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. Author manuscript; available in PMC: 2014 Feb 8.
Published in final edited form as: Nature. 2013 Jul 10;500(7461):217–221. doi: 10.1038/nature12298

Figure 1. Non-neural and pre-placodal ectoderm induction in 3D culture.

Figure 1

a, Non-neural ectoderm induction strategy. de, definitive ectoderm; me, mesendoderm; nne, non-neural ectoderm; ne, neuroectoderm. b-d, Morphology of control (Ctrl), BMP, and BMP/SB aggregates. e, SB decreases the level of brachyury expression induced by BMP (n=3; **P<0.01; mean ± s.e.m.). f-h, Brachyury+ cells are less prevalent in BMP/SB aggregates. i-k, BMP/SB aggregates contain an outer AP2/Ecad+ epithelium and an interior Sox1+ and Ncad+ cell layer. l, BMP/SB aggregate composition on day 5. m, Pre-placodal ectoderm induction strategy. epi, epidermis; ppe, pre-placodal ectoderm. n-p, BMP/SB-FGF/LDN are distinguished by a thickened AP2+ epithelium absent in other conditions. Scale bars, 100 μm.