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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Neuroscience. 2013 Apr 18;245:1–11. doi: 10.1016/j.neuroscience.2013.04.003

Table 1.

Timeline of experiments.

Days Experiment
Preday -30 Rats were purchased from Harlen Industries
Preday -27 Initial filament testing was performed
Preday -26 Cannulation of the trigeminal ganglia was completed
Preday -20 Cannulated rats were placed in the feeder units
Preday -14 Filament testing was performed
Preday -7 Filament testing was performed
Preday -3 Feeding data and body weight was analyzed
Preday -2 Feeding data and body weight was analyzed
Preday -1 Feeding data and body weight was analyzed
Lig +1 Ligature placed on the tendon on the morning of day ligature 1 and feeding data and body weight was analyzed
Lig +2 Feeding data and body weight was analyzed
Lig +3 Feeding data and body weight was analyzed and filament testing was performed
Lig +4/siRNA +1 siRNA infused into the trigeminal ganglia on the morning of day siRNA 1 and feeding data and body weight was analyzed
Lig +5/siRNA +2 Feeding data and body weight was analyzed
Lig +6/siRNA +3 Feeding data and body weight was analyzed and filament testing was performed
Sacrifice 72 h after siRNA infusion the animals were sacrificed and the trigeminal ganglia and trigeminal nucleus caudalis tissue was isolated