Figure 1.

A) Gel filtration profile of cardiolipin synthetase (cls) isolated from Mycobacterium phlei; B) Thin layer chromatogram of cardiolipin synthesized by the cls isolated from Mycobacterium phlei. S-TLC: Phospholipids, Phosphatidyl ethanolamine (PE), Phosphatidyl glycerol (PG) and cardiolipin (CL) obtained from sigma, mp-TLC: Phospholipids, PG and CL obtained from the synthesis of CL by cls of M. phlei.; C) HPLC profile of cls obtained from Sephacryl S-200 column. 2.5 mg of cls purified on Sephacryl S-200 column was applied on HPLC-Gel permeation column [TSK G-3000SW (21.5 × 300 mm)]; D) RP-HPLC profile of cls obtained from HPLC-GPC column. 1 mg of cls purified from HPLC-GPC column was applied on C-18 RP-HPLC (Waters); E) 10% SDS-PAGE analysis of cls purified on HPLC. Lane 1. 5 µg of cls purified on Sephacryl S-200, lanes 2 and 3 5 µg of cls fractionated on HPLC-GPC lane 4 cls purified on C 18 RP HPLC, lane M. molecular size markers obtained from Bangalore genei pvt ltd.; F) Isoelectric focussing of gel pattern of purified cls; G) Effects of Mg²⁺, Mn²⁺ and Ca²⁺ on cls activity by using CDP-diacyl glycerol and phosphotidyl glycerol as substrates.