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. 1967 Oct;1(5):980–987. doi: 10.1128/jvi.1.5.980-987.1967

Physicochemical Characterization of Adeno-associated Satellite Virus Type 4 and Its Nucleic Acid

Wade P Parks 1,2, Maurice Green 1,2,1, Magdalena Piña 1,2, Joseph L Melnick 1,2
PMCID: PMC375377  PMID: 4987175

Abstract

Based on protein, deoxyribonucleic acid (DNA), and phosphorus analyses, adeno-associated satellite virus type 4 has a DNA content of 26.5%, significantly larger than type 1 which contains 18.9% DNA. Type 4 DNA contains 58 to 62% guanine + cytosine, as calculated from thermal denaturation temperature and buoyant density measurements. Simian adenovirus SV15, which serves as a helper virus for type 4 satellite, contains 13.7% DNA with a guanine + cytosine content of 55 to 57%. The DNA extracted from type 4 satellite virus is a linear, double-stranded molecule, as shown by thermal denaturation temperature profile measurements and by electron microscopy. The contour length and sedimentation coefficient of type 4 DNA indicate a molecular weight of 3.0 × 106 daltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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