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. 1985 Jun;49(2):107–125. doi: 10.1128/mr.49.2.107-125.1985

Molecular biology of trypanosome antigenic variation.

J E Donelson, A C Rice-Ficht
PMCID: PMC373025  PMID: 2409439

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen G., Gurnett L. P., Cross G. A. Complete amino acids sequence of a variant surface glycoprotein (VSG 117) from Trypanosoma brucei. J Mol Biol. 1982 May 25;157(3):527–546. doi: 10.1016/0022-2836(82)90474-0. [DOI] [PubMed] [Google Scholar]
  2. Auffret C. A., Turner M. J. Variant specific antigens of Trypanosoma brucei exist in solution as glycoprotein dimers. Biochem J. 1981 Feb 1;193(2):647–650. doi: 10.1042/bj1930647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltz T., Duvillier G., Giroud C., Richet C., Baltz D., Degand P. The variant surface glycoproteins of Trypanosoma equiperdum. Identification of a phosphorylated glycopeptide as the cross-reacting antigenic determinant. FEBS Lett. 1983 Jul 11;158(1):174–178. doi: 10.1016/0014-5793(83)80702-9. [DOI] [PubMed] [Google Scholar]
  4. Baltz T., Giroud C., Baltz D., Duvillier G., Degand P., Demaille J., Pautrizel R. The variable surface glycoproteins of Trypanosoma equiperdum are phosphorylated. EMBO J. 1982;1(11):1393–1398. doi: 10.1002/j.1460-2075.1982.tb01328.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barbet A. F., Davis W. C., McGuire T. C. Cross-neutralization of two different trypanosome populations derived from a single organism. Nature. 1982 Dec 2;300(5891):453–456. doi: 10.1038/300453a0. [DOI] [PubMed] [Google Scholar]
  6. Barbet A. F., McGuire T. C. Crossreacting determinants in variant-specific surface antigens of African trypanosomes. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1989–1993. doi: 10.1073/pnas.75.4.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Barry J. D., Crowe J. S., Vickerman K. Instability of the Trypanosoma brucei rhodesiense metacyclic variable antigen repertoire. Nature. 1983 Dec 15;306(5944):699–701. doi: 10.1038/306699a0. [DOI] [PubMed] [Google Scholar]
  8. Bernards A., De Lange T., Michels P. A., Liu A. Y., Huisman M. J., Borst P. Two modes of activation of a single surface antigen gene of Trypanosoma brucei. Cell. 1984 Jan;36(1):163–170. doi: 10.1016/0092-8674(84)90085-0. [DOI] [PubMed] [Google Scholar]
  9. Bernards A., Michels P. A., Lincke C. R., Borst P. Growth of chromosome ends in multiplying trypanosomes. Nature. 1983 Jun 16;303(5918):592–597. doi: 10.1038/303592a0. [DOI] [PubMed] [Google Scholar]
  10. Bernards A., Van der Ploeg L. H., Frasch A. C., Borst P., Boothroyd J. C., Coleman S., Cross G. A. Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3' end. Cell. 1981 Dec;27(3 Pt 2):497–505. doi: 10.1016/0092-8674(81)90391-3. [DOI] [PubMed] [Google Scholar]
  11. Bernards A., van Harten-Loosbroek N., Borst P. Modification of telomeric DNA in Trypanosoma brucei; a role in antigenic variation? Nucleic Acids Res. 1984 May 25;12(10):4153–4170. doi: 10.1093/nar/12.10.4153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Beverley S. M., Coderre J. A., Santi D. V., Schimke R. T. Unstable DNA amplifications in methotrexate-resistant Leishmania consist of extrachromosomal circles which relocalize during stabilization. Cell. 1984 Sep;38(2):431–439. doi: 10.1016/0092-8674(84)90498-7. [DOI] [PubMed] [Google Scholar]
  13. Bienen E. J., Hammadi E., Hill G. C. Trypanosoma brucei: biochemical and morphological changes during in vitro transformation of bloodstream- to procyclic-trypomastigotes. Exp Parasitol. 1981 Jun;51(3):408–417. doi: 10.1016/0014-4894(81)90128-4. [DOI] [PubMed] [Google Scholar]
  14. Blackburn E. H., Challoner P. B. Identification of a telomeric DNA sequence in Trypanosoma brucei. Cell. 1984 Feb;36(2):447–457. doi: 10.1016/0092-8674(84)90238-1. [DOI] [PubMed] [Google Scholar]
  15. Bogucki M. S., Onodera M., Rosen N. L., Lifter J., Hotez P. J., Konigsberg W. H., Richards F. F. Trypanosoma congolense: surface glycoproteins of two early bloodstream variants. III. Immunochemical characterization. Exp Parasitol. 1982 Feb;53(1):1–10. doi: 10.1016/0014-4894(82)90086-8. [DOI] [PubMed] [Google Scholar]
  16. Boothroyd J. C., Cross G. A., Hoeijmakers J. H., Borst P. A variant surface glycoprotein of Trypanosoma brucei synthesized with a C-terminal hydrophobic 'tail' absent from purified glycoprotein. Nature. 1980 Dec 11;288(5791):624–626. doi: 10.1038/288624a0. [DOI] [PubMed] [Google Scholar]
