticoid hormone. In contrast to this antagonism for the stimulation of sodium transport by aldosterone, the stimulation of hydrogen ion secretion by aldosterone is not inhibited by spironolactone. In hormone-depleted bladders, spironolactone stimulates hydrogen ion secretion. The extent of stimulation is similar to that of aldosterone. Spironolactone functions as an agonist for aldosterone for the stimulation of urinary acidification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Awqati Q. Effect of aldosterone on the coupling between H+ transport and glucose oxidation. J Clin Invest. 1977 Dec;60(6):1240–1247. doi: 10.1172/JCI108883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Al-Awqati Q., Norby L. H., Mueller A., Steinmetz P. R. Characteristics of stimulation of H+ transport by aldosterone in turtle urinary bladder. J Clin Invest. 1976 Aug;58(2):351–358. doi: 10.1172/JCI108479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRABBE J. DECREASED EFFECTIVENESS OF ALDOSTERONE ON ACTIVE SODIUM TRANSPORT BY THE ISOLATED TOAD BLADDER IN THE PRESENCE OF OTHER STEROIDS. Acta Endocrinol (Copenh) 1964 Nov;47:419–432. doi: 10.1530/acta.0.0470419. [DOI] [PubMed] [Google Scholar]
  4. Feldman D., Funder J. W., Edelman I. S. Subcellular mechanisms in the action of adrenal steroids. Am J Med. 1972 Nov;53(5):545–560. doi: 10.1016/0002-9343(72)90152-0. [DOI] [PubMed] [Google Scholar]
  5. Funder J. W., Feldman D., Highland E., Edelman I. S. Molecular modifications of anti-aldosterone compounds: effects on affinity of spirolactones for renal aldosterone receptors. Biochem Pharmacol. 1974 May 15;23(10):1493–1501. doi: 10.1016/0006-2952(74)90386-4. [DOI] [PubMed] [Google Scholar]
  6. Goodman D. B., Allen J. E., Rasmussen H. Studies on the mechanism of action of aldosterone: hormone-induced changes in lipid metabolism. Biochemistry. 1971 Oct 12;10(21):3825–3831. doi: 10.1021/bi00797a004. [DOI] [PubMed] [Google Scholar]
  7. KAGAWA C. M., CELLA J. A., VAN ARMAN C. G. Action of new steroids in blocking effects of aldosterone and desoxycorticosterone on salt. Science. 1957 Nov 15;126(3281):1015–1016. doi: 10.1126/science.126.3281.1015. [DOI] [PubMed] [Google Scholar]
  8. LeFevre M. E. Effects of aldosterone on the isolated substrate-depleted turtle bladder. Am J Physiol. 1973 Nov;225(5):1252–1256. doi: 10.1152/ajplegacy.1973.225.5.1252. [DOI] [PubMed] [Google Scholar]
  9. Ludens J. H., Fanestil D. D. Aldosterone stimulation of acidification of urine by isolated urinary bladder of the Colombian toad. Am J Physiol. 1974 Jun;226(6):1321–1326. doi: 10.1152/ajplegacy.1974.226.6.1321. [DOI] [PubMed] [Google Scholar]
  10. Marver D., Stewart J., Funder J. W., Feldman D., Edelman I. S. Renal aldosterone receptors: studies with (3H)aldosterone and the anti-mineralocorticoid (3H)spirolactone (SC-26304). Proc Natl Acad Sci U S A. 1974 Apr;71(4):1431–1435. doi: 10.1073/pnas.71.4.1431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Porter G. A. In vitro inhibition of aldosterone-stimulated sodium transport by steroidal spirolactones. Mol Pharmacol. 1968 May;4(3):224–237. [PubMed] [Google Scholar]
  12. Rossier B. C., Wilce P. A., Edelman I. S. Spironolactone antagonism of aldosterone action on Na+ transport and RNA metabolism in toad bladder epithelium. J Membr Biol. 1977 Apr 7;32(1-2):177–194. doi: 10.1007/BF01905216. [DOI] [PubMed] [Google Scholar]
  13. Sakauye C., Feldman D. Agonist and antimineralocorticoid activities of spirolactones. Am J Physiol. 1976 Jul;231(1):93–97. doi: 10.1152/ajplegacy.1976.231.1.93. [DOI] [PubMed] [Google Scholar]
  14. Schwartz J. H., Steinmetz P. R. CO2 requirements for H+ secretion by the isolated turtle bladder. Am J Physiol. 1971 Jun;220(6):2051–2057. doi: 10.1152/ajplegacy.1971.220.6.2051. [DOI] [PubMed] [Google Scholar]
  15. Sharp G. W., Komack C. L., Leaf A. Studies on the binding of aldosterone in the toad bladder. J Clin Invest. 1966 Apr;45(4):450–459. doi: 10.1172/JCI105359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sharp G. W., Leaf A. Mechanism of action of aldosterone. Physiol Rev. 1966 Oct;46(4):593–633. doi: 10.1152/physrev.1966.46.4.593. [DOI] [PubMed] [Google Scholar]
  17. Steinmetz P. R. Cellular mechanisms of urinary acidification. Physiol Rev. 1974 Oct;54(4):890–956. doi: 10.1152/physrev.1974.54.4.890. [DOI] [PubMed] [Google Scholar]
  18. Steinmetz P. R. Characteristics of hydrogen ion transport in urinary bladder of water turtle. J Clin Invest. 1967 Oct;46(10):1531–1540. doi: 10.1172/JCI105644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steinmetz P. R., Omachi R. S., Frazier H. S. Independence of hydrogen ion secretion and transport of other electrolytes in turtle bladder. J Clin Invest. 1967 Oct;46(10):1541–1548. doi: 10.1172/JCI105645. [DOI] [PMC free article] [PubMed] [Google Scholar]

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