Natural AChE Inhibitors from Plants and their Contribution to Alzheimer’s Disease Therapy

Abstract

As acetylcholinesterase (AChE) inhibitors are an important therapeutic strategy in Alzheimer’s disease, efforts are being made in search of new molecules with anti-AChE activity. The fact that naturally-occurring compounds from plants are considered to be a potential source of new inhibitors has led to the discovery of an important number of secondary metabolites and plant extracts with the ability of inhibiting the enzyme AChE, which, according to the cholinergic hypothesis, increases the levels of the neurotransmitter acetylcholine in the brain, thus improving cholinergic functions in patients with Alzheimer’s disease and alleviating the symptoms of this neurological disorder. This review summarizes a total of 128 studies which correspond to the most relevant research work published during 2006-2012 (1st semester) on plant-derived compounds, plant extracts and essential oils found to elicit AChE inhibition.

INTRODUCTION

Alzheimer’s disease (AD) is a progressive neuro-degenerative disorder associated with memory impairment and cognitive deficit. It is characterized by low levels of acetylcholine in the brain of AD patients. According to the cholinergic hypothesis, the inhibition of acetylcholinesterase (AChE), an enzyme that catalyzes acetylcholine hydrolysis, increases the levels of acetylcholine in the brain, thus improving cholinergic functions in AD patients. Further-more, although the general consensus concludes that AChE inhibitors (AChEi) can alleviate AD symptoms, they neither delay nor reverse the disease progress. Most of the drugs currently available for the treatment of AD are AChEi: tacrine (1), donezepil (2), rivastigmine (3) and galanthamine (4), all of which have limited effectiveness and some kind of side effect [1]. Tacrine (1) and donepezil (2), both from synthetic origin, were the first drugs approved for the treatment of cognitive loss in AD patients by US-FDA in 1993 and 1996, respectively. Rivastigmine (3) was approved in 2000 (US-FDA) and was designed from the lead compound physostigmine, a natural AChEi alkaloid. Galanthamine (4), a natural alkaloid first obtained from Galanthus spp. was approved by US-FDA in 2001. Huperzine A (5), an alkaloid found in Huperzia spp., is an AChEi commercialized as a dietary supplement for memory support and it is used to treat AD symptoms in China. This alkaloid has been thoroughly studied with promising results yielded particularly from the evaluation of cognitive performance of animals as well as from studies on its efficacy, tolerance and safety.

Taking into account that inhibitors 3, 4 and 5 are related to natural products and that AChEi are an important therapeutic strategy for the treatment of AD, many research groups have focused their studies on naturally-occurring compounds from plants as potential sources of either new or more effective AChEi. These studies led to the discovery of an important number of secondary metabolites as well as plant extracts, both of which are characterized by their ability to inhibit AChE. On the other hand, the fact that a significantly relevant number of research papers has been recorded in this field during the last decades can be clearly attributed to the development of colorimetric methods which allow a rapid and facile screening of a large number of samples. Ellman’s method is the most widely used for the detection of AChEi, even in complex mixtures, and for the quantification of anti-AChE inhibitory activity [2-6].

Several reviews on the newly discovered AChEi obtained from plants, fungus and marine organisms have also been published over the last years [7-10]. The majority of these AChEi belong to the alkaloid group, including indole, isoquinoline, quinolizidine, piperidine and steroidal alkaloids. On the other hand, several non-alkaloidal and potent AChEi have been obtained from natural sources, including terpenoids, flavonoids and other phenolic compounds. Interestingly, although literature demonstrates to be rich in the study on AChEi obtained from plants, this issue keeps on being the center of attention for research as confirmed by the increasing number of studies published every year. Therefore, the purpose of this review is to provide a comprehensive summary of the literature, particularly that published during 2006-2012 (1^st semester) on plant-derived compounds, plant extracts and essential oils which have been reported to inhibit AChE. Readers interested not only in previous findings but also in synthetic/semisynthetic AChEi or natural AChEi of fungal, marine or microbial origin are recommended to see the above-mentioned reviews i.e. [7-10]. For the sake of brevity and in order to focus our attention on the most relevant findings, only those research papers reporting quantified results (IC₅₀ and/or percentage of inhibition at a given concentration) were included. Extracts or essential oils with IC₅₀ > 0.5 mg/ml were considered weakly active and were therefore not taken into account in the present review. With a few exceptions, only molecules with IC₅₀ < 50 µM have been considered. Furthermore, unless otherwise stated, those results on AChE inhibition included in the present review refer to in vitro assays carried out with AChE from electric eel.

ALKALOIDS WITH AChE INHIBITORY ACTIVITY

The quinoline alkaloids 3-hydroxy-2,2,6-trimethyl-3,4,5,6-tetrahydro-2H-pyrano[3,2-c] quinoline-5-one (6), ribalinine (7) and methyl isoplatydesmine (8) isolated from the aerial parts of Skimmia laureola (Rutaceae) were found to be linear mixed inhibitors of AChE with K_i = 110.0, 30.0 and 30.0 µM, respectively [11]. These alkaloids were also observed to evidence butyrylcholinesterase (BChE) inhibition.

On the other hand, of the several alkaloids that were isolated from the active extracts of Esenbeckia leiocarpa (Rutaceae), leptomerine (9) and kokusaginine (10) with IC₅₀ values of 2.5 and 46 µM, respectively, were observed to elicit AChE inhibitory activity [12]. The isolation of skimmianine (11), a furoquinoline alkaloid with very low AChE inhibitory activity, was also reported by the same authors. This alkaloid was observed in another Rutaceae, Zanthoxylum nitidum, exhibiting a moderate AChE inhibitory activity (IC₅₀ = 8.6 µg/ml) [13].

Nelumbo nucifera is a well-known medicinal plant belonging to the Nelumbonaceae family which was studied due to its therapeutic potential [14]. N-methylasimilobine (12), an aporphine alkaloid with an IC₅₀ = 1.5 µg/ml which was found to be a non-competitive inhibitor, was recently isolated from this plant [15]. In a random screening, two extracts of Beilschmiedia species were observed to exhibit AChE inhibition and a phytochemical study of B. alloiophylla and B. kunstleri revealed the presence of several alkaloids with IC₅₀ values ranging between 2.0 and 10.0 µM [16]. The most potent AChEi were found to be 2-hydroxy-9-methoxyaporphine (13), laurotetanine (14), liriodenine (15) and oreobeiline (16) (IC₅₀ = 2.0-5.0 µM), with anti-AChE activity comparable to huperzine A (IC₅₀ = 1.8 µM). A significant AChE inhibitory activity was also observed in secoboldine (17), boldine (18), isoboldine (19), asimilobine (20) and 3-methoxynordomesticine (21) (IC₅₀ = 8.4 - 10.0 µM).