  17. Boothroyd J. C., Paynter C. A., Cross G. A., Bernards A., Borst P. Variant surface glycoproteins of Trypanosoma brucei are synthesised with cleavable hydrophobic sequences at the carboxy and amino termini. Nucleic Acids Res. 1981 Sep 25;9(18):4735–4743. doi: 10.1093/nar/9.18.4735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Borst P., Bernards A., van der Ploeg L. H., Michels P. A., Liu A. Y., de Lange T., Kooter J. M. The control of variant surface antigen synthesis in trypanosomes. Eur J Biochem. 1983 Dec 15;137(3):383–389. doi: 10.1111/j.1432-1033.1983.tb07840.x. [DOI] [PubMed] [Google Scholar]
  19. Borst P., Cross G. A. Molecular basis for trypanosome antigenic variation. Cell. 1982 Jun;29(2):291–303. doi: 10.1016/0092-8674(82)90146-5. [DOI] [PubMed] [Google Scholar]
  20. Borst P., Frasch A. C., Bernards A., Hoeijmakers J. H., Van der Ploeg L. H., Cross G. A. The genes for variant antigens in trypanosomes. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1033–1036. doi: 10.4269/ajtmh.1980.29.1033. [DOI] [PubMed] [Google Scholar]
  21. Borst P., Frasch A. C., Bernards A., Van der Ploeg L. H., Hoeijmakers J. H., Arnberg A. C., Cross G. A. DNA rearrangements involving the genes for variant antigens in Trypanosoma brucei. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):935–943. doi: 10.1101/sqb.1981.045.01.110. [DOI] [PubMed] [Google Scholar]
  22. Borst P., van der Ploeg M., van Hoek J. F., Tas J., James J. On the DNA content and ploidy of trypanosomes. Mol Biochem Parasitol. 1982 Jul;6(1):13–23. doi: 10.1016/0166-6851(82)90049-4. [DOI] [PubMed] [Google Scholar]
  23. Bridgen P. J., Cross G. A., Bridgen J. N-terminal amino acid sequences of variant-specific surface antigens from Trypanosoma brucei. Nature. 1976 Oct 14;263(5578):613–614. doi: 10.1038/263613a0. [DOI] [PubMed] [Google Scholar]
  24. Buck G. A., Longacre S., Raibaud A., Hibner U., Giroud C., Baltz T., Baltz D., Eisen H. Stability of expression-linked surface antigen gene in Trypanosoma equiperdum. Nature. 1984 Feb 9;307(5951):563–566. doi: 10.1038/307563a0. [DOI] [PubMed] [Google Scholar]
  25. Campbell D. A., Thornton D. A., Boothroyd J. C. Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genes. 1984 Sep 27-Oct 3Nature. 311(5984):350–355. doi: 10.1038/311350a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Campbell D. A., van Bree M. P., Boothroyd J. C. The 5'-limit of transposition and upstream barren region of a trypanosome VSG gene: tandem 76 base-pair repeats flanking (TAA)90. Nucleic Acids Res. 1984 Mar 26;12(6):2759–2774. doi: 10.1093/nar/12.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Campbell G. H., Esser K. M., Wellde B. T., Diggs C. L. Isolation and characterization of a new serodeme of Trypanosoma rhodesiense. Am J Trop Med Hyg. 1979 Nov;28(6):974–983. doi: 10.4269/ajtmh.1979.28.974. [DOI] [PubMed] [Google Scholar]
  28. Capbern A., Giroud C., Baltz T., Mattern P. Trypanosoma equiperdum: etude des variations antigéniques au cours de la trypanosomose experimentale du lapin. Exp Parasitol. 1977 Jun;42(1):6–13. doi: 10.1016/0014-4894(77)90055-8. [DOI] [PubMed] [Google Scholar]
  29. Cardoso de Almeida M. L., Turner M. J. The membrane form of variant surface glycoproteins of Trypanosoma brucei. Nature. 1983 Mar 24;302(5906):349–352. doi: 10.1038/302349a0. [DOI] [PubMed] [Google Scholar]
  30. Coderre J. A., Beverley S. M., Schimke R. T., Santi D. V. Overproduction of a bifunctional thymidylate synthetase-dihydrofolate reductase and DNA amplification in methotrexate-resistant Leishmania tropica. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2132–2136. doi: 10.1073/pnas.80.8.2132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Cohen C., Reinhardt B., Parry D. A., Roelants G. E., Hirsch W., Kanwé B. Alpha-helical coiled-coil structures of Trypanosoma brucei variable surface glycoproteins. Nature. 1984 Sep 13;311(5982):169–171. doi: 10.1038/311169a0. [DOI] [PubMed] [Google Scholar]
  32. Cross G. A. Antigenic variation in trypanosomes. Proc R Soc Lond B Biol Sci. 1978 Jun 5;202(1146):55–72. doi: 10.1098/rspb.1978.0057. [DOI] [PubMed] [Google Scholar]
  33. Cross G. A. Crossreacting determinants in the C-terminal region of trypanosome variant surface antigens. Nature. 1979 Jan 25;277(5694):310–312. doi: 10.1038/277310a0. [DOI] [PubMed] [Google Scholar]
  34. Cross G. A., Holder A. A., Allen G., Boothroyd J. C. An introduction to antigenic variation in trypanosomes. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1027–1032. doi: 10.4269/ajtmh.1980.29.1027. [DOI] [PubMed] [Google Scholar]
  35. Cross G. A. Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology. 1975 Dec;71(3):393–417. doi: 10.1017/s003118200004717x. [DOI] [PubMed] [Google Scholar]
