Table 2.

Probable pathways of communication between the aminoacylation and the INS domains in Ec ProRS as identified using STCA^a

Pathways	Parameters	Residue Networks	Cost (Å)
C443→K279 I (8 residues)	Cij ≥ 0.8 $\mathrm{\Delta }{G}_i^{\text{stat}}=0.5$ CDCij = 0.4 $D_{ij}^o=8.0$	C443→F415→T199→D198→E234 →N389→H302→K279	43.9 (0.62,0.51,60)
II (9 residues)	Cij ≥ 0.8 $\mathrm{\Delta }{G}_i^{\text{stat}}=0.6$ CDCij = 0.4 $D_{ij}^o=8.0$	C443→F415→T199→D 198→E234→ N305→L304→L281→K279	46.6 (0.66,0.59, 0.62)
R450→K279 III (10 residues)	Cij ≥ 0.8 $\mathrm{\Delta }{G}_i^{\text{stat}}=0.6$ CDCij = 0.4 $D_{ij}^o=8.0$	R450→V411→G412→E209→D198 →E234→N305→L304→L281→K279	51.5 (0.47,0.60, 0.63)
IV (10 residues)	Cij ≥ 0.8 $\mathrm{\Delta }{G}_i^{\text{stat}}=0.5$ CDCij = 0.4 $D_{ij}^o=8.0$	R450→I409→E218→D219→N232→ E234→N305→L304→L281→K279	53.3 (0.74,0.65, 0.64)

^aC_ij, correlation coefficient of residue-residue fluctuations; $\mathrm{\Delta }{G}_i^{\text{stat}}$, evolutionary conservation constant; $\mathrm{\Delta }\mathrm{\Delta }{G}_i^{\text{stat}}$, evolutionary coupling constant; ${\mathrm{CDC}}_{ij}=\mathrm{\Delta }\mathrm{\Delta }{\text{G}}_{ij}^{\text{stat}}\times C_{ij}$, coevolutionary dynamic coupling constant; $D_{ij}^{\text{o}}$, distance cutoff. Numbers in parentheses in column 4 represent average C_ij between adjacent residues in a given path obtained from the dynamic cross-correlation matrix C for the three replica simulations. The residues in bold represent the terminal residues, all but residues shown in italics are conserved residues; residues in italics represent the co-evolved residues. The underlined residues were chosen for mutational studies.