Research on plants from the genus Corydalis (Papaveraceae) which are used for the treatment of memory dysfunction in folk medicine reported the presence of benzylisoquinoline alkaloids with anti-AChE activity [7]. The ethanolic extract obtained from the tuber of C. turtschaninovii previously found to elicit AChE inhibition was selected to carry out a chemical study which led to the isolation of the isoquinoline alkaloids stylopine (22), epiberberine (23), pseudodehydrocorydaline (24), pseudocopsitine (25) and pseudoberberine (26). In the assay with mouse brain cortex as a source of AChE enzyme, the IC₅₀ values obtained for each of these alkaloids were 15.8, 6.5, 8.4, 4.3 and 4.5 µM, respectively [17]. In addition, alkaloids 25 and 26, the two most active compounds, were found to elicit anti-amnesic activity [17, 18]. Alkaloids with benzylisoquinoline skeleton from Corydalis species having aromatic methylenedioxy groups and a quaternary atom of nitrogen were observed to show the strongest AChE inhibition [7, 17, 18]. In a more recent work, six protoberberine alkaloids 23, 27 - 31, were identified in rhizomes of Coptis chinensis which are traditionally used in Chinese medicine for the treatment of various diseases. Coptidis rhizomes and their alkaloids were reported to have cognitive-enhancing and neuroprotective effects and the analysis of the anti-AChE activity of these alkaloids showed that the IC₅₀ values of berberine (27), palmatine (28), jateorrhizine (29), coptisine (30) and groenlandicine (31) ranged between 0.44 and 0.80 µM while that of epiberberine (23) was slightly higher (IC₅₀ = 1.07 µM) [19]. Of these alkaloids, compounds 27, 30 and 31 were observed to have an aromatic methylenedioxy group. In this study groenlandicine (31) and berberine (27) were found to be the most active as BChE inhibitors and epiberberine (23) was observed to significantly inhibit β-secretase (BACE1) [19].

The alkaloids (+)-canadaline (32) and (+)-canadine (33), both isolated from Corydalis cava and with an IC₅₀ = 20.1 and 12.4 µM, respectively, were observed to elicit a moderate inhibitory activity when tested with AChE from human blood [20].

On the other hand, Stephania venosa (Menispermaceae), a Thai medicinal plant, was found to show a high AChE inhibitory activity. The ethanolic extract of S. venosa was subjected to bioassay-guided fractionation to identify AChEi [21]. The following moderately active quaternary protoberberine alkaloids could be isolated: stepharanine (34), cyclanoline (35) and N-methyl stepholidine (36) with IC₅₀ values of 14.10, 9.23 and 31.30 µM, respectively. A similar fractionation approach was followed to identify the compounds responsible for AChE inhibition in Chelidonium majus (Papaveraceae) [22]. Three active constituents were identified, namely 8-hydroxydihydrochelerythrine (37), 8-hydroxydihydrosanguinarine (38) and berberine (27). Compounds 37 and 38, with no previous record as AChEi, were found to elicit significant anti-AChE activity with an IC₅₀ = 0.61 and 1.37 µM, respectively.

Taspine (39) was isolated from the alkaloid-enriched extract obtained from Magnolia x soulangiana ^{(Magnoliaceae) [23]}. This alkaloid was found not only to show a dose-dependent and long-lasting inhibitory effect on AChE (IC₅₀ = 0.33 µM) but also to be more potent than galanthamine (IC₅₀ = 3.2 µM) although its inhibitory activity is comparable to that of tacrine (IC₅₀ = 0.22 µM). Similar observations were obtained when the in vitro assay was performed with human AChE (IC₅₀ = 0.54 µM). Compound 39 resulted to be inactive against BChE, acting as a selective AChEi.

Catharanthus roseus (Apocynaceae) is a plant mainly known as a source of vincristine and vinblastine, two alkaloids found in its leaves and appreciated as anticancer compounds. Several other compounds with biological importance can be also found in C. roseus. For example, the alkaloid serpentine (40), isolated from the roots of this plant, was reported to be a potent in vitro AChEi (IC₅₀ = 0.775 µM) compared with physostigmine (IC₅₀ = 6.45 µM) [24].

A bioassay-guided fractionation from the stems of Ervatamia hainanensis (Apocynaceae), a plant used in traditional Chinese medicine, allowed the isolation of several monoterpenoid indole alkaloids, some of them showing a potent AChE inhibitory activity [25]. For example, coronaridine (41) and voacangine (42), differing from each other only by the methoxy group attached to the aromatic ring, were observed to have an IC₅₀ = 8.6 and 4.4 µM, respectively, these values being similar to that of galanthamine (3.2 µM). On the other hand, 10-hydro-xycoronaridine (43) was found to evidence a reduced AChE inhibition (IC₅₀ = 29 µM), which was attributed to the introduction of a hydroxyl group to the aromatic ring. The indole alkaloids coronaridine (41) and voacangine (42), both detected in the stalks of Tabernaemontana australis (Apocynaceae), had been formerly identified as AChEi but no inhibition values were reported [26].

The genus Tabernaemontana is known for the wide variety of unusual bioactive indole alkaloids it produces. Among them, the bisindole alkaloids isolated from T. divaricata roots are an interesting example of new structures with potent AChE inhibitory activity. The crude alkaloid extract obtained from the root of T. divaricata was found to yield four bisindole alkaloids 44 - 47 [27]. The analysis of AChE inhibition revealed that 19,20-dihydrotabernamine (44) and 19,20-dihydroervahanine A (45) strongly inhibit AChE, with an IC₅₀ = 0.227 and 0.071 µM, respectively, thus showing that they are significantly more active than galanthamine (IC₅₀ = 0.594 µM). The fact that inhibition was found to be higher for compound 45 than for compound 44 suggests that the introduction of a carbomethoxy group at C16’ increases the enzymatic inhibition. In addition, taking into account that conodurine (46) and tabernaelegantine (47) were found to show no activity in AChE, it was suggested that the substitution at C11’ and C12’ is relevant for AChE inhibitory activity [27].

Uncaria rhynchophylla (Rubiaceae) is a Chinese medicine herb used to treat epilepsy. The alkaloid fraction from U. rhynchophylla is known for its antiepileptic and neuroprotective effects. Geissoschizine methyl ether (48), a strong AChEi, as well as six other weakly active alkaloids were recently isolated from this herb [28]. The active compound 48 was observed to inhibit AChE in a reversible and non-competitive way with an IC₅₀ = 3.7 µg/ml.

