Abstract
Enteroviruses can be made to adsorb or to pass through membrane filters by manipulation of the suspending medium. Salts facilitate virus adsorption, but membrane-coating components (MCC) interfere. Because cells release MCC into the culture medium during viral growth, MCC must be removed before virus can be adsorbed to membranes. Adsorbed virus can be eluted with diluents containing MCC (cell extracts or serum) or agents that reduce surface tension (sodium lauryl sulfate). By membrane adsorption and elution, enteroviruses can be readily concentrated and quantitatively recovered from crude virus harvests.
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Selected References
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- Hsiung G. D. Use of ultrafiltration for animal virus grouping. Bacteriol Rev. 1965 Dec;29(4):477–486. doi: 10.1128/br.29.4.477-486.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WALLIS C., MELNICK J. L. Magnesium chloride enhancement of cell susceptibility to poliovirus. Virology. 1962 Feb;16:122–132. doi: 10.1016/0042-6822(62)90287-8. [DOI] [PubMed] [Google Scholar]
- WALLIS C., MELNICK J. L. PHOTODYNAMIC INACTIVATION OF POLIOVIRUS. Virology. 1963 Nov;21:332–341. doi: 10.1016/0042-6822(63)90194-6. [DOI] [PubMed] [Google Scholar]
- WALLIS C., MELNICK J. L. Suppression of adventitious agents in monkey kidney cultures. Tex Rep Biol Med. 1962;20:465–475. [PubMed] [Google Scholar]
- WARREN J., WEIL M. L. Purification of certain viruses by use of protamine sulfate. Proc Soc Exp Biol Med. 1949 Dec;72(3):662–664. doi: 10.3181/00379727-72-17535. [DOI] [PubMed] [Google Scholar]