A. ΔF/F traces from PERin axons of E564-Gal4/UAS-GCaMP5; UAS-GCaMP5 for 4 individual flies (black lines), with leg movement indicated by shaded rectangles. ΔF/F traces of the same flies with immobilized legs are shown below (red lines). Calcium transients seen in moving animals are correlated with movement and are abolished in immobilized flies.
B. ΔF/F traces from PERin axons of E564-Gal4, UAS-mCD8::GFP fliesfor 2 individual flies (black lines), with leg movement indicated by shaded rectangles.
C. Correlation values between movement and ΔF/F values in freely moving E564-Gal4/UAS-GCaMP5; UAS-GCaMP5 flies (n=10), shuffled data (n=90), and freely moving E564-Gal4, UAS-mCD8::GFP flies (n=4). Data is mean ± SEM, two-sided t-test, ***P<0.001.