Table 2.
Toxicity to hematopoietic progenitors cultured in MethoCult for 14 days in the presence of HXR9 alone and in combination with ch128.1Av.
| Colony Type | Donor 1 | Donor 2 | Donor 3 | |
|---|---|---|---|---|
| Control (Buffer alone) | ||||
| CFU-E | 31 ± 3.3 | 12 ± 4.9 | 19 ± 3.6 | |
| BFU-E | 40 ± 5.7 | 16 ± 6.4 | 28 ± 5.0 | |
| CFU-GM | 54 ± 5.2 | 29 ± 0.6 | 38 ± 3.3 | |
| CFU-GEMM | 5 ± 0.8 | 3 ± 0.6 | 6 ± 3.4 | |
| 50 µM HXR9 | ||||
| CFU-E | 4 ± 3.0** | 4 ± 2.2* | 2 ± 1.9** | |
| BFU-E | 5 ± 2.6** | 5 ± 2.8* | 3 ± 1.6** | |
| CFU-GM | 6 ± 1.7** | 8 ± 1.6** | 6 ± 2.6** | |
| CFU-GEMM | 0 ± 0.0* | 1 ± 1.0* | 1 ± 0.8* | |
| 20 µM HXR9 | ||||
| CFU-E | 14 ± 2.2** | 10 ± 2.2 | 5 ± 1.9** | |
| BFU-E | 24 ± 5.4* | 8 ± 1.9 | 8 ± 1.5** | |
| CFU-GM | 52 ± 5.5 | 21 ± 2.2* | 21 ± 5.3** | |
| CFU-GEMM | 3 ± 1.4 | 1 ± 0.6* | 1 ± 0.8* | |
| 10 µM HXR9 | ||||
| CFU-E | 17 ± 2.2** | 9 ± 2.2 | 9 ± 3.8* | |
| BFU-E | 30 ± 6.7 | 12 ± 4.7 | 16 ± 3.4* | |
| CFU-GM | 60 ± 9.0 | 27 ± 2.1 | 21 ± 5.8* | |
| CFU-GEMM | 4 ± 3.4 | 3 ± 1.3 | 4 ± 3.5* | |
| 2.5 nM ch128.1Av | ||||
| CFU-E | 13 ± 9.8* | 4 ± 0.8* | 8 ± 3.7* | |
| BFU-E | 26 ± 7.4* | 3 ± 1.4* | 7 ± 1.8** | |
| CFU-GM | 26 ± 4.5** | 14 ± 3.9* | 18 ± 2.8** | |
| CFU-GEMM | 1 ± 1.4* | 0 ± 0.0* | 0 ± 0.0* | |
| Combination (20 µM HXR9 + 2.5 nM ch128.1Av) |
||||
| CFU-E | 3 ± 1.0** | 4 ± 2.2* | 4 ± 3.6* | |
| BFU-E | 3 ± 1.6** | 2 ± 2.7* | 4 ± 2.7** | |
| CFU-GM | 5 ± 2.3** | 8 ± 2.2** | 11 ± 0.6** | |
| CFU-GEMM | 0 ± 0.0* | 0 ± 0.5* | 1 ± 0.5* | |
CFU-E: colony forming units-erythroid; BFU-E: burst forming units-erythroid; CFU-GM: colony forming units-granulocyte/machrophage; CFU-GEMM: colony forming units-granulocyte/erythrocyte/macrophage/megakaryocyte. Data represents the mean of quadruplicates ± the standard deviation
**
p < 0.001,
*
p < 0.05 compared with control cells treated with diluent alone.