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. 1967 Dec;1(6):1203–1206. doi: 10.1128/jvi.1.6.1203-1206.1967

Replication of Herpes-Type Virus in a Burkitt Lymphoma Cell Line

Yorio Hinuma a,1, Mitsuru Konn a, Junji Yamaguchi a, James T Grace Jr a
PMCID: PMC375410  PMID: 4912245

Abstract

Replication of the herpes-type virus in the P3HR-1 Burkitt lymphoma cell line was studied. The cell cultures with 106 viable cells/ml were incubated at 33 C for 15 days. The amount of virus in both the cell and fluid portions of the cultures was determined by the loop-drop particle-counting procedure with electron microscopy. An apparent growth curve of the virus was constructed. The maximal cell-associated virus, 1010 virus particles in an 80-ml culture, was observed after 9 days of incubation. The maximal extracellular virus, 2.5 × 109 particles per culture, was observed at the 12th day. About 10% of the released virus particles were enveloped. Under these conditions, there was little or no cell multiplication, but the percentage of immunofluorescent cells reactive to a selected human serum (probably indicating the presence of virus in the cells) increased to a maximum of 50% at the 9th day.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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