Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1985 Mar;49(3):627–633. doi: 10.1128/aem.49.3.627-633.1985

Transfer of Sucrose-Fermenting Ability and Nisin Production Phenotype among Lactic Streptococci

Carlos F Gonzalez 1,†,*, Blair S Kunka 1
PMCID: PMC373560  PMID: 16346756

Abstract

Transfer of sucrose fermentation ability, nisin production, and nisin resistance from Streptococcus lactis to S. lactis and Streptococcus lactis subsp. diacetylactis occurred between cells immobilized on nitrocellulose filters in the presence of DNase. Transconjugants were able to act as donors to transfer the Suc-Nis phenotype in subsequent mating. No changes in sensitivity to lytic phage c2 were noted in S. lactis transconjugants. However, temperature-independent restriction of lytic phage 18-16 was noted in transconjugants of S. lactis subsp. diacetylactis 18-16. Adsorption studies with phage-resistant transconjugants showed that resistance was not due to lack of adsorption by the lytic phage. Physical evidence for the presence of introduced plasmid DNA was not found in lysates of transconjugants.

Full text

PDF
627

Images in this article

628Image
on p.628
630Image
on p.630

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Efstathiou J. D., McKay L. L. Plasmids in Streptococcus lactis: evidence that lactose metabolism and proteinase activity are plasmid linked. Appl Environ Microbiol. 1976 Jul;32(1):38–44. doi: 10.1128/aem.32.1.38-44.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fuchs P. G., Zajdel J., Dobrzański W. T. Possible plasmid nature of the determinant for production of the antibiotic nisin in some strains of Streptococcus lactis. J Gen Microbiol. 1975 May;88(1):189–192. doi: 10.1099/00221287-88-1-189. [DOI] [PubMed] [Google Scholar]
  4. Gonzalez C. F., Kunka B. S. Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl Environ Microbiol. 1983 Jul;46(1):81–89. doi: 10.1128/aem.46.1.81-89.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kempler G. M., McKay L. L. Characterization of Plasmid Deoxyribonucleic Acid in Streptococcus lactis subsp. diacetylactis: Evidence for Plasmid-Linked Citrate Utilization. Appl Environ Microbiol. 1979 Feb;37(2):316–323. doi: 10.1128/aem.37.2.316-323.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kempler G. M., McKay L. L. Improved Medium for Detection of Citrate-Fermenting Streptococcus lactis subsp. diacetylactis. Appl Environ Microbiol. 1980 Apr;39(4):926–927. doi: 10.1128/aem.39.4.926-927.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kozar W., Rajchert-Trzpil M., Dobrzański W. T. The effect of proflavin, ethidium bromide and an elevated temperature on the appearance of nisin-negative clones in nisin-producing strains of Streptococcus lactis. J Gen Microbiol. 1974 Aug;83(2):295–302. doi: 10.1099/00221287-83-2-295. [DOI] [PubMed] [Google Scholar]
  8. LeBlanc D. J., Crow V. L., Lee L. N., Garon C. F. Influence of the lactose plasmid on the metabolism of galactose by Streptococcus lactis. J Bacteriol. 1979 Feb;137(2):878–884. doi: 10.1128/jb.137.2.878-884.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leblanc D. J., Lee L. N. Rapid screening procedure for detection of plasmids in streptococci. J Bacteriol. 1979 Dec;140(3):1112–1115. doi: 10.1128/jb.140.3.1112-1115.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Baldwin K. A. Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophage. Appl Environ Microbiol. 1984 Jan;47(1):68–74. doi: 10.1128/aem.47.1.68-74.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Neve H., Geis A., Teuber M. Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci. J Bacteriol. 1984 Mar;157(3):833–838. doi: 10.1128/jb.157.3.833-838.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Snook R. J., McKay L. L. Conjugal Transfer of Lactose-Fermenting Ability Among Streptococcus cremoris and Streptococcus lactis Strains. Appl Environ Microbiol. 1981 Nov;42(5):904–911. doi: 10.1128/aem.42.5.904-911.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES