Fig. 1.
Identification of Su(Hw) target genes. (A) Shown are 4- to 6-hour-old DAPI-stained ovarioles isolated from fertile [su(Hw)+/+] and sterile [su(Hw)2/v and su(Hw)Pb/2] females. Scale bars: 25 μm. (B) Microarray analyses identified 175 increased (red bar) and 122 decreased (blue bar) genes, of which 75 and 30 correspond to Su(Hw) target genes, respectively (gray bars). (C) Quantitative PCR validation of target genes. Expression is normalized to the housekeeping gene RpL32 and shown as heat map of fold change values relative to su(Hw)+/+, with blue and red indicating lower and higher expression, respectively. 33/93†, heteroallelic combination of two independently generated recombinant chromosomes containing double Cp190H4-1 and mod(mdg4)u1 mutations. The gypsy insulator proteins associated with the SBSs are shown. Three non-target housekeeping genes and su(Hw) were included as controls. Two independent biological samples (1, 2) were analyzed.