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Merged images of DIGE analysis after reciprocal labeling of nuclear extracts from normal and from HCl-irritated rat bladder (day 8). Normal extract was labeled with Cy5 (left) and Cy3 (right) and HCl-treated extract was labeled with Cy3 (left) and Cy5 (right). Computer image analysis selected four spots (A1–A4) as overexpressed and one (A5) as underexpressed in HCl-irritated bladder compared to normal rat bladder. See text for protein ID details.
