Splenic B cells were co-cultured with in vitro skewed Th17 cells derived from OTII (n = 12) or IL-17−/− OTII (n = 9) mice in the presence and absence of OVA323-339 peptides for 6 days. The levels of IgA, IgE, IgG1, IgG2b, IgG2c, IgG3 and IL-17 in the culture supernatants were measured by ELISA. Data show the mean ± SEM. *p<0.05, **p<0.01 vs. Medium; and ††p<0.01 vs. WT OTII Th17 cells. Similar results were obtained in two independent experiments.