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. Author manuscript; available in PMC: 2013 Dec 5.
Published in final edited form as: Virology. 2012 Sep 1;434(1):43–49. doi: 10.1016/j.virol.2012.08.004

Fig 3. Western blot analysis of N protein expression.

Fig 3

HUVEC or THP-1 cells were infected with HTNV, MJNV, TPMV or PHV (MOI of 1.0). Cells were lysed at 1, 3, 6 days postinfection as indicated. Total protein levels were determined, and an equivalent amount of whole-cell lysate was separated by 10% SDS-polyacrylamide gel electrophoresis. Proteins were detected by Western blotting using anti-nucleocapsid monoclonal mouse or polyclonal rat antibody, followed by HRP-conjugated species-specific secondary antibodies. Anti-GAPDH monoclonal antibody was used as a loading control.