The study of AChE inhibitory activity of Brazilian apocynacea Himatanthus lancifolius, commonly known as “agoniada”, led to the identification of active extracts in this plant and allowed the isolation of uleine (49), an active indole alkaloid, at a high concentration in the alkaloid fraction. The IC₅₀ value observed for this alkaloid was 0.45 µM [29].

As to the Amaryllidaceae family, phytochemical research conducted in the last decades on this family revealed several alkaloids with moderate or potent inhibition of AChE [3, 7, 30]. In the search of new natural sources of galanthamine and other Amaryllidaceae alkaloids with anti-AChE activity, bulbs and leaves of Hippeastrum papilio collected in the South of Brazil were studied. Galanthamine (4), the already known alkaloids narwedine (50), haemanthamine (51), 11-hydroxyvittatine (52), 8-O-demethylmaritidine (53) and vittatine (54) as well as the new alkaloid 11(-hydro-xygalanthamine (55) were all isolated and of all of them galanthamine was obtained in significant amounts [31]. Compound 55 was observed to elicit AChE inhibition as other galanthamine-type alkaloids do, with an IC₅₀ = 14.5 µM. Furthermore, because habranthine, epimer of 55, was observed to have an anti-AChE activity similar to that of galanthamine, it was concluded that β configuration at C11 is unfavorable for the interaction with AChE [3, 31]. Other potent AChEi, such as N-allylnorgalanthamine (56) and N-(14-methylallyl)norgalanthamine (57), were isolated from Leucojum aestivum, an amaryllidacea used for the industrial extraction of galanthamine [32]. N-alkylated galanthamine derivatives 56 and 57 were isolated together with galanthamine (4), epinorgalanthamine (58), narwedine (50) and lycorine (59), from the mother liquors obtained after the industrial production of galanthamine. Alkaloids 56 and 57, with IC₅₀ values of 0.18 and 0.16 µM, respectively, resulted to be ten times more potent AChEi than galanthamine (IC₅₀ = 1.82 µM).

The chemical investigation of Galanthus rizehensis, a wild-growing species from Turkey, allowed the isolation of two new Amaryllidaceae alkaloid N-oxides, incartine N-oxide (60) and lycorine N-oxide (61) and seven known alkaloids namely, 1-acetyl-β-carboline (62), incartine (63), N-trans feruloyltyramine (64), lycorine (59), O-methylnorbelladine (65), vittatine (54) and 11-hydroxyvittatine (52) [33]. The potential of these alkaloids as AChEi was analyzed but only incartine N-oxide (60) was observed to elicit a moderate inhibitory activity (IC₅₀ = 34.50 µM), incartine (63) was observed to be weakly active (IC₅₀ = 106.97 µM) and the other alkaloids were found to be inactive. In a bioassay-guided fractionation of an active extract obtained from bulbs of Nerine bowdenii, the Amaryllidaceae alkaloid undulatine (66) was identified as the most active component of the alkaloid fraction, with an IC₅₀ = 37 µM [34].

Although benzylphenethylamine alkaloids were considered to belong exclusively to the Amaryllidaceae, some of them have been found to belong to other families [35]. A new example of this exception was found through the chemical investigation of Hosta plantaginea (Liliaceae) [36]. Seventeen benzylphenetylamine alkaloids, including five new alkaloids, 67-71, along with twelve known compounds [7-deoxy-trans-dihydronarciclasine, O-methyllycorenine, albomaculine, haemanthamine, O-demethylhaemanthamine, 8-O-demethylmaritadine, haemanthidine, yemenine C, lycorine, pseudolycorine, ungeremine (72) and norsanguinine (73)] were obtained. Some of these alkaloids were analyzed to determine whether they are AChEi or not.. Ungeremine (72) (IC₅₀ = 3.85 µM), norsanguinine (73) (IC₅₀ = 1.43 µM) and 8-demethoxy-10-O-methylhostasine (69) (IC₅₀ = 2.32 µM) were all found to be potent AChE inhibitors.

After the isolation of the potent AChEi huperzine A (5) from Huperzia serrata (Lycopodiaceae), several plants belonging to the genus Lycopodium have been investigated in an attempt to find alkaloids with unusual skeletons that could have AChE inhibitory activity [7, 8, 37].Five new Lycopodium alkaloids, 11(-hydroxyfawcettidine (74), 2(,11(-dihydroxyfawcettidine (75), 8(,11(-dihydro-xyfawcettidine (76), 2β-hydroxylycothunine (77) and 8(-hydroxylycothunine (78), with the fawcettimine skeleton were isolated from L. serratum, along with three known alkaloids, lycothunine (79), serratine (80) and serratanidine (81) [38]. AChE inhibitory activity was analyzed for the alkaloid lycoposerramine-H (82) previously isolated from L. serratum [39] and for compounds 74, 75, 78. Alkaloids 75 and 82 were observed to inhibit AChE with an IC₅₀ = 27.9 and 16.7 µM, respectively, while 74 and 78 were observed to show no anti-AChE activity. In another study, three new alkaloids (83 - 85) were isolated from L. carinatum, a species collected in Malasya [40]. Carinatumins A (83) and B (84) were observed to inhibit AChE from bovine erythrocytes with an IC₅₀ = 4.6 and 7.0 µM, respectively, whereas carinatumin C (85) was observed to show no inhibition (IC₅₀ > 100 µM). Alkaloids 83 and 84 were observed to exhibit an AChE inhibitory activity similar to that of huperzine A and huperzine B (IC₅₀ = 0.8 and 8.0 µM). Alkaloids from L. casuarinoides were also isolated and three new compounds, lycoparins A-C (86 - 88), were characterized, of which lycoparin C (88) was found to show a moderate AChE inhibitory activity (from bovine erythrocytes) with an IC₅₀ = 25 µM [41]. Lycoparin A (86) and lycoparin B (87), both having a carboxylic acid at C-15 and one or two N-methyl groups, were found to show no inhibitory activity.