  36. Crowe J. S., Barry J. D., Luckins A. G., Ross C. A., Vickerman K. All metacyclic variable antigen types of Trypanosoma congolense identified using monoclonal antibodies. Nature. 1983 Nov 24;306(5941):389–391. doi: 10.1038/306389a0. [DOI] [PubMed] [Google Scholar]
  37. De Lange T., Berkvens T. M., Veerman H. J., Frasch A. C., Barry J. D., Borst P. Comparison of the genes coding for the common 5' terminal sequence of messenger RNAs in three trypanosome species. Nucleic Acids Res. 1984 Jun 11;12(11):4431–4443. doi: 10.1093/nar/12.11.4431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. De Lange T., Borst P. Genomic environment of the expression-linked extra copies of genes for surface antigens of Trypanosoma brucei resembles the end of a chromosome. Nature. 1982 Sep 30;299(5882):451–453. doi: 10.1038/299451a0. [DOI] [PubMed] [Google Scholar]
  39. De Lange T., Kooter J. M., Michels P. A., Borst P. Telomere conversion in trypanosomes. Nucleic Acids Res. 1983 Dec 10;11(23):8149–8165. doi: 10.1093/nar/11.23.8149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. De Lange T., Liu A. Y., Van der Ploeg L. H., Borst P., Tromp M. C., Van Boom J. H. Tandem repetition of the 5' mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription? Cell. 1983 Oct;34(3):891–900. doi: 10.1016/0092-8674(83)90546-9. [DOI] [PubMed] [Google Scholar]
  41. De Lange T., Michels P. A., Veerman H. J., Cornelissen A. W., Borst P. Many trypanosome messenger RNAs share a common 5' terminal sequence. Nucleic Acids Res. 1984 May 11;12(9):3777–3790. doi: 10.1093/nar/12.9.3777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Donelson J. E., Murphy W. J., Brentano S. T., Rice-Ficht A. C., Cain G. D. Comparison of the expression-linked extra copy (ELC) and basic copy (BC) genes of a trypanosome surface antigen. J Cell Biochem. 1983;23(1-4):1–12. doi: 10.1002/jcb.240230102. [DOI] [PubMed] [Google Scholar]
  43. Donelson J. E., Young J. R., Dorfman D., Majiwa P. A., Williams R. O. The ILtat 1.4 surface antigen gene family of Trypanosoma brucei. Nucleic Acids Res. 1982 Nov 11;10(21):6581–6595. doi: 10.1093/nar/10.21.6581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Donelson J. E., Young J. R., Dorfman D., Majiwa P. A., Williams R. O. The ILtat 1.4 surface antigen gene family of Trypanosoma brucei. Nucleic Acids Res. 1982 Nov 11;10(21):6581–6595. doi: 10.1093/nar/10.21.6581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Dorfman D. M., Donelson J. E. Characterization of the 1.35 kilobase DNA repeat unit containing the conserved 35 nucleotides at the 5'-termini of variable surface glycoprotein mRNAs in Trypanosoma brucei. Nucleic Acids Res. 1984 Jun 25;12(12):4907–4920. doi: 10.1093/nar/12.12.4907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Doyle J. J., Hirumi H., Hirumi K., Lupton E. N., Cross G. A. Antigenic variation in clones of animal-infective Trypanosoma brucei derived and maintained in vitro. Parasitology. 1980 Apr;80(2):359–369. doi: 10.1017/s0031182000000810. [DOI] [PubMed] [Google Scholar]
  47. Duvillier G., Aubert J. P., Baltz T., Richet C., Degand P. Variant specific surface antigens from Trypanosoma equiperdum: chemical and physical studies. Biochem Biophys Res Commun. 1983 Jan 27;110(2):491–498. doi: 10.1016/0006-291x(83)91176-2. [DOI] [PubMed] [Google Scholar]
  48. Duvillier G., Nouvelot A., Richet C., Baltz T., Degand P. Presence of glycerol and fatty acids in the C-terminal end of a variant surface glycoprotein from Trypanosoma equiperdum. Biochem Biophys Res Commun. 1983 Jul 18;114(1):119–125. doi: 10.1016/0006-291x(83)91602-9. [DOI] [PubMed] [Google Scholar]
  49. Englund P. T., Hajduk S. L., Marini J. C. The molecular biology of trypanosomes. Annu Rev Biochem. 1982;51:695–726. doi: 10.1146/annurev.bi.51.070182.003403. [DOI] [PubMed] [Google Scholar]
  50. Esser K. M., Schoenbechler M. J., Gingrich J. B. Trypanosoma rhodesiense blood forms express all antigen specificities relevant to protection against metacyclic (insect form) challenge. J Immunol. 1982 Oct;129(4):1715–1718. [PubMed] [Google Scholar]
  51. Ferguson M. A., Cross G. A. Myristylation of the membrane form of a Trypanosoma brucei variant surface glycoprotein. J Biol Chem. 1984 Mar 10;259(5):3011–3015. [PubMed] [Google Scholar]
  52. Fink G. R., Petes T. D. Gene conversion in the absence of reciprocal recombination. 1984 Aug 30-Sep 5Nature. 310(5980):728–729. doi: 10.1038/310728a0. [DOI] [PubMed] [Google Scholar]
  53. Frasch A. C., Borst P., Van den Burg J. Rapid evolution of genes coding for variant surface glycoproteins in trypanosomes. Gene. 1982 Feb;17(2):197–211. doi: 10.1016/0378-1119(82)90073-7. [DOI] [PubMed] [Google Scholar]
