(A) Schematic of the retinal circuitry with the cells recorded to obtain data presented in this figure highlighted. Labeling as in Figure 1. (B) Representative 2-photon image stack projected along two orthogonal axes. For visual clarity, the recording electrodes have been digitally removed from the side view (bottom panel). (C, D) Simultaneous voltage (I = 0 pA, VRest ~ −63 mV) and EPSC (VM ~ −60 mV) recording of an ON CBC and ON RGC, respectively, during stage III waves, shown on different timescales. (E) Crosscorrelation (mean ± SEM, n = 18) of ON CBC voltage (v) and excitatory synaptic conductance of ON RGCs (or inhibitory conductance of OFF RGC) (g). (F) Simultaneous voltage and EPSC recording of an OFF CBC (VRest ~ −46 mV) and ON RGC. (G) Crosscorrelation (n = 10) of OFF CBC voltage (v) and excitatory conductance of ON RGCs (or inhibitory conductance of OFF RGC) (g). (H) The average of the maximal voltage changes during each wave of a given CBC is indicated by circles (open - ON, filled - OFF). Population averages are shown by lines. (I) The conditional probability of algorithmically detecting a wave in an ON RGC EPSC trace given that a wave was identified in the simultaneously recorded CBC voltage is depicted. Thin lines indicate data from each dual recording. The thick line and filled symbols (errorbars) represent the mean (± SEM) of the population. See also Figure S2.