As to Sarcococca and Buxus species (Buxaceae), they are known to produce steroidal alkaloids, some of which were observed to evidence strong AChE inhibition [7, 42, 43]. New steroidal alkaloid AChEi from S. saligna and S. hookeriana were recently found. In the case of S. saligna, the study –which was a continuation of previous research [44, 45]– of the bioactive steroidal alkaloids of this species allowed the isolation of five new compounds (89-93) and two already known bases (94 and 95) [46]. The new alkaloids 5,14-dehydro-N_a-demethylsaracodine (89), 14-dehydro-N_a-demethylsaracodine (90), 16-dehydrosarcorine (91), 2,3-dehydrosarsalignone (92) and 14,15-dehydro-sarcovagine D (93), as well as the known compounds sarcovagine C (94) and salignarine C (95) were analyzed as anti-AChE agents. Only 91, 92 and 95 were observed to exhibit significant AChE inhibition (IC₅₀ = 12.5, 7.0 and 19.7 µM, respectively). Compounds 89 - 92, 94 and 95 were also found to elicit strong and selective BChE inhibition [46]. The bioassay-guided chemical investigation of S. hookeriana allowed the isolation of two new pregnane-type steroidal alkaloids, hookerianamide H (96) and hookerianamide I (97) together with the known alkaloids N_a-methylepipachysamine D (98), sarcovagine C (94) and dictyophlebine (99) [47]. Compounds 94, 96, 97, 98 and 99 were tested for their inhibitory properties towards AChE and all of them were observed to elicit significant inhibitory activity (IC₅₀ 2.9 – 34.1 µM) as well as a potent anti-BChE activity (IC₅₀ 0.3 – 3.6 µM). Further studies on S. hookeriana yielded two new 5α-pregnane-type steroidal alkaloids, hookerianamides J (100) and K (101) [48]. Furthermore, eight known steroidal alkaloids, hookerianamide H (96) and hookerianamide I (97), chonemorphine (102), N-methylpachysamine A (103), epipachysamine-E-5-en-4-one (104), vagenine A (105), 2,3-dehydrosarsalignone (92) and sarcovagine C (94), were isolated and characterized. Alkaloids 94, 100, 101, 102, 103 and 104 were analyzed as AChEi. Compounds 100, 101, 102 and 103 were observed to inhibit AChE moderately (IC₅₀ 22.1 – 48.5 µM) while 104 and 94 were found to be more active inhibitors (IC₅₀ 9.9 and 8.1 µM, respectively).

Phytochemical research on Buxus hyrcana allowed the identification of several Buxus alkaloids with cholinesterase inhibitory activity [43, 49]. Three new triterpenoidal alkaloids, namely 17-oxo-3-benzoylbuxadine (106), buxhyrcamine (107) and 31-demethylcyclobuxoviridine (108) along with sixteen known compounds, all tested as AChEi, were isolated and characterized in a recent study on B. hyrcana collected from Iran [50]. Weak AChE inhibitory activity was observed for N_b-dimethylcyclobuxoviricine (109), papillozine C (110), cyclobuxophylline O (111) and arbora-1,9(11)-dien-3-one (112) (IC₅₀ = 35.4 - 47.9 µM). In the same in vitro assay, 17-oxo-3-benzoylbuxadine (106), buxhyrcamine (107), homomoenjodaramine (113), buxmicrophylline F (114), buxrugulosamine (115), moenjodaramine (116) and N₂₀-formyl-buxaminol E (117) were observed to show moderate AChE inhibition (IC₅₀ = 17.6 - 25.5 µM) while spirofornabuxine (118) was found to elicit a strong AChE inhibitory activity (IC₅₀ = 6.3 µM).

The crude methanolic extract of B. natalensis, a plant used to improve memory in the elderly by traditional healers in South Africa, was found to elicit AChE inhibition (IC₅₀ = 28 µg/mL).The phytochemical study of this extract yielded seven compounds 119 - 125 which were found to show either moderate or strong AChE inhibition [51]. The alkaloids O²-natafuranamine (119), O¹⁰-natafuranamine (120), cyclonataminol (121) and 31-demethylbuxaminol (122) were isolated and characterized for the first time while buxaminol A (123) was isolated for the first time as a natural product. Buxafuranamide (124) and buxalongifolamidine (125) were already known compounds. Compounds 119, 120 and 124 were observed to exhibit a significantly higher AChE inhibitory activity compared to the rest, with IC₅₀ values of 3.0, 8.5, and 14.0 µM, respectively. Compounds 121, 122, 123 and 125 were observed to be less effective as AChEi (IC₅₀ = 22.9 – 30.2 µM).

The bulbs of Fritillaria species (Lilliaceae) which are known to be a traditional medicinal herb called “Beimu” in China are used as an antitussive, antiasthmatic and expectorant agent. In the past, in a chemical study carried out on alkaloids from F. imperialis bulbs new steroidal alkaloids with weak AChE inhibition and great selectivity towards BChE were identified [52]. Thus, taking into account this previous study, the bulbs from five Fritillaria species were studied and their alkaloids were identified and evaluated as cholinesterase inhibitors. Eighteen alkaloids were isolated and their effects on human whole blood cholinesterase were assayed. Results showed that N-demethyl-puqietinone (126) from F. puqiensis, hupeheninoside (127) from F. hupehensis, ebeiedinone (128) from F. ebeiensis var. purpurea, yibeinoside A (129) from F. pallidiflora and chuanbeinone (130) from F. delavayi showed good AChE inhibition, with IC₅₀ values of 6.4, 16.9, 5.7, 6.5 and 7.7 µM, respectively. However, all of them were weaker AChEi than galanthamine (IC₅₀ = 1.9 µM). Compounds 127, 128, 129 and 130 were found to be stronger inhibitors on plasma BChE than galanthamine, the positive control [53].

In addition, the following steroidal alkaloids: conessine (131), isoconessimine (132), conessimin (133), conarrhimin (134) and conimin (135) were isolated in a bioassay-guided fractionation from the seeds of Holarrhena antidysenterica (Apocynaceae), a common Tibetan drug [54]. Compounds 131, 133, 134 and 135 were identified as active constituents against AChE. Conessimin (133) was found to be the strongest AChE inhibitor with an IC₅₀ = 4 µM whereas conessine (131), conarrhimin (134) and conimin (135) were found to be moderate AChE inhibitors (IC₅₀ = 21 - 28 µM). These findings indicate that the elimination of the N-methyl group of pyrrolidine moiety induces a significant increase of activity while the cleavage of either one or two N-methyl groups at C-3 position reduces the inhibitory potency. Compound 133 was selected for a kinetic study through which it was demonstrated that its AChE inhibitory activity is both reversible and non-competitive. Molecular docking simulations of these compounds with AChE helped to understand their interactions with AChE and were consistent with the experimental results obtained [54].

NON-ALKALOIDAL COMPOUNDS WITH AChE INHIBITORY ACTIVITY

In spite of the fact that the majority of the most potent inhibitors known to date are alkaloids, several non-alkaloidal AChEi from the plant kingdom and with different structural characteristics (terpenoids, sterols, flavonoids and phenolic compounds, etc) have been recognized as promising lead compounds as anti-AD agents [7-10]. Until 2006 only a few diterpenoids demonstrated to inhibit AChE [7]. However, further recent research has reported a larger number of compounds belonging to this group with the ability to exert either moderate or strong AChE inhibitory activity. In addition, a new cassane diterpene named niloticane (136) was isolated from the ethyl acetate bark extract of Acacia nilotica subsp. kraussiana (Fabaceae), a plant used in African traditional medicine [55]. Niloticane (136) was found to show an AChE inhibitory activity similar to that of the positive control galanthamine (IC₅₀ = 4 and 2 µM, respectively). In addition, one new (137) and six known (138 - 143) labdane-type diterpenoids were identified as AChE inhibitors present in an active extract obtained from Leonurus heterophyllus (Lamiaceae) by bioassay-guided fractionation [56]. Anti-AChE activity in 137 – 143 was analyzed in rat brain cortex as a source of AChE enzyme. Leoheteronin A (141) and leopersin G (143), both having a 15,16 epoxy group, were observed to be strong inhibitors with IC₅₀ values of 11.6 and 12.9 µM, respectively. The new compounds leoheteronin F (137) and leoheteronin D (142) were found to show moderate inhibition with IC₅₀ values of 16.1 and 18.4 µM, respectively. Leoheterin (138), hispanone (139) and galeopsin (140), all having a furan ring at the side chain, were found to be weakly active (IC₅₀ = 38.5 - 42.7 µM).

Asparagus adscendens (Asparagaceae) is a medicinal plant traditionally used as a nerve tonic and remedy for memory impairments in Pakistan. Conypododiol (144), which was isolated from the chloroform fraction of the methanolic extract of A. adscendens, was found to elicit AChE and BChE inhibition with an IC₅₀ = 2.17 and 11.21 µM, respectively [57]. This dual cholinesterase inhibitor was also observed to show potential as a bivalent ligand in molecular docking studies. Four non-competitive AChEi 145 – 148 were obtained in the chemical investigation of Ajuga bracteosa (Lamiaceae), another medicinal plant from Pakistan [58]. The diterpenoid dihydroajugapitin (148) was found to be the most active against AChE with an IC₅₀ = 14.0 µM. Compared to compound 148, lupulin A (147), clerodinin A (146) and dihydroclerodin (145) were observed to be less efficient inhibitors (IC₅₀ = 19.2, 26.5 and 35.2, respectively) and diterpenoids 145 - 148 were observed to elicit BChE inhibition. These findings indicate that the presence of a methoxy group at C-15 increases cholinesterase inhibitory potential.

From the methanolic extract of Haloxylon recurvum (Chenopodiaceae), a plant used in Pakistan for the treatment of several neuronal disorders, four new C-24 alkylated sterols 149 – 152 and five known sterols 153 – 157 were isolated [59]. Compounds 149 – 157 were analyzed as AChEi and were found to inhibit AChE in a concentration-dependent manner acting as non-competitive inhibitors. Haloxysterol B (150) and haloxysterol C (151), whose IC₅₀ values were 0.89 and 1.0 µM, respectively, were found to be the most active AChE inhibitors. Their inhibitory activity was observed to be similar to that of galanthamine (IC₅₀ 0.5 µM). Haloxysterol A (149) and 24-ethyl-cholest-6-ene-3,5-diol (157) were also observed to show potent AChE inhibition with IC₅₀ values of 8.3 and 3.5 µM, respectively. Haloxysterol D (152), 5(,8(-epidioxy-(24S)-ethyl-cholest-6,9(11),22(E)-triene-3β-ol (153), (24S)-ethyl-cholest-7,9(11),22(E)-triene-3β-ol (154), lawsaritol (155) and 24-ethyl-cholest-7-ene-3,5,6-triol (156) were found to elicit a moderate anti-AChE activity (IC₅₀ = 13.7 - 26.4 µM).

On the other hand, a bioassay-guided fractionation on the bark of Mesua elegans (Clusiaceae) allowed the isolation of the anti-AChE components responsible for the activity observed for the extract. Mesuagenin B (158) was the most potent inhibitor (IC₅₀ = 0.7 µM) and mesuagenin A (159), mesuagenin D (160) and 5,7-dihydroxy-8-(3-methylbutanoyl)-6- (E)-3,7-dimethylocta-2,6-dienyl-4-phenyl-2H-chromen-2-one (161) were observed to elicit strong AChE inhibition with IC₅₀ values of 1.06, 8.73 and 3.06 µM, respectively [60]. This bioassay-guided study is the first report of 4-phenylcoumarins as AChEi.

In the past, some examples of xanthones with moderate AChE inhibitory activity were reported [7]. Further recent research introduced two new xanthones, 162 and 163, to this group of AChEi also with moderate inhibitory activity. Macluraxanthone (162) which was obtained from the root of Maclura pomifera (Moraceae) was found to elicit non-competitive AChE inhibition (IC₅₀ = 8.47 µM) [61]. Furthermore, docking studies yielded results supporting in vitro results. Triptexanthoside C (163) which was isolated from the methanolic extract of Gentianella amarella ssp. acuta (Gentianaceae) was observed to elicit AChE inhibition with an IC₅₀ = 13.8 µM [62].

The methanolic extract of Paulownia tormentosa fruits, with a potent inhibitory activity against AChE, was subjected to bioactivity-guided fractionation which allowed the identification of some geranylated flavonoids, such as cholinesterase inhibitors, of which the most active resulted to be 6-geranyl-3,3’,5,5’,7-pentahydroxy-4’-methoxyflavane (164), 6-geranyl-3’,5,5’,7-tetrahydroxy-4’-methoxyflavanone (165) and diplacone (166), which were observed to show mixed-type inhibition of human AChE with IC₅₀= 15.6, 22.9 and 7.2 µM, respectively [63]. In addition, the fact that these compounds were also observed to elicit significant BChE inhibition makes them interesting as potential dual inhibitors.

The flavonols present in Sophora flavescens (Fabaceae) were studied for several biological activities relevant for AD. Sophoflavescenol (167), icaritin (168), demethylanhydro-icaritin (169), 8-C-lavandurylkaempferol (170) and kaempferol (171) were all found to be good AChE inhibitors, with IC₅₀ values of 8.37, 6.47, 6.67, 5.16 and 3.31 µM, respectively [64]. Compounds 167-171 were also found to elicit significant BChE and BACE1 inhibition.

The methanol extract from roots of Morus lhou (Moraceae), a polyphenol-rich plant, was found to yield nine flavonoids (172 - 180) of which eight showed AChE inhibition [65]. A new flavone, 5’-geranyl-4’-methoxy-5,7,2’-trihydroxyflavone (172), was identified as the most potent inhibitor (IC₅₀ = 10.95 µM). 5’-geranyl-5,7,2’,4’-tetrahydroxyflavone (173), kuwanon U (174), kuwanon E (175), morusin (176), cyclomorusin (178), neocyclomorusin (179) and kuwanon C (180) were all observed to be moderate AChE inhibitors (IC₅₀ = 16.21 - 36.4 µM) and morusinol (177) was observed to be weakly active (IC₅₀ = 173.49 µM). C-3 prenylated flavones 176, 178, 179 and 180 were found to be noncompetitive inhibitors whereas those unsubstituted at C-3 172-175 were mixed inhibitors. Flavonoids 172 - 180 were also found to inhibit BChE [65].