  54. Freymann D. M., Metcalf P., Turner M., Wiley D. C. 6 A-resolution X-ray structure of a variable surface glycoprotein from Trypanosoma brucei. Nature. 1984 Sep 13;311(5982):167–169. doi: 10.1038/311167a0. [DOI] [PubMed] [Google Scholar]
  55. Gardiner P. R., Finerty J. F., Dwyer D. M. Iodination and identification of surface membrane antigens in procyclic Trypanosoma rhodesiense. J Immunol. 1983 Jul;131(1):454–457. [PubMed] [Google Scholar]
  56. Gray A. R. Antigenic variation in a strain of Trypanosoma brucei transmitted by Glossina morsitans and G. palpalis. J Gen Microbiol. 1965 Nov;41(2):195–214. doi: 10.1099/00221287-41-2-195. [DOI] [PubMed] [Google Scholar]
  57. Hajduk S. L. Antigenic variation during the developmental cycle of Trypanosoma brucei. J Protozool. 1984 Feb;31(1):41–47. [PubMed] [Google Scholar]
  58. Hasan G., Turner M. J., Cordingley J. S. Complete nucleotide sequence of an unusual mobile element from trypanosoma brucei. Cell. 1984 May;37(1):333–341. doi: 10.1016/0092-8674(84)90329-5. [DOI] [PubMed] [Google Scholar]
  59. Hoeijmakers J. H., Frasch A. C., Bernards A., Borst P., Cross G. A. Novel expression-linked copies of the genes for variant surface antigens in trypanosomes. Nature. 1980 Mar 6;284(5751):78–80. doi: 10.1038/284078a0. [DOI] [PubMed] [Google Scholar]
  60. Holder A. A. Carbohydrate is linked through ethanolamine to the C-terminal amino acid of Trypanosoma brucei variant surface glycoprotein. Biochem J. 1983 Jan 1;209(1):261–262. doi: 10.1042/bj2090261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Holder A. A., Cross G. A. Glycopeptides from variant surface glycoproteins of Trypanosoma Brucei. C-terminal location of antigenically cross-reacting carbohydrate moieties. Mol Biochem Parasitol. 1981 Feb;2(3-4):135–150. doi: 10.1016/0166-6851(81)90095-5. [DOI] [PubMed] [Google Scholar]
  62. Johnson J. G., Cross G. A. Selective cleavage of variant surface glycoproteins from Trypanosoma brucei. Biochem J. 1979 Mar 15;178(3):689–697. doi: 10.1042/bj1780689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Kooter J. M., De Lange T., Borst P. Discontinuous synthesis of mRNA in trypanosomes. EMBO J. 1984 Oct;3(10):2387–2392. doi: 10.1002/j.1460-2075.1984.tb02144.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Labastie M. C., Baltz T., Richet C., Giroud C., Duvillier G., Pautrizel R., Degand P. Variant specific glycoproteins of Trypanosoma equiperdum: cross reacting determinants and chemical studies. Biochem Biophys Res Commun. 1981 Mar 31;99(2):729–736. doi: 10.1016/0006-291x(81)91804-0. [DOI] [PubMed] [Google Scholar]
  65. Lalor T. M., Kjeldgaard M., Shimamoto G. T., Strickler J. E., Konigsberg W. H., Richards F. F. Trypanosome variant-specific glycoproteins: a polygene protein family with multiple folding patterns? Proc Natl Acad Sci U S A. 1984 Feb;81(4):998–1002. doi: 10.1073/pnas.81.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Laurent M., Pays E., Magnus E., Van Meirvenne N., Matthyssens G., Williams R. O., Steinert M. DNA rearrangements linked to expression of a predominant surface antigen gene of trypanosomes. Nature. 1983 Mar 17;302(5905):263–266. doi: 10.1038/302263a0. [DOI] [PubMed] [Google Scholar]
  67. Le Ray D., Barry J. D., Easton C., Vickerman K. First tsetse fly transmission of the "AnTat" serodeme of Trypanosoma brucei. Ann Soc Belg Med Trop. 1977;57(4-5):369–381. [PubMed] [Google Scholar]
  68. Le Ray D., Barry J. D., Vickerman K. Antigenic heterogeneity of metacyclic forms of Trypanosoma brucei. Nature. 1978 May 25;273(5660):300–302. doi: 10.1038/273300a0. [DOI] [PubMed] [Google Scholar]
  69. Lenardo M. J., Rice-Ficht A. C., Kelly G., Esser K. M., Donelson J. E. Characterization of the genes specifying two metacyclic variable antigen types in Trypanosoma brucei rhodesiense. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6642–6646. doi: 10.1073/pnas.81.21.6642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  70. Lheureux M., Lheureux M., Vervoort T., Van Meirvenne N., Steinert M. Immunological purification and partial characterization of variant-specific surface antigen messenger RNA of Trypanosoma brucei brucei. Nucleic Acids Res. 1979 Oct 10;7(3):595–609. doi: 10.1093/nar/7.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  71. Liu A. Y., Van der Ploeg L. H., Rijsewijk F. A., Borst P. The transposition unit of variant surface glycoprotein gene 118 of Trypanosoma brucei. Presence of repeated elements at its border and absence of promoter-associated sequences. J Mol Biol. 1983 Jun 15;167(1):57–75. doi: 10.1016/s0022-2836(83)80034-5. [DOI] [PubMed] [Google Scholar]
  72. Longacre S., Hibner U., Raibaud A., Eisen H., Baltz T., Giroud C., Baltz D. DNA rearrangements and antigenic variation in Trypanosoma equiperdum: multiple expression-linked sites in independent isolates of trypanosomes expressing the same antigen. Mol Cell Biol. 1983 Mar;3(3):399–409. doi: 10.1128/mcb.3.3.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  73. Longacre S., Raibaud A., Hibner U., Buck G., Eisen H., Baltz T., Giroud C., Baltz D. DNA rearrangements and antigenic variation in Trypanosoma equiperdum: expression-independent DNA rearrangements in the basic copy of a variant surface glycoprotein gene. Mol Cell Biol. 1983 Mar;3(3):410–414. doi: 10.1128/mcb.3.3.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Magnus E., Vervoort T., Van Meirvenne N. Serological cross-reactions among trypanosome variable antigen isotypes of the subgenus Trypanozoon. Ann Soc Belg Med Trop. 1982 Mar;62(1):25–39. [PubMed] [Google Scholar]
  75. Majiwa P. A., Young J. R., Englund P. T., Shapiro S. Z., Williams R. O. Two distinct forms of surface antigen gene rearrangement in Trypanosoma brucei. Nature. 1982 Jun 10;297(5866):514–516. doi: 10.1038/297514a0. [DOI] [PubMed] [Google Scholar]
  76. Majumder H. K., Boothroyd J. C., Weber H. Homologous 3'-terminal regions of mRNAs for surface antigens of different antigenic variants of Trypanosoma brucei. Nucleic Acids Res. 1981 Sep 25;9(18):4745–4753. doi: 10.1093/nar/9.18.4745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  77. Matthyssens G., Michiels F., Hamers R., Pays E., Steinert M. Two variant surface glycoproteins of Trypanosoma brucei have a conserved C-terminus. Nature. 1981 Sep 17;293(5829):230–233. doi: 10.1038/293230a0. [DOI] [PubMed] [Google Scholar]
  78. McConnell J., Gurnett A. M., Cordingley J. S., Walker J. E., Turner M. J. Biosynthesis of Trypanosoma brucei variant surface glycoprotein. I. Synthesis, size, and processing of an N-terminal signal peptide. Mol Biochem Parasitol. 1981 Dec;4(3-4):225–242. doi: 10.1016/0166-6851(81)90021-9. [DOI] [PubMed] [Google Scholar]
  79. Merritt S. C., Tschudi C., Konigsberg W. H., Richards F. F. Reverse transcription of trypanosome variable antigen mRNAs initiated by a specific oligonucleotide primer. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1536–1540. doi: 10.1073/pnas.80.6.1536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Michels P. A., Bernards A., Van der Ploeg L. H., Borst P. Characterization of the expression-linked gene copies of variant surface glycoprotein 118 in two independently isolated clones of Trypanosoma brucei. Nucleic Acids Res. 1982 Apr 10;10(7):2353–2366. doi: 10.1093/nar/10.7.2353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  81. Michels P. A., Liu A. Y., Bernards A., Sloof P., Van der Bijl M. M., Schinkel A. H., Menke H. H., Borst P., Veeneman G. H., Tromp M. C. Activation of the genes for variant surface glycoproteins 117 and 118 in Trypanosoma brucei. J Mol Biol. 1983 Jun 5;166(4):537–556. doi: 10.1016/s0022-2836(83)80283-6. [DOI] [PubMed] [Google Scholar]
  82. Michiels F., Matthyssens G., Kronenberger P., Pays E., Dero B., Van Assel S., Darville M., Carvador A., Steinert M., Hamers R. Gene activation and re-expression of a Trypanosoma brucei variant surface glycoprotein. EMBO J. 1983;2(7):1185–1192. doi: 10.1002/j.1460-2075.1983.tb01565.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  83. Milhausen M., Nelson R. G., Parsons M., Newport G., Stuart K., Agabian N. Molecular characterization of initial variants from the IsTat I serodeme of Trypanosoma brucei. Mol Biochem Parasitol. 1983 Nov;9(3):241–254. doi: 10.1016/0166-6851(83)90100-7. [DOI] [PubMed] [Google Scholar]
  84. Milhausen M., Nelson R. G., Sather S., Selkirk M., Agabian N. Identification of a small RNA containing the trypanosome spliced leader: a donor of shared 5' sequences of trypanosomatid mRNAs? Cell. 1984 Oct;38(3):721–729. doi: 10.1016/0092-8674(84)90267-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  85. Miller E. N., Turner M. J. Analysis of antigenic types appearing in first relapse populations of clones of Trypanosoma brucei. Parasitology. 1981 Feb;82(1):63–80. doi: 10.1017/s0031182000041871. [DOI] [PubMed] [Google Scholar]
  86. Murphy W. J., Brentano S. T., Rice-Ficht A. C., Dorfman D. M., Donelson J. E. DNA rearrangements of the variable surface antigen genes of the trypanosomes. J Protozool. 1984 Feb;31(1):65–73. doi: 10.1111/j.1550-7408.1984.tb04291.x. [DOI] [PubMed] [Google Scholar]
  87. Myler P. J., Allison J., Agabian N., Stuart K. Antigenic variation in African trypanosomes by gene replacement or activation of alternate telomeres. Cell. 1984 Nov;39(1):203–211. doi: 10.1016/0092-8674(84)90206-x. [DOI] [PubMed] [Google Scholar]
