Cycling conditions were: an initial 94oC, 3 minute denaturation step; 30 cycles of 94oC for 30s, 60oC for 45s, and 72oC for 60s; and a final 2 minute extension at 72oC.
Footnotes
Competing Interests: No competing interests declared.
Cycling conditions were: an initial 94oC, 3 minute denaturation step; 30 cycles of 94oC for 30s, 60oC for 45s, and 72oC for 60s; and a final 2 minute extension at 72oC.
Competing Interests: No competing interests declared.