On the other hand, three potent AChEi were obtained from Broussonetia papyrifera, another plant belonging to the Moraceae family. From the ethanolic extract of the roots of B. papyrifera which was found to elicit cholinesterase inhibitory activity, prenylated flavonols 181 – 183 were isolated and characterized [66]. 8-(1,1-dimethylallyl)-5’-(3-methylbut-2-enyl)-3’,4’,5,7-tetrahydroxyflavonol (181), papyriflavonol (182) and broussoflavonol (183) were observed to inhibit human erythrocyte AChE with IC₅₀ values of 0.82, 3.1 and 2.7 µM, respectively. Compound 181, the most potent, acted as a time-dependent, slow reversible inhibitor.

Isoorientin (184) and isovitexin (185) were identified as the compounds responsible for the AChE inhibition observed in the extracts from flowers and rhizomes of Iris pseudopumila (Iridaceae) from Italy [67]. Compound 184 was observed to be the highest inhibitor with an IC₅₀ = 26.8 µM while 185, lacking the 3’-hydroxy group in ring B, was observed to show an IC₅₀ value of 36.4 µM. Both compounds were also found to have the ability of significantly inhibiting BChE.

On the other hand, a pterocarpan with moderate AChE inhibition was isolated from the polar extract of Zygophyllum eurypterum (Zygophyllaceae) collected in Pakistan. Atricarpan D [(-)-2,9-dimethoxy-4-(5-oxohexyl)pterocarpan] (186) was observed to inhibit AChE with an IC₅₀ = 20.5 µM [68]. Interestingly, three other pterocarpans with similar structure were obtained along with atricarpan D but they were found to be inactive against AChE. Nevertheless, the four pterocarpans were all found to be BChE inhibitors.

A study conducted on AChE and BChE inhibitory activity of coumarins and naphtoquinones obtained from Mansonia gagei (Sterculiaceae) proposed a novel class of cholinesterase inhibitor, mansonones or 1,2-naphtoquinones [69]. The level of cholinesterase inhibition observed in this study seemed to correlate to the presence of a fused pyran ring and a substituent at C-6 being present in the molecule. Mansonone E (187) was observed to be the most active AChE (IC₅₀ = 23.5 µM) and BChE inhibitor.

In several studies published during the period covered in the present review various phenolic compounds with different structural characteristics were reported as AChEi. Some of them are structurally simple such as gallic acid (188, IC₅₀ = 5.85 µM) and ellagic acid (189, IC₅₀ = 45.63 µM) [70]. Hopeahainol A (190), which was identified as a new compound isolated from Hopea hainensis, was observed to elicit a notable AChE inhibition (IC₅₀ = 4.33 µM) with respect to huperzine A (IC₅₀ = 1.6 µM), as a reversible mixed-type inhibitor [71].

The bioassay-guided fractionation of the extract from Terminalia chebula (Combretaceae) fruits allowed the isolation of 1,2,3,4,6-penta-O-galloyl-β-D-glucose (191) which demonstrated to be a significant AChE inhibitor (IC₅₀ = 29.9 µM) [72]. This gallotanin which has been also isolated from other different sources and which is known by its diverse biological activities, was observed to exert good BChE inhibition and potent antioxidant activity (FRAP assay) in this study.

The bioassay-guided extraction of the stem bark of Knema laurina (Myristicaceae) yielded two active fractions (dichloromethane and hexane) which were subjected to chromatographic separation. That latter yielded five alkenyl phenol and salicylic acid derivatives 192 - 196, of which 192 and 193 were new compounds [73]. Compounds 192, 195 and 196, all having salicylic acid moiety, were observed to strongly inhibit AChE with an IC₅₀ = 3.182, 2.172 and 0.573 µM, respectively. Compounds 193 and 194, with no carboxyl moiety, were observed to be good AChE inhibitors (IC₅₀ = 17.224 and 13.114 µM, respectively). These findings suggest that the acidic group is key to good AChE inhibition. It was also observed that anti-AChE activity dramatically decreased when the acidic and the phenolic hydroxy group were methylated. Two catechol alkenyls were isolated from the fruits of Semecarpus anacardium (Anacardiaceae), a species used in Ayurvedic medicine for retarding and treatment of memory loss [74]. Compounds 197 and 198 were identified as active components of the dichloromethane extract through a fractionation guided by the detection of AChE inhibition. Microplate assay revealed that these catechol alkenyls are moderate and weak selective AChEi. Compound 197, with a double bond in the aliphatic chain, was identified as a stronger inhibitor (IC₅₀ = 39.7 µM) with respect to compound 198, with two double bonds in the aliphatic chain (IC₅₀ = 108 µM).

On the other hand, four structurally diverse AChEi were isolated from the polar extract of Nelumbo nucifera (Nelumbonaceae) stamens [75]. Cycloartenol (199), p-hydroxybenzoic acid (200), vanilloloside (201) and nuciferoside (202) were found to elicit good and noncompetitive inhibition against AChE with an IC₅₀ = 11.89, 20.07, 4.55 and 3.2 µM, respectively. In the same study, compounds 199, 200 and 202 were observed to exert moderate BChE inhibition and compounds 199 - 202 were found to show no inhibition against BACE1.

PLANT EXTRACTS, FRACTIONS AND ESSENTIAL OILS WITH AChE INHIBITORY ACTIVITY

Table 1 summarizes the studies published from 2006 to 2012 on plant extracts, fractions and essential oils that have been found to be good AChE inhibitors (IC₅₀ < 500 µg/mL). Those plants included in other recent reviews were omitted [76, 77]. Extracts and fractions under further phytochemical studies that led to the discovery of AChE inhibitors were also omitted. Whenever possible, reference is made to the part of the plant used in each study reported. AChE inhibitory activity is reported in the same way as it was reported by authors and IC₅₀ values were chosen instead of inhibition percentages when both were available.

Table 1.

