Abstract
Messenger ribonucleic acid (mRNA) preparations from T4-infected cells formed in the presence or absence of putrescine have been characterized and compared. Hybrid competition experiments indicate that these mRNA molecules are derived from distinct genetic loci. The results are consistent with the hypothesis that putrescine might control differential transcription of the phage genome during morphogenesis. The data are also in accord with previously observed changes in the population of mRNA formed at different times after infection.
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Selected References
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- Bautz E. K., Kasai T., Reilly E., Bautz F. A. Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1966 May;55(5):1081–1088. doi: 10.1073/pnas.55.5.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bernardi G. Chromatography of nucleic acids on hydroxyapatite. Nature. 1965 May 22;206(4986):779–783. doi: 10.1038/206779a0. [DOI] [PubMed] [Google Scholar]
- Geiduschek E. P., Snyder L., Colvill A. J., Sarnat M. Selective synthesis of T-even bacteriophage early messenger in vitro. J Mol Biol. 1966 Aug;19(2):541–547. doi: 10.1016/s0022-2836(66)80021-9. [DOI] [PubMed] [Google Scholar]
- Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
- HALL B. D., NYGAARD A. P., GREEN M. H. CONTROL OF T2-SPECIFIC RNA SYNTHESIS. J Mol Biol. 1964 Jul;9:143–153. doi: 10.1016/s0022-2836(64)80096-6. [DOI] [PubMed] [Google Scholar]
- HERSHEY A. D., GOLDBERG E., BURGI E., INGRAHAM L. Local denaturation of DNA by shearing forces and by heat. J Mol Biol. 1963 Mar;6:230–243. doi: 10.1016/s0022-2836(63)80072-8. [DOI] [PubMed] [Google Scholar]
- KANO-SUEOKA T., SPIEGELMAN S. Evidence for a nonrandom reading of the genome. Proc Natl Acad Sci U S A. 1962 Nov 15;48:1942–1949. doi: 10.1073/pnas.48.11.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAHLER H. R., MEHROTRA B. D. Dependence of deoxyribonucleic-amine interactions on deoxyribonucleic acid composition. Biochim Biophys Acta. 1962 Jan 22;55:252–254. doi: 10.1016/0006-3002(62)90965-4. [DOI] [PubMed] [Google Scholar]
- MEHROTRA B. D., MAHLER H. R. STUDIES ON POLYNUCLEOTIDE-SMALL MOLECULE INTERACTIONS. 3. THE EFFECT OF DIAMINES ON HELICAL POLYNUCLEOTIDES. Biochim Biophys Acta. 1964 Sep 11;91:78–91. [PubMed] [Google Scholar]
- MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
- Matthews C. K. Deoxyribonucleic acid metabolism and virus-induced enzyme synthesis in a thymine-requiring bacterium infected by a thymine-requiring bacteriophage. Biochemistry. 1966 Jun;5(6):2092–2100. doi: 10.1021/bi00870a042. [DOI] [PubMed] [Google Scholar]
- SEKIGUCHI M., COHEN S. S. THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS. II. SYNTHESIS OF PHAGE-INDUCED RNA AND SEQUENTIAL ENZYME PRODUCTION. J Mol Biol. 1964 May;8:638–659. doi: 10.1016/s0022-2836(64)80114-5. [DOI] [PubMed] [Google Scholar]
- Shalitin C., Sarid S. Differential effect of polyamines on T4 morphogenesis. J Virol. 1967 Jun;1(3):559–568. doi: 10.1128/jvi.1.3.559-568.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terzaghi E., Okada Y., Streisinger G., Emrich J., Inouye M., Tsugita A. Change of a sequence of amino acids in phage T4 lysozyme by acridine-induced mutations. Proc Natl Acad Sci U S A. 1966 Aug;56(2):500–507. doi: 10.1073/pnas.56.2.500. [DOI] [PMC free article] [PubMed] [Google Scholar]