  88. Myler P., Nelson R. G., Agabian N., Stuart K. Two mechanisms of expression of a predominant variant antigen gene of Trypanosoma brucei. Nature. 1984 May 17;309(5965):282–284. doi: 10.1038/309282a0. [DOI] [PubMed] [Google Scholar]
  89. Nelson R. G., Parsons M., Barr P. J., Stuart K., Selkirk M., Agabian N. Sequences homologous to the variant antigen mRNA spliced leader are located in tandem repeats and variable orphons in trypanosoma brucei. Cell. 1983 Oct;34(3):901–909. doi: 10.1016/0092-8674(83)90547-0. [DOI] [PubMed] [Google Scholar]
  90. Nelson R. G., Parsons M., Selkirk M., Newport G., Barr P. J., Agabian N. Sequences homologous to variant antigen mRNA spliced leader in Trypanosomatidae which do not undergo antigenic variation. Nature. 1984 Apr 12;308(5960):665–667. doi: 10.1038/308665a0. [DOI] [PubMed] [Google Scholar]
  91. Olafson R. W., Clarke M. W., Kielland S. L., Pearson T. W., Barbet A. F., McGuire T. C. Amino terminal sequence homology among variant surface glycoproteins of African trypanosomes. Mol Biochem Parasitol. 1984 Jul;12(3):287–298. doi: 10.1016/0166-6851(84)90085-9. [DOI] [PubMed] [Google Scholar]
  92. Olenick J. G., Travis R. W., Garson S. Trypanosoma rhodesiense: chemical and immunological characterization of variant-specific surface coat glycoproteins. Mol Biochem Parasitol. 1981 Aug;3(4):227–238. doi: 10.1016/0166-6851(81)90054-2. [DOI] [PubMed] [Google Scholar]
  93. Onodera M., Rosen N. L., Lifter J., Hotez P. J., Bogucki M. S., Davis G., Patton C. L., Konigsberg W. H., Richards F. F. Trypanosoma congolense: surface glycoproteins of two early bloodstream variants. II. Purification and partial chemical characterization. Exp Parasitol. 1981 Dec;52(3):427–439. doi: 10.1016/0014-4894(81)90102-8. [DOI] [PubMed] [Google Scholar]
  94. Overath P., Czichos J., Stock U., Nonnengaesser C. Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei. EMBO J. 1983;2(10):1721–1728. doi: 10.1002/j.1460-2075.1983.tb01648.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  95. Parsons M., Nelson R. G., Newport G., Milhausen M., Stuart K., Agabian N. Genomic organization of Trypanosoma brucei variant antigen gene families in sequential parasitemias. Mol Biochem Parasitol. 1983 Nov;9(3):255–269. doi: 10.1016/0166-6851(83)90101-9. [DOI] [PubMed] [Google Scholar]
  96. Parsons M., Nelson R. G., Stuart K., Agabian N. Variant antigen genes of Trypanosoma brucei: genomic alteration of a spliced leader orphon and retention of expression-linked copies during differentiation. Proc Natl Acad Sci U S A. 1984 Feb;81(3):684–688. doi: 10.1073/pnas.81.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  97. Parsons M., Nelson R. G., Watkins K. P., Agabian N. Trypanosome mRNAs share a common 5' spliced leader sequence. Cell. 1984 Aug;38(1):309–316. doi: 10.1016/0092-8674(84)90552-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  98. Pays E., Dekerck P., Van Assel S., Babiker E. A., Le Ray D., Van Meirvenne N., Steinert M. Comparative analysis of a Trypanosoma brucei gambiense antigen gene family and its potential use in epidemiology of sleeping sickness. Mol Biochem Parasitol. 1983 Jan;7(1):63–74. doi: 10.1016/0166-6851(83)90117-2. [DOI] [PubMed] [Google Scholar]
  99. Pays E., Delauw M. F., Laurent M., Steinert M. Possible DNA modification in GC dinucleotides of Trypanosoma brucei telomeric sequences; relationship with antigen gene transcription. Nucleic Acids Res. 1984 Jul 11;12(13):5235–5247. doi: 10.1093/nar/12.13.5235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  100. Pays E., Delauw M. F., Van Assel S., Laurent M., Vervoort T., Van Meirvenne N., Steinert M. Modifications of a Trypanosoma b. brucei antigen gene repertoire by different DNA recombinational mechanisms. Cell. 1983 Dec;35(3 Pt 2):721–731. doi: 10.1016/0092-8674(83)90105-8. [DOI] [PubMed] [Google Scholar]
  101. Pays E., Delronche M., Lheureux M., Vervoort T., Bloch J., Gannon F., Steinert M. Cloning and characterization of DNA sequences complementary to messenger ribonucleic acids coding for the synthesis of two surface antigens of Trypanosoma brucei. Nucleic Acids Res. 1980 Dec 20;8(24):5965–5981. doi: 10.1093/nar/8.24.5965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  102. Pays E., Laurent M., Delinte K., Van Meirvenne N., Steinert M. Differential size variations between transcriptionally active and inactive telomeres of Trypanosoma brucei. Nucleic Acids Res. 1983 Dec 10;11(23):8137–8147. doi: 10.1093/nar/11.23.8137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  103. Pays E., Lheureux M., Steinert M. Analysis of the DNA and RNA changes associated with the expression of isotypic variant-specific antigens of trypanosomes. Nucleic Acids Res. 1981 Sep 11;9(17):4225–4238. doi: 10.1093/nar/9.17.4225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  104. Pays E., Lheureux M., Steinert M. Structure and expression of a Trypanosoma brucei gambiense variant specific antigen gene. Nucleic Acids Res. 1982 May 25;10(10):3149–3163. doi: 10.1093/nar/10.10.3149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  105. Pays E., Lheureux M., Vervoort T., Steinert M. Conservation of a variant-specific surface antigen gene in different trypanosome species and sub-species. Mol Biochem Parasitol. 1981 Dec 31;4(5-6):349–357. doi: 10.1016/0166-6851(81)90066-9. [DOI] [PubMed] [Google Scholar]
