Table 6. ANOVA sheet of PEGylated nanoparticles bearing temozolomide on percent drug entrapment.
| Table 6A | ||||||
|---|---|---|---|---|---|---|
| Response―PDE | ANOVA for Response Surface Quadratic Model | Analysis of variance table [Partial sum of squares-Type III] | ||||||
| Source | Sum of Squares | df | Mean Square | F-Value | p-value Prob > F |
|
| Model |
9876.487 |
14 |
705.4634 |
75.1158 |
< 0.0001 |
Significant |
| A-Drug |
3816.122 |
1 |
3816.122 |
406.3302 |
< 0.0001 |
|
| B-PLGA |
3168.176 |
1 |
3168.176 |
337.3387 |
< 0.0001 |
|
| C-PVA |
55.89636 |
1 |
55.89636 |
5.951691 |
0.0174 |
|
| D-ST |
59.09482 |
1 |
59.09482 |
6.292254 |
0.0146 |
|
| AB |
785.5875 |
1 |
785.5875 |
83.6472 |
< 0.0001 |
|
| AC |
1.1236 |
1 |
1.1236 |
0.119638 |
0.7305 |
|
| AD |
2.1025 |
1 |
2.1025 |
0.223868 |
0.6376 |
|
| BC |
8.400336 |
1 |
8.400336 |
0.894445 |
0.3477 |
|
| BD |
0.001736 |
1 |
0.001736 |
0.000185 |
0.9892 |
|
| CD |
0.298844 |
1 |
0.298844 |
0.03182 |
0.859 |
|
| A^2 |
1082.655 |
1 |
1082.655 |
115.2782 |
< 0.0001 |
|
| B^2 |
827.0542 |
1 |
827.0542 |
88.06246 |
< 0.0001 |
|
| C^2 |
4.171924 |
1 |
4.171924 |
0.444215 |
0.5074 |
|
| D^2 |
4.849833 |
1 |
4.849833 |
0.516397 |
0.4749 |
|
| Residual |
629.2424 |
67 |
9.391677 |
|
|
|
| Lack of Fit |
628.4103 |
66 |
9.521369 |
11.44326 |
0.2315 |
not significant |
| Pure Error |
0.83205 |
1 |
0.83205 |
|
|
|
| Cor Total | 10505.73 | 81 | ||||
| Table 6B | |||
|---|---|---|---|
| Standard deviation | 3.064584 | R-Squared | 0.940105 |
| Mean |
61.81098 |
Adj R-Squared |
0.927589 |
| C.V. % |
4.957994 |
Pred R-Squared |
0.912094 |
| PRESS | 923.5207 | Adeq Precision | 27.6636 |
| Table 6C | ||||||
|---|---|---|---|---|---|---|
| Factor | Coefficient |
Standard error | 95% CI low | 95% CI high | VIF | |
| Estimate | Df | |||||
| Intercept |
71.92667 |
1 |
0.969107 |
69.99232 |
73.86101 |
|
| A-Drug |
8.406481 |
1 |
0.417037 |
7.574072 |
9.238891 |
1 |
| B-PLGA |
7.65963 |
1 |
0.417037 |
6.82722 |
8.492039 |
1 |
| C-PVA |
1.017407 |
1 |
0.417037 |
0.184998 |
1.849817 |
1 |
| D-ST |
-1.04611 |
1 |
0.417037 |
-1.87852 |
-0.2137 |
1 |
| AB |
4.671389 |
1 |
0.510764 |
3.6519 |
5.690878 |
1 |
| AC |
0.176667 |
1 |
0.510764 |
-0.84282 |
1.196156 |
1 |
| AD |
-0.24167 |
1 |
0.510764 |
-1.26116 |
0.777823 |
1 |
| BC |
-0.48306 |
1 |
0.510764 |
-1.50254 |
0.536434 |
1 |
| BD |
0.006944 |
1 |
0.510764 |
-1.01254 |
1.026434 |
1 |
| CD |
-0.09111 |
1 |
0.510764 |
-1.1106 |
0.928378 |
1 |
| A^2 |
-7.66883 |
1 |
0.714258 |
-9.09449 |
-6.24316 |
1.001626 |
| B^2 |
-6.70272 |
1 |
0.714258 |
-8.12838 |
-5.27705 |
1.001626 |
| C^2 |
-0.47605 |
1 |
0.714258 |
-1.90172 |
0.949617 |
1.001626 |
| D^2 | -0.51327 | 1 | 0.714258 | -1.93894 | 0.912394 | 1.001626 |
Final Equation in terms of Coded factors: PDE = 71.92+ 8.40A + 7.65B + 1.01C – 1.04D + 4.67AB + 0.17AC - 0.24AD – 0.48BC + 0.01BD – 0.09CD – 7.66A2 – 6.70B2 – 0.47C2 – 0.51D2…. Eqn. 3.