Abstract
The ribonucleic acid (RNA) product resulting from annealing R17 RNA with denatured replicative form or replicative intermediate could be divided into two distinct types of RNA by precipitation in 1.5 m NaCl. The RNA found in the salt supernatant fluid was resistant to digestion by ribonuclease, had a sedimentation coefficient of 15S, and displayed a sharp thermal transition. The RNA in the salt supernatant fluid appeared to be identical to replicative form. The RNA found in the salt precipitate was resistant to digestion by ribonuclease, but possessed both single- and double-stranded characteristics. The RNA sedimented as a broad band in a sucrose gradient, with a sedimentation coefficient of 15S, and displayed a melting transition characteristic of a mixture of single- and double-stranded RNA. Mild ribonuclease digestion of the salt-precipitable RNA produced a ribonuclease-resistant material with sedimentation properties identical to the RNA found in the salt supernatant fluid.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Doty P., Boedtker H., Fresco J. R., Haselkorn R., Litt M. SECONDARY STRUCTURE IN RIBONUCLEIC ACIDS. Proc Natl Acad Sci U S A. 1959 Apr;45(4):482–499. doi: 10.1073/pnas.45.4.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FENWICK M. L., ERIKSON R. L., FRANKLIN R. M. REPLICATION OF THE RNA OF BACTERIOPHAGE R17. Science. 1964 Oct 23;146(3643):527–530. doi: 10.1126/science.146.3643.527. [DOI] [PubMed] [Google Scholar]
- Francke B., Hofschneider P. H. Infectious nucleic acids of e. Coli bacteriophages, ix. Sedimentation constants and strand integrity of infectious m12 phage replicative-form RNA. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1883–1890. doi: 10.1073/pnas.56.6.1883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franklin R. M., Granboulan N. Ultrastructure of Escherichia coli cells infected with bacteriophage R17. J Bacteriol. 1966 Feb;91(2):834–848. doi: 10.1128/jb.91.2.834-848.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franklin R. M. Replication of bacteriophage ribonucleic acid: some physical properties of single-stranded, double-stranded, and branched viral ribonucleic acid. J Virol. 1967 Feb;1(1):64–75. doi: 10.1128/jvi.1.1.64-75.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franklin R. M. Replication of bacteriophage ribonucleic acid: some properties of native and denatured replicative intermediate. J Virol. 1967 Jun;1(3):514–522. doi: 10.1128/jvi.1.3.514-522.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iglewski W. J., Franklin R. M. Denaturation and renaturation of viral ribonucleic acid. I. Annealing R17 ribonucleic acid with denatured replicative form or with denatured replicative intermediate. J Virol. 1967 Aug;1(4):793–803. doi: 10.1128/jvi.1.4.793-803.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISSMANN C., BILLETER M. A., SCHNEIDER M. C., KNIGHT C. A., OCHOA S. REPLICATION OF VIRAL RNA. VI. NUCLEOTIDE COMPOSITION OF THE REPLICATIVE FORM OF TOBACCO MOSAIC VIRUS RNA AND OF ITS COMPONENT STRANDS. Proc Natl Acad Sci U S A. 1965 Mar;53:653–656. doi: 10.1073/pnas.53.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]