Abstract
A virus resembling type C murine leukemia viruses, which is associated with transplantable hamster tumors, was partially characterized with respect to certain biological, biophysical, and cytochemical features. As determined by electron microscopy, high concentrations of the virus appeared in the blood plasmas of tumor-bearing hamsters. Hamsters inoculated with virus concentrates did not show gross evidence of disease, and preliminary attempts to infect various hamster cells in tissue culture were unsuccessful. A line of cells from a virus-containing tumor which had been established in tissue culture released large numbers of the virus into the supernatant fluids by budding. The buoyant density peak of virus concentrates in potassium tartrate density gradients was 1.13 g/cm3 by ultraviolet absorption and electron microscopic analysis. Acridine orange staining and nuclease digestion methods have indicated that the virus is probably a ribonucleic acid virus.
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- Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Complement fixation and tissue culture assays for mouse leukemia viruses. Proc Natl Acad Sci U S A. 1965 May;53(5):931–938. doi: 10.1073/pnas.53.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
- MCCREA J. F., EPSTEIN R. S., BARRY W. H. Use of potassium tartrate for equilibrium density-gradient centrifugation of animal viruses. Nature. 1961 Jan 21;189:220–221. doi: 10.1038/189220a0. [DOI] [PubMed] [Google Scholar]
- MOLONEY J. B. The murine leukemias. Fed Proc. 1962 Jan-Feb;21:19–31. [PubMed] [Google Scholar]
- O'CONNOR T. E., RAUSCHER F. J., ZEIGEL R. F. DENSITY GRADIENT CENTRIFUGATION OF A MURINE LEUKEMIA VIRUS. Science. 1964 May 29;144(3622):1144–1147. doi: 10.1126/science.144.3622.1144. [DOI] [PubMed] [Google Scholar]
- SMITH K. O., MELNICK J. L. ADENOVIRUS-LIKE PARTICLES FROM CANCERS INDUCED BY ADENOVIRUS-12 BUT FREE OF INFECTIOUS VIRUS. Science. 1964 Sep 11;145(3637):1190–1192. doi: 10.1126/science.145.3637.1190. [DOI] [PubMed] [Google Scholar]
- STENBACK W. A., DURAND D. P. HOST INFLUENCE ON THE DENSITY OF NEWCASTLE DISEASE VIRUS (NDV). Virology. 1963 Aug;20:545–551. doi: 10.1016/0042-6822(63)90278-2. [DOI] [PubMed] [Google Scholar]
- Stenback W. A., Van Hoosier G. L., Jr, Trentin J. J. Virus particles in hamster tumors as revealed by electron microscopy. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1219–1223. doi: 10.3181/00379727-122-31365. [DOI] [PubMed] [Google Scholar]
- Thomas J. A., Delain E., Hollande E. Morphogenèse d'un virus du hamster associé à la souche BHK, ou à des tumeurs. C R Acad Sci Hebd Seances Acad Sci D. 1967 Jan 30;264(5):785–788. [PubMed] [Google Scholar]