FIG 4 .

Delayed autolysis at 37°C of single mutants pcsB^L78S-L219P(Ts) and ftsX^V266A(Sup) and double pcsB^L78S-L219P(Ts) ftsX^S264L(Sup) and pcsB^L78S-L219P(Ts) ftsX^V266A(Sup) mutants. (A) Strains IU1945 (parent), IU4261 [pcsB^L78S-L219P(Ts)], IU6426 [ftsX^S264L(Sup)], IU6371 [pcsB^L78S-L219P(Ts) ftsX^S264L(Sup)], IU6439 [ftsX^V266A(Sup)], and IU6363 [pcsB^L78S-L219P(Ts) ftsX^V266A(Sup)] were grown in BHI broth at 32°C and diluted to an OD₆₂₀ of ~0.001 in fresh BHI broth prewarmed to the temperatures indicated. Growth was monitored as described in Materials and Methods, and representative growth curves from more than 3 independent experiments are shown. ftsX^S264L(Sup) and ftsX^V266A(Sup) are non-allele-specific and allele-specific suppressors of pcsB^CC(Ts), respectively (Fig. 1; see the text). A comparable delay in autolysis was detected for strain IU4262 [pcsB^A160P(Ts)] (see Fig. S3 in the supplemental material). (B) At an OD₆₂₀ of 0.7 in 37°C cultures, cells of strain IU1945 (parent), IU4261 [pcsB^L78S-L219P(Ts)], and IU4262 [pcsB^A160P(Ts)] were stained for viability as described in Materials and Methods. Experiments were performed independently three times (n = 3), and ~1,300 cells were counted per sample (B).