Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1968 Oct;2(10):999–1005. doi: 10.1128/jvi.2.10.999-1005.1968

Genetic Relatedness Studies with Adenovirus-associated Viruses

James A Rose 1, M David Hoggan 1, Frank Koczot 1, Aaron J Shatkin 1
PMCID: PMC375430  PMID: 5739847

Abstract

Adenovirus-associated viruses (AAV) contain double-stranded deoxyribonucleic acid (DNA). DNA from each of the four AAV serotypes was used as template for the in vitro synthesis of complementary 3H-ribonucleic acids(RNA). An estimation of genetic interrelatedness was made on the basis of hybridization reactions between synthetic AAV RNA and AAV DNA. Heterologous reactions were 27 to 37% of homologous reactions, suggesting that the AAV serotypes are related to about the same extent. AAV-1 synthetic RNA was also reacted with DNA from helper adenovirus types 2, 7, and SV15. Very low levels of RNA binding were observed, but it is not likely that these reactions represent AAV-adenovirus genetic relatedness.

Full text

PDF
999

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATCHISON R. W., CASTO B. C., HAMMON W. M. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES. Science. 1965 Aug 13;149(3685):754–756. doi: 10.1126/science.149.3685.754. [DOI] [PubMed] [Google Scholar]
  2. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  3. Baum S. G., Reich P. R., Hybner C. J., Rowe W. P., Weissman S. M. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509–1515. doi: 10.1073/pnas.56.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blacklow N. R., Hoggan M. D., Rowe W. P. Isolation of adenovirus-associated viruses from man. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1410–1415. doi: 10.1073/pnas.58.4.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blacklow N. R., Hoggan M. D., Rowe W. P. Serologic evidence for human infection with adenovirus-associated viruses. J Natl Cancer Inst. 1968 Feb;40(2):319–327. [PubMed] [Google Scholar]
  6. CHAMBERLIN M., BERG P. Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 1962 Jan 15;48:81–94. doi: 10.1073/pnas.48.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen S. N., Maitra U., Hurwitz J. Role of DNA in RNA synthesis. XI. Selective transcription of gamma DNA segments in vitro by RNA polymerase of Escherichia coli. J Mol Biol. 1967 May 28;26(1):19–38. doi: 10.1016/0022-2836(67)90258-6. [DOI] [PubMed] [Google Scholar]
  8. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  9. Geiduschek E. P., Snyder L., Colvill A. J., Sarnat M. Selective synthesis of T-even bacteriophage early messenger in vitro. J Mol Biol. 1966 Aug;19(2):541–547. doi: 10.1016/s0022-2836(66)80021-9. [DOI] [PubMed] [Google Scholar]
  10. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  11. Henry P., Black P. H., Oxman M. N., Weissman S. M. Stimulation of DNA synthesis in mouse cell line 3T3 by Simian virus 40. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1170–1176. doi: 10.1073/pnas.56.4.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoggan M. D., Blacklow N. R., Rowe W. P. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467–1474. doi: 10.1073/pnas.55.6.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  14. Parks W. P., Green M., Piña M., Melnick J. L. Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid. J Virol. 1967 Oct;1(5):980–987. doi: 10.1128/jvi.1.5.980-987.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parks W. P., Melnick J. L., Rongey R., Mayor H. D. Physical assay and growth cycle studies of a defective adeno-satellite virus. J Virol. 1967 Feb;1(1):171–180. doi: 10.1128/jvi.1.1.171-180.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reich P. R., Somerson N. L., Hybner C. J., Chanock R. M., Weissman S. M. Genetic differentiation by nucleic Acid homology I. Relationships among Mycoplasma species of man. J Bacteriol. 1966 Aug;92(2):302–310. doi: 10.1128/jb.92.2.302-310.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reich P. R., Somerson N. L., Rose J. A., Weissman S. M. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J Bacteriol. 1966 Jan;91(1):153–160. doi: 10.1128/jb.91.1.153-160.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose J. A., Hoggan M. D., Shatkin A. J. Nucleic acid from an adeno-associated virus: chemical and physical studies. Proc Natl Acad Sci U S A. 1966 Jul;56(1):86–92. doi: 10.1073/pnas.56.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  20. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  21. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  22. Smith K. O., Gehle W. D., Thiel J. F. Properties of a small virus associated with adenovirus type 4. J Immunol. 1966 Dec;97(6):754–766. [PubMed] [Google Scholar]
  23. Somerson N. L., Reich P. R., Walls B. E., Chanock R. M., Weissman S. M. Genetic Differentiation by Nucleic Acid Homology II. Genotypic Variations Within Two Mycoplasma Species. J Bacteriol. 1966 Aug;92(2):311–317. doi: 10.1128/jb.92.2.311-317.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES