Abstract
It is known that plaque formation by some rhinoviruses is greatly enhanced by increasing the concentration of MgCl2. The mechanism of this action was studied by investigating the effects of MgCl2 on rhinovirus HGP adsorption, growth, clumping, thermal stability, and cell susceptibility to viral cytopathic effect. The latent period was at most 7 hr, whether virus was propagated in cells maintained in 0.8 or 30 mm MgCl2, but virus release was 8- to 310-fold greater in the presence of 30mm MgCl2, depending on initial multiplicity. Intracellular virus content appeared unaffected by Mg++ and reached maximal yield (plateau phase) at about 10 hr. Viral adsorption was increased when cells were maintained in 30mm Mg++. It is likely that the two effects of magnesium, enhanced adsorption and increased virus release, both contribute to enhancement of plaque formation.
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Selected References
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