Plant Extracts, Fractions and Essential Oils with AChE Inhibitory Activity

Family and Botanical Name	Type of Extract (Solvent)	Plant's Parts	AChE Inhibition (%)	IC50	Refs.
Acanthaceae
Andrographis paniculata	H2O:EtOH	Aerial		222.41 µg/ml	[78]
Amaranthaceae
Salsola oppositifolia	Alkaloids	Aerial		70.0 µg/ml	[79]
Salsola soda	Alkaloids	Aerial		64.1 µg/ml	[79]
Salsola tragus	Alkaloids	Aerial		30.2 µg/ml	[79]
Amaryllidaceae
Crinum jagus	MeOH	Leaf	74.25 (42 µg/ml)		[80]
Crinum moorei	50% MeOH PE DCM EtOH	Bulb		21.5 µg/ml 18.9 µg/ml 2.9 µg/ml 22.5 µg/ml	[81]
Nerine undulata	Alkaloids	Bulb		14.3 µg/ml a	[82]
Scadoxus multiflorus	Alkaloids	Bulb		313.5 µg/mla	[82]
Sprekelia formosissima	Alkaloids	Bulb		209.7 µg/mla	[82]
Zephyranthes grandiflora	Alkaloids	Bulb		39.2 µg/mla	[83]
Anacardiaceae
Harpephyllum caffrum	DCM MeOH	Leaf Stem bark Leaf		0.17 mg/ml 0.02 mg/ml 0.12 mg/ml	[84]
Pistacia atlantica	H2O	Leaf		0.87 µg/ml	[85]
Pistacia lentiscos	H2O	Leaf		13.67 µg/ml	[85]
Sclerocarya birrea	DCM MeOH	Young stem Leaf Operculum Young stem		0.15 mg/ml 0.10 mg/ml 0.35 mg/ml 0.47 mg/ml	[84]
Spondias mombin	MeOH	Root bark	64.77 (42 µg/ml)		[80]
Apiaceae
Centella asiatica	H2O:EtOH	Whole plant		106.55 µg/ml	[78]
Apocynaceae
Geissospermum vellosii	Alkaloids	Stem bark		2.9 µg/ml	[86]
Araceae
Colocasia antiquorum	50% MeOH PE DCM	Tuber		7.9 µg/ml 6.4 µg/ml 168.1 µg/ml	[81]
Pinellia ternata	Alkaloids	Tuber		56.2 µg/ml	[87]
Arecaceae
Phoenix dactylifera	Hexane	Seed	52.96 (300 µg/ml)		[88]
Asparagaceae
Leopoldia comosa	Hexane	Bulb		104.9 µg/ml	[89]
Asphodelaceae
Aloe ferox	50% MeOH PE DCM	Leaf		84.0 µg/ml 37.7 µg/ml 62.6 µg/ml	[81]
Asteraceae
Achyrocline tomentosa	Organic	Aerial		0.4847 mg/ml	[90]
Arnica chamissonis ssp. foliosa	MeOH Hexane	Flower		43 µg/ml 29 µg/ml	[91]
Chromolaena tequendamensis	MeOH	Whole plant		359.36 mg/l	[92]
Eupatorium viscidum	Organic	Aerial		0.4792 mg/ml	[90]
Pulicaria stephanocarpa	CHCl3	Leaf	61.43 (0.2 mg/ml)		[93]
Schistocarpha sinforosi	MeOH	Whole plant		145.31 mg/l	[92]
Trichocline reptans	Organic	Aerial		0.1118 mg/ml	[90]
Berberidaceae
Berberis darwinii	MeOH	Stem bark		1.23 µg/ml	[94]
Boraginaceae
Onosma bracteata	MeOH	Leaf	59.73 (250 µg/ml)		[95]
Buddlejaceae
Buddleja salviifolia	DCM:MeOH (1:1)	Whole plant		0.05 mg/ml	[96]
Burseraceae
Boswellia socotranao	CHCl3	Resin	71.21 (0.2 mg/ml)		[93]
Cistaceae
Cistus laurifolius	EtOH	Leaf	80.07 (200 µg/ml)		[97]
Combretaceae
Terminalia bellirica	MeOH	Fruit		14.37 µg/ml	[70]
Convolvulaceae
Evolvulus alsinoides	H2O:EtOH	Whole plant		141.76 µg/ml	[78]
Ipomoea asarifolia	MeOH	Leaf		0.12 µg/ml	[98]
Crassulaceae
Kalanchoe brasiliensis	EtOAc	Leaf		0.16 mg/ml	[98]
Cucurbitaceae
Eureiandra balfourii	MeOH	Tuber	58.61 (0.2 mg/ml)		[93]
Cupressaceae
Juniperus phoenicea	EtOH	Leaf	53.44 (400 µg/ml)		[99]
Juniperus turbinata	Phenolic	Leaf	83.84 (400 µg/ml)		[99]
Ericaceae
Rhododendron yedoense var. poukhanense	80% MeOH	Bark		169.01 µg/ml	[100]
Eucommiaceae
Eucommia ulmoides	H2O	Bark		172 µg/ml	[101]
Euphorbiaceae
Alchornia laxiflora	MeOH	Stem bark	41.12 (42 µg/ml)		[80]
Cephalocroton socotranus	CHCl3	Bark	51.1 (0.2 mg/ml)		[93]
Jatropha curcas	MeOH	Leaf		0.25 mg/ml	[98]
Jatropha gossypiifolia	MeOH	Leaf		0.05 mg/ml	[98]
Fabaceae
Acacia nilotica	H2O	Root		0.079 mg/mlb	[102]
Acacia raddiana	H2O	Bark		33.91 µg/ml	[85]
Cassia obtusifolia	EtOH	Seed		81.6 µg/ml c	[103]
Chamaecrista mimosoides	DCM:MeOH (1:1) H2O	Root		0.03 mg/ml 0.35 mg/ml	[96]
Genista tenera	EtOAc	Aerial	77.0 (70 µg/ml)		[105]
Peltophorum pterocarpum	MeOH	Leaf Stem bark	49.5 (42 µg/ml) 68.85 (42 µg/ml)		[80]
Schotia brachypetala	DCM:MeOH (1:1) H2O	Bark		0.27 mg/ml 0.49 mg/ml	[96]
Senna alata	EtOAc	Leaf		0.08 mg/ml	[98]
Spatholobus suberectus	H2O EtOH	Whole plant		85 µg/ml 9 µg/ml	[104]
Trigonella foenum-graecum	EtOAc Alkaloids	Seed		53.00 µg/ml 9.23 µg/ml	[106]
Gobulariaceae
Globularia alypum	H2O	Root		16.67 µg/ml	[85]
Guttiferaceae
Callophyllum inophyllurn	MeOH	Root bark	56.52 (42 µg/ml)		[80]
Hypericaceae
Hypericum perforatum	MeOH	Whole plant		178 µg/ml	[91]
Illiciaceae
Illicium verum	H2O:EtOH Butanol EtOAc CHCl3 Oil	Fruit		58.67 µg/ml 44.94 µg/ml 83.75 µg/ml 103.03 µg/ml 39.89 µg/ml	[107]
Lamiaceae