  106. Pays E., Van Assel S., Laurent M., Darville M., Vervoort T., Van Meirvenne N., Steinert M. Gene conversion as a mechanism for antigenic variation in trypanosomes. Cell. 1983 Sep;34(2):371–381. doi: 10.1016/0092-8674(83)90371-9. [DOI] [PubMed] [Google Scholar]
  107. Pays E., Van Assel S., Laurent M., Dero B., Michiels F., Kronenberger P., Matthyssens G., Van Meirvenne N., Le Ray D., Steinert M. At least two transposed sequences are associated in the expression site of a surface antigen gene in different trypanosome clones. Cell. 1983 Sep;34(2):359–369. doi: 10.1016/0092-8674(83)90370-7. [DOI] [PubMed] [Google Scholar]
  108. Pays E., Van Meirvenne N., Le Ray D., Steinert M. Gene duplication and transposition linked to antigenic variation in Trypanosoma brucei. Proc Natl Acad Sci U S A. 1981 May;78(5):2673–2677. doi: 10.1073/pnas.78.5.2673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  109. Penncavage N. A., Julius M. A., Marcu K. B. An African trypanosome variant surface glycoprotein gene whose expression is not activated by duplication. Nucleic Acids Res. 1983 Dec 10;11(23):8343–8357. doi: 10.1093/nar/11.23.8343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  110. Raibaud A., Gaillard C., Longacre S., Hibner U., Buck G., Bernardi G., Eisen H. Genomic environment of variant surface antigen genes of Trypanosoma equiperdum. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4306–4310. doi: 10.1073/pnas.80.14.4306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  111. Rautenberg P., Reinwald E., Risse H. J. Sialic acids are responsible for charge heterogeneity of the variant surface glycoprotein of Trypanosoma congolense. Mol Biochem Parasitol. 1981 Dec;4(3-4):129–138. doi: 10.1016/0166-6851(81)90012-8. [DOI] [PubMed] [Google Scholar]
  112. Reinwald E., Rautenberg P., Risse H. J. Purification of the variant antigens of Trypanosoma congolense: a new approach to the isolation of glycoproteins. Biochim Biophys Acta. 1981 Mar 27;668(1):119–131. doi: 10.1016/0005-2795(81)90155-0. [DOI] [PubMed] [Google Scholar]
  113. Rice-Ficht A. C., Chen K. K., Donelson J. E. Point mutations during generation of expression-linked extra copy of trypanosome surface glycoprotein gene. Nature. 1982 Aug 12;298(5875):676–679. doi: 10.1038/298676a0. [DOI] [PubMed] [Google Scholar]
  114. Richards F. F., Rosen N. L., Onodera M., Bogucki M. S., Neve R. L., Hotez P., Armstrong M. Y., Konigsberg W. H. Antigenic variation and the surface glycoproteins of Trypanosoma congolense. Fed Proc. 1981 Apr;40(5):1434–1439. [PubMed] [Google Scholar]
  115. Richards F. F. The surface of the African trypanosomes. J Protozool. 1984 Feb;31(1):60–64. doi: 10.1111/j.1550-7408.1984.tb04290.x. [DOI] [PubMed] [Google Scholar]
  116. Rifkin M. R. Identification of the trypanocidal factor in normal human serum: high density lipoprotein. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3450–3454. doi: 10.1073/pnas.75.7.3450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  117. Roberstson M. Gene rearrangement and the generation of diversity. Nature. 1982 May 20;297(5863):184–186. doi: 10.1038/297184a0. [DOI] [PubMed] [Google Scholar]
  118. Savage A., Geyer R., Stirm S., Reinwald E., Risse H. J. Structural studies on the major oligosaccharides in a variant surface glycoprotein of Trypanosoma congolense. Mol Biochem Parasitol. 1984 Apr;11:309–328. doi: 10.1016/0166-6851(84)90075-6. [DOI] [PubMed] [Google Scholar]
  119. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  120. Sloof P., Bos J. L., Konings A. F., Menke H. H., Borst P., Gutteridge W. E., Leon W. Characterization of satellite DNA in Trypanosoma brucei and Trypanosoma cruzi. J Mol Biol. 1983 Jun 15;167(1):1–21. doi: 10.1016/s0022-2836(83)80031-x. [DOI] [PubMed] [Google Scholar]
  121. Sloof P., Menke H. H., Caspers M. P., Borst P. Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction. Nucleic Acids Res. 1983 Jun 25;11(12):3889–3901. doi: 10.1093/nar/11.12.3889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  122. Stuart K. Kinetoplast DNA, mitochondrial DNA with a difference. Mol Biochem Parasitol. 1983 Oct;9(2):93–104. doi: 10.1016/0166-6851(83)90103-2. [DOI] [PubMed] [Google Scholar]
