Skip to main content
. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Biochemistry. 2013 May 21;52(22):3921–3929. doi: 10.1021/bi400295x

Figure 5.

Figure 5

Michaelis-Menten analysis of the FXase complex. FVIII (1 nM) was activated by 20 nM thrombin for 1 min, immediately reacted with hirudin (10 Unit/ml) and 40 nM FIXa, and FXa generation was initiated by adding the indicated concentrations of FX. The rate of generated FXa was measured as described in Methods. Data were fitted to Michaelis-Menten equation by non-linear least squares regression and fitted-lines were drawn. Each point represents the value averaged from three separate determinations. Symbols denote for WT (open circles) and Ser2029Cys/Pro2292Cys (open triangles).