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. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968

Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis

A J Shatkin a, J D Sipe a, P Loh a,1
PMCID: PMC375428  PMID: 5723704

Abstract

Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 × 106 daltons, respectively, or a total of 15 × 106 daltons.

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Selected References

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