  123. Tait A. Evidence for diploidy and mating in trypanosomes. Nature. 1980 Oct 9;287(5782):536–538. doi: 10.1038/287536a0. [DOI] [PubMed] [Google Scholar]
  124. Turner M. J. Biochemistry of the variant surface glycoproteins of salivarian trypanosomes. Adv Parasitol. 1982;21:69–153. doi: 10.1016/s0065-308x(08)60275-4. [DOI] [PubMed] [Google Scholar]
  125. Van der Ploeg L. H., Bernards A., Rijsewijk F. A., Borst P. Characterization of the DNA duplication-transposition that controls the expression of two genes for variant surface glycoproteins in Trypanosoma brucei. Nucleic Acids Res. 1982 Jan 22;10(2):593–609. doi: 10.1093/nar/10.2.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  126. Van der Ploeg L. H., Cornelissen A. W., Michels P. A., Borst P. Chromosome rearrangements in Trypanosoma brucei. Cell. 1984 Nov;39(1):213–221. doi: 10.1016/0092-8674(84)90207-1. [DOI] [PubMed] [Google Scholar]
  127. Van der Ploeg L. H., Liu A. Y., Borst P. Structure of the growing telomeres of Trypanosomes. Cell. 1984 Feb;36(2):459–468. doi: 10.1016/0092-8674(84)90239-3. [DOI] [PubMed] [Google Scholar]
  128. Van der Ploeg L. H., Liu A. Y., Michels P. A., De Lange T., Borst P., Majumder H. K., Weber H., Veeneman G. H., Van Boom J. RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes. Nucleic Acids Res. 1982 Jun 25;10(12):3591–3604. doi: 10.1093/nar/10.12.3591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  129. Van der Ploeg L. H., Schwartz D. C., Cantor C. R., Borst P. Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules. Cell. 1984 May;37(1):77–84. doi: 10.1016/0092-8674(84)90302-7. [DOI] [PubMed] [Google Scholar]
  130. Van der Ploeg L. H., Valerio D., De Lange T., Bernards A., Borst P., Grosveld F. G. An analysis of cosmid clones of nuclear DNA from Trypanosoma brucei shows that the genes for variant surface glycoproteins are clustered in the genome. Nucleic Acids Res. 1982 Oct 11;10(19):5905–5923. doi: 10.1093/nar/10.19.5905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  131. Vervoort T., Barbet A. F., Musoke A. J., Magnus E., Mpimbaza G., Van Meirvenne N. Isotypic surface glycoproteins of trypanosomes. Immunology. 1981 Oct;44(2):223–232. [PMC free article] [PubMed] [Google Scholar]
  132. Walsh J. A., Warren K. S. Selective primary health care: an interim strategy for disease control in developing countries. N Engl J Med. 1979 Nov 1;301(18):967–974. doi: 10.1056/NEJM197911013011804. [DOI] [PubMed] [Google Scholar]
  133. Williams R. O., Young J. R., Majiwa P. A., Doyle J. J., Shapiro S. Z. Analyses of variable antigen gene rearrangements in Trypanosoma brucei. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1037–1042. doi: 10.4269/ajtmh.1980.29.1037. [DOI] [PubMed] [Google Scholar]
  134. Williams R. O., Young J. R., Majiwa P. A., Doyle J. J., Shapiro S. Z. Contextural genomic rearrangements of variable-antigen genes in Trypanosoma brucei. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):945–949. doi: 10.1101/sqb.1981.045.01.111. [DOI] [PubMed] [Google Scholar]
  135. Williams R. O., Young J. R., Majiwa P. A. Genomic environment of T. brucei VSG genes: presence of a minichromosome. Nature. 1982 Sep 30;299(5882):417–421. doi: 10.1038/299417a0. [DOI] [PubMed] [Google Scholar]
  136. Williams R. O., Young J. R., Majiwa P. A. Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei. Nature. 1979 Dec 20;282(5741):847–849. doi: 10.1038/282847a0. [DOI] [PubMed] [Google Scholar]
  137. Young J. R., Donelson J. E., Majiwa P. A., Shapiro S. Z., Williams R. O. analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei. Nucleic Acids Res. 1982 Feb 11;10(3):803–819. doi: 10.1093/nar/10.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  138. Young J. R., Miller E. N., Williams R. O., Turner M. J. Are there two classes of VSG gene in Trypanosoma brucei? Nature. 1983 Nov 10;306(5939):196–198. doi: 10.1038/306196a0. [DOI] [PubMed] [Google Scholar]
  139. Young J. R., Shah J. S., Matthyssens G., Williams R. O. Relationship between multiple copies of a T. brucei variable surface glycoprotein gene whose expression is not controlled by duplication. Cell. 1983 Apr;32(4):1149–1159. doi: 10.1016/0092-8674(83)90298-2. [DOI] [PubMed] [Google Scholar]
  140. Young J. R., Turner M. J., Williams R. O. The role of duplication in the expression of a variable surface glycoprotein gene of Trypanosoma brucei. J Cell Biochem. 1984;24(3):287–295. doi: 10.1002/jcb.240240309. [DOI] [PubMed] [Google Scholar]

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