Cyclotrichium niveum	EtOAc DCM	Whole plant	83.11 (250 µg/ml) 70.82 (250 µg/ml)		[108]
Hyssopus officinials	Hexane	Whole plant	55.0 (400 µg/ml)		[91]
Lavandula viridis	MeOH	Aerial		244.55 µg/ml	[109]
Marrubium vulgare	Acetone	Aerial	62.70 (25 µg/ml)		[110]
Origanum ehrenbergii	Essential oil	Aerial		0.3 µg/ml	[111]
Origanum majorana	Essential oil	Leaf		36.40 µg/ml	[112]
Origanum syriacum	Essential oil	Aerial		1.7 µg/ml	[111]
Pycnostachys reticulata	50% MeOH EtOH	Leaf		28.8 µg/ml 8.8 µg/ml	[81]
Salvia chionantha	Essential oil	Aerial	56.7 (500 µg/ml)		[113]
Salvia fruticosa	DCM	Whole plant	51.07 (100 µg/ml)		[114]
Salvia leriifolia	Essential oil	Aerial		0.32 µl/ml	[115]
Salvia miltiorrhiza	H2O EtOH	Root		50 µg/ml 5 µg/ml	[104]
Teucrium royleanum	MeOH	Whole plant	52.4 (40 µg/0.2ml)		[116]
Menispermaceae
Stephania pierrei	EtOH	Tuber		5.68 µg/ml	[117]
Tinospora cordifolia	MeOH	Stem		38.36 µg/ml	[118]
Moraceae
Dorstenia gigas	CHCl3	Leaf	65.12 (0.2 mg/ml)		[93]
Ficus religiosa	MeOH	Stem bark		73.69 µg/ml	[118]
Myristicaceae
Myristica fragrans	H2O:EtOH	Seed		133.28 µg/ml	[78]
Embelia ribes	MeOH	Root		23.04 µg/ml	[118]
Orchidaceae
Orchis mascula	MeOH	Root	56.99 (250 µg/ml)		[119]
Paeoniaceae
Paeonia lactiflora	H2O EtOH	Root		20 µg/ml 8 µg/ml	[104]
Paeonia veitchii	H2O EtOH	Root		14 µg/ml 45 µg/ml	[104]
Papaveraceae
Corydalis intermedia	MeOH H2O	Whole plant Tuber Whole plant Tuber	84 (100 µg/ml) 97 (100 µg/ml) 57 (100 µg/ml) 78 (100 µg/ml)		[120]
Papaveraceae
Corydalis solida ssp. laxa	MeOH H2O	Whole plant Tuber Whole plant Tuber	89 (100 µg/ml) 96 (100 µg/ml) 78 (100 µg/ml) 85 (100 µg/ml)		[120]
Corydalis solida ssp. slivenensis	MeOH H2O	Whole plant Tuber Whole plant Tuber	82 (100 µg/ml) 97 (100 µg/ml) 48 (100 µg/ml) 87 (100 µg/ml)		[120]
Phyllantaceae
Emblica officinalis	MeOH	Fruit		29.26 µg/ml	[70]
Pinaceae
Pinus halepensis	EtOH Essential oil	Needle Twig	60.15 (200 µg/ml) 83.91 (200 µg/ml)		[121]
Pinus heldreichii subsp. leucodermis	Essential oil	Needle		51.1 µg/ml	[122]
Pinus nigra subsp. nigra	Essential oil	Needle		94.4 µg/ml	[122]
Pinus nigra subsp. calabrica	Essential oil	Needle		101.5 µg/ml	[122]
Pinus pinaster	Pycnogenol	Bark	63.33 (200 µg/ml)		[121]
Piperaceae
Piper nigrum	EtOH	Fruit		30.67 µg/ml	[117]
Poaceae
Cymbopogon jawarancusa	MeOH	Whole plant	72.36 (250 µg/ml)		[95]
Cymbopogon schoenanthus	Essential oil	Fresh leaf (mountain reg./ desert reg.) Dried leaf (mountain reg./ desert reg.) Dried root (mountain reg./ desert reg.)		0.26 / 0.67 mg/ml 0.44 / 0.52 mg/ml 0.27 / 0.32 mg/ml	[123]
Cymbopogon schoenanthus	Hexane DCM EtOAc MeOH H2O	Shoot (mountain reg./ desert reg.)		0.50 / 0.54 mg/ml 0.57 / 0.30 mg/ml 0.23 / 0.30 mg/ml 0.23 / 0.25 mg/ml 0.46 / 0.04 mg/ml	[124]
Polygonaceae
Fallopia multiflora	H2O EtOH	Root		13 µg/ml 65 µg/ml	[104]
Rheum palmatum	H2O EtOH	Root and Rizhome		32 µg/ml 18 µg/ml	[104]
Ruprechtia apetala	EtOH	Aerial		0.0779 mg/ml	[90]
Portulacaceae
Portulaca oleracea	Alkaloids	Upper part		29.4 µg/ml	[87]
Rhamnaceae
Rhamnus prinoides	H2O	Root		0.201 mg/mlb	[102]
Rosaceae
Leucosidea sericea	PE DCM MeOH PE	Leaf Stem		0.16 mg/ml 0.14 mg/ml 0.24 mg/ml 0.26 mg/ml	[125]
Rubiaceae
Galium odoratum	Hexane	Whole plant	53.1 (400 µg/ml)		[91]
Morinda citrifolia	EtOH CHCl3	Fruit		138.4 µg/ml 78.11 µg/ml	[126]
Morinda lucida	MeOH	Leaf	40.15 (42 µg/ml)		[80]
Rutaceae
Citrus aurantifolia	Essential oil	Leaf		139 µg/ml	[127]
Citrus medica	Essential oil	Peel		171.3 µg/ml	[128]
Ruta graveolens	MeOH Hexane	Whole plant	59.1 (100 µg/ml)	34 µg/ml	[91]
Zanthoxylum coco	Organic	Aerial		0.1579 mg/ml	[90]
Solanaceae
Solanum leucocarpum	MeOH	Whole plant		204.59 mg/l	[92]
Withania somnifera	MeOH	Root		33.38 µg/ml	[118]
Witheringia coccoloboides	MeOH	Whole plant		220.68 mg/l	[92]
Valerianaceae
Nardostachys jatamansi	H2O:EtOH MeOH	Rhizome		130.11 µg/ml 47.21 µg/ml	[78] [118]
Zingiberaceae
Kaempfera parviflora	EtOH	Rhizome		20.64 µg/ml	[117]

DCM: dichloromethane; MeOH: methanol; EtOH: ethanol; PE: petroleum ether; EtOAc: ethyl acetate

^aHuman blood AChE.

^bBovine erythrocyte AChE.

^cMouse brain homogenized.

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CONFLICT OF INTEREST

The author(s) confirm that this article content has no conflict of interest.