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. 1968 Oct;2(10):1107–1114. doi: 10.1128/jvi.2.10.1107-1114.1968

Escherichia coli K Bacteriophages I. Isolation and Introductory Characterization of Five Escherichia coli K Bacteriophages

Stephan Stirm a,1
PMCID: PMC375443  PMID: 5723715

Abstract

A set of five Escherichia coli K phages has been isolated. These phages are adsorbed to and lyse the capsular forms of the host bacteria, whereas their spontaneous, acapsular mutants are not affected. All host strains are heavily encapsulated test strains for E. coli K antigens of the thermostable A type and they readily segregate acapsular mutants. In four of the phage-host systems, all secondary growth obtained was found to be acapsular. When tested for host-range mutants on 38 strains of E. coli and Klebsiella, less than one mutant per 105 plaque-forming units was found. No cross-reacting neutralizing antibodies were obtained when rabbits were immunized with the K phages. The latent periods (between 16 and 30 min) and average burst sizes (between 145 and 580) were determined by one-step growth experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer M. E., Anderson T. F. The surface structure of Escherichia coli. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1592–1599. doi: 10.1073/pnas.54.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beumer J., Beumer-Jochmans M. P., Dirkx J., Dekegel D. Etat actuel des connaissances concernant la nature et la localisation des récepteurs des bactériophages dans la parol cellulaire des Shigella et des Escherichia. Bull Acad R Med Belg. 1965;5(9):749–790. [PubMed] [Google Scholar]
  3. Beumer J., Beumer-Jochmans M. P., Dirkx J., Dekegel D. Localisation des récepteurs de phages chez des souches de Shigella et d'Escherichia. Ann Inst Pasteur (Paris) 1966 May;110(5):727–736. [PubMed] [Google Scholar]
  4. Bradley D. E. The morphology and physiology of bacteriophages as revealed by the electron microscope. J R Microsc Soc. 1965 Sep;84(3):257–316. [PubMed] [Google Scholar]
  5. Humphries J. C. Enzymic Activity of Bacteriophage-Culture Lysates: I. A Capsule Lysin Active against Klebsiella pneumoniae Type A. J Bacteriol. 1948 Nov;56(5):683–693. [PMC free article] [PubMed] [Google Scholar]
  6. Hungerer D., Jann K., Jann B., Orskov F., Orskov I. Immunochemistry of K antigens of Escherichia coli. 4. The K antigen of E. coli O 9:K30:H12. Eur J Biochem. 1967 Jul;2(1):115–126. doi: 10.1111/j.1432-1033.1967.tb00115.x. [DOI] [PubMed] [Google Scholar]
  7. JESAITIS M. A., GOEBEL W. F. Lysis of T4 phage by the specific lipocarbohydrate of phase II Shigella sonnei. J Exp Med. 1955 Dec 1;102(6):733–752. doi: 10.1084/jem.102.6.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jann K., Jann B., Orskov F., Orskov I., Westphal O. Immunchemische Untersuchungen an K-Antigenen von Escherichia Coli. II. Das K-Antigen von E. coli 08:K42(A):H-. Biochem Z. 1965 Jun 3;342(1):1–22. [PubMed] [Google Scholar]
  9. Jarvis F. G., Mesenko M. T., Martin D. G., Perrine T. D. Physiochemical properties of the Vi antigen before and after mild alkaline hydrolysis. J Bacteriol. 1967 Nov;94(5):1406–1410. doi: 10.1128/jb.94.5.1406-1410.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLENBERGER E., BOLLE A., BOYDELATOUR E., EPSTEIN R. H., FRANKLIN N. C., JERNE N. K., REALE SCAFATI A., SECHAUD J. FUNCTIONS AND PROPERTIES RELATED TO THE TAIL FIBERS OF BACTERIOPHAGE T4. Virology. 1965 Jul;26:419–440. doi: 10.1016/0042-6822(65)90006-1. [DOI] [PubMed] [Google Scholar]
  11. Kwiatkowski B. The structure of Vi-phage II. Acta Microbiol Pol. 1966;15(1):23–26. [PubMed] [Google Scholar]
  12. Lindberg A. A. Studies of a receptor for felix O-1 phage in Salmonella minnesota. J Gen Microbiol. 1967 Aug;48(2):225–233. doi: 10.1099/00221287-48-2-225. [DOI] [PubMed] [Google Scholar]
  13. ORSKOV I., ORSKOV F., SOJKA W. J., LEACH J. M. Simultaneous occurrence of E. coli B and Lantigens in strains from diseased swine. Influence of cultivation temperature. Two new E. coli Kantigens: K 87 and K 88. Acta Pathol Microbiol Scand. 1961;53:404–422. [PubMed] [Google Scholar]
  14. Rakieten M. L., Eggerth A. H., Rakieten T. L. Studies with Bacteriophages Active against Mucoid Strains of Bacteria. J Bacteriol. 1940 Oct;40(4):529–545. doi: 10.1128/jb.40.4.529-545.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shands J. W., Jr, Graham J. A., Nath K. The morphologic structure of isolated bacterial lipopolysaccharide. J Mol Biol. 1967 Apr 14;25(1):15–21. doi: 10.1016/0022-2836(67)90275-6. [DOI] [PubMed] [Google Scholar]
  16. Simon L. D., Anderson T. F. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. II. Structure and function of the baseplate. Virology. 1967 Jun;32(2):298–305. doi: 10.1016/0042-6822(67)90278-4. [DOI] [PubMed] [Google Scholar]
  17. Sutherland I. W. Phage-induced fucosidases hydrolysing the exopolysaccharide of Klebsiella arogenes type 54 [A3(S1)]. Biochem J. 1967 Jul;104(1):278–285. doi: 10.1042/bj1040278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TAYLOR K. A STUDY ON THE LOSS OF THE RECEPTOR ACTIVITY OF VI-POLYSACCHARIDE DURING INCUBATION WITH VI-PHAGE II. Acta Biochim Pol. 1965;12:157–166. [PubMed] [Google Scholar]
  19. TAYLOR K., KWIATKOWSKI B. Adsorption of Vi-phage II on the Vi-receptor coated erythrocyte membranes, examined in the electron microscope. Acta Microbiol Pol. 1963;12:107–112. [PubMed] [Google Scholar]
  20. TAYLOR K., TAYLOR A. Estimation of Vi-receptor activity. Acta Microbiol Pol. 1963;12:97–106. [PubMed] [Google Scholar]
  21. Taylor K. Physical and chemical changes of Vi-polysaccharide due to Vi-phage II action. Acta Biochim Pol. 1966;13(1):97–106. [PubMed] [Google Scholar]
  22. WILDY P., ANDERSON T. F. CLUMPING OF SUSCEPTIBLE BACTERIA BY BACTERIOPHAGE TAIL FIBRES. J Gen Microbiol. 1964 Feb;34:273–283. doi: 10.1099/00221287-34-2-273. [DOI] [PubMed] [Google Scholar]

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