Table 1.

Development of first generation of Apoe^−/− mice by ICSI.

	Fresh spermatozoon group	ED spermatozoon group
No. of oocytes injected	380	364
No. of oocytes survived ICSI	149	128
ICSI survival rate (%)	39.2	35.2
No. of two-cell embryos obtained	137	114
Fertilization rate (%)	91.9	89.1
No. of pups born	52	20
Birth rate (%)	38.0	17.5*
Sex ratio (M/F)	25/27	12/8

ED, evaporatively dried. All ICSI-derived two-cell embryos were transferred for production of pups; ICSI survival rate (%), number of oocytes survived ICSI/number of injected oocytes; fertilization rate (%), number of two-cell embryos/number of oocytes survived ICSI; birth rate (%), number of pups born/number of two-cell embryos transferred.

^*Significantly different from the value in the same row (P<0.001).