Abstract
A simple method is described for the isolation of bovine papilloma virus and its deoxyribonucleic acid (DNA). As found with other representatives of this virus group, this DNA preparation contains two components, I and II, as shown by sedimentation and electron microscopic studies. Component I is a fast-sedimenting, twisted, circular DNA molecule and represents usually 70 to 90% of the DNA in the mixture. The direction of the twist in the superstructure is right-handed. Component II originates from I by one or more single-strand breaks and is the “relaxed” circular from of the viral DNA.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CRAWFORD L. V., CRAWFORD E. M. A COMPARATIVE STUDY OF POLYOMA AND PAPILLOMA VIRUSES. Virology. 1963 Oct;21:258–263. doi: 10.1016/0042-6822(63)90265-4. [DOI] [PubMed] [Google Scholar]
- Crawford L. V. A study of human papilloma virus DNA. J Mol Biol. 1965 Sep;13(2):362–372. doi: 10.1016/s0022-2836(65)80103-6. [DOI] [PubMed] [Google Scholar]
- Kroon A. M., Borst P., Bruggen E. F., Ruttenberg G. J. Mitochondrial DNA from sheep heart. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1836–1843. doi: 10.1073/pnas.56.6.1836. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lang D., Bujard H., Wolff B., Russell D. Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths. J Mol Biol. 1967 Jan 28;23(2):163–181. doi: 10.1016/s0022-2836(67)80024-x. [DOI] [PubMed] [Google Scholar]
- Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VINOGRAD J., BRUNER R., KENT R., WEIGLE J. Band-centrifugation of macromolecules and viruses in self-generating density gradients. Proc Natl Acad Sci U S A. 1963 Jun;49:902–910. doi: 10.1073/pnas.49.6.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vinograd J., Lebowitz J., Radloff R., Watson R., Laipis P. The twisted circular form of polyoma viral DNA. Proc Natl Acad Sci U S A. 1965 May;53(5):1104–1111. doi: 10.1073/pnas.53.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIL R. A quantitative assay for a subviral infective agent related to polyoma virus. Virology. 1961 May;14:46–53. doi: 10.1016/0042-6822(61)90130-1. [DOI] [PubMed] [Google Scholar]