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. 1968 Oct;2(10):1191–1199. doi: 10.1128/jvi.2.10.1191-1199.1968

Morphology of the Nucleoprotein Component of Rabies Virus

Klaus Hummeler 1,2, Natale Tomassini 1,2, Frantisek Sokol 1,2, Ernst Kuwert 1,2, Hilary Koprowski 1,2
PMCID: PMC375452  PMID: 4881422

Abstract

The intracytoplasmic ground substance, or matrix, associated with the development of rabies virus and the nucleocapsid of the virus were investigated. The filaments of the matrix were identified as virus-specific by means of ferritin-labeled antibodies. In thin sections, the diameter was 15 nm and the strands seemed to be incorporated into virions during morphogenesis of the virus. The nucleocapsid was isolated from purified virus preparations and was studied in negative contrast. The rabies nucleocapsid appeared as a single-stranded helix with a diameter of 16 nm and a periodicity of 7.5 nm; its length was in excess of 1 μm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATANASIU P., LEPINE P., DRAGONAS P. ETUDE CIN'ETIQUE DU VIRUS RABIQUE EN CULTURE DES TISSUS 'A L'AIDE DES ANTICORPS FLUORESCENTS ET DES COUPES ULTRA-FINES. Ann Inst Pasteur (Paris) 1963 Nov;105:813–824. [PubMed] [Google Scholar]
  2. ATANASIU P., ORTH G., SISMAN J., BARREAU C. IDENTIFICATION IMMUNOLOGIQUE DU VIRION RABIQUE EN CULTURES CELLULAIRES PAR LES ANTICORPS SP'ECIFIQUES CONJUGU'ES 'A LA FERRITINE. C R Hebd Seances Acad Sci. 1963 Oct 7;257:2204–2207. [PubMed] [Google Scholar]
  3. Compans R. W., Choppin P. W. Isolation and properties of the helical nucleocapsid of the parainfluenza virus SV5. Proc Natl Acad Sci U S A. 1967 Apr;57(4):949–956. doi: 10.1073/pnas.57.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Compans R. W., Holmes K. V., Dales S., Choppin P. W. An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology. 1966 Nov;30(3):411–426. doi: 10.1016/0042-6822(66)90119-x. [DOI] [PubMed] [Google Scholar]
  5. DAVIES M. C., ENGLERT M. E., SHARPLESS G. R., CABASSO V. J. THE ELECTRON MICROSCOPY OF RABIES VIRUS IN CULTURES OF CHICKEN EMBRYO TISSUES. Virology. 1963 Dec;21:642–651. doi: 10.1016/0042-6822(63)90238-1. [DOI] [PubMed] [Google Scholar]
  6. David-West T. S., Labzoffsky N. A. Electron microscopic studies on the development of vesicular stomatitis virus. Arch Gesamte Virusforsch. 1968;23(1):105–125. doi: 10.1007/BF01242119. [DOI] [PubMed] [Google Scholar]
  7. Diamond L. Two spontaneously transformed cell lines derived from the same hamster embryo culture. Int J Cancer. 1967 Mar 15;2(2):143–152. doi: 10.1002/ijc.2910020209. [DOI] [PubMed] [Google Scholar]
  8. GOLDWASSER R. A., KISSLING R. E. Fluorescent antibody staining of street and fixed rabies virus antigens. Proc Soc Exp Biol Med. 1958 Jun;98(2):219–223. doi: 10.3181/00379727-98-23996. [DOI] [PubMed] [Google Scholar]
  9. Hosaka Y., Kitano H., Ikeguchi S. Studies on the pleomorphism of HVJ virons. Virology. 1966 Jun;29(2):205–221. doi: 10.1016/0042-6822(66)90027-4. [DOI] [PubMed] [Google Scholar]
  10. Howe C., Morgan C., de Vaux St Cyr C., Hsu K. C., Rose H. M. Morphogenesis of type 2 parainfluenza virus examined by light and electron microscopy. J Virol. 1967 Feb;1(1):215–237. doi: 10.1128/jvi.1.1.215-237.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hummeler K., Koprowski H., Wiktor T. J. Structure and development of rabies virus in tissue culture. J Virol. 1967 Feb;1(1):152–170. doi: 10.1128/jvi.1.1.152-170.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JOHNSON R. T., MERCER E. H. THE DEVELOPMENT OF FIXED RABIES VIRUS IN MOUSE BRAIN. Aust J Exp Biol Med Sci. 1964 Aug;42:449–456. doi: 10.1038/icb.1964.42. [DOI] [PubMed] [Google Scholar]
  13. Kaplan M. M., Wiktor T. J., Maes R. F., Campbell J. B., Koprowski H. Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle growth curve. J Virol. 1967 Feb;1(1):145–151. doi: 10.1128/jvi.1.1.145-151.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kingsbury D. W., Darlington R. W. Isolation and properties of Newcastle disease virus nucleocapsid. J Virol. 1968 Mar;2(3):248–255. doi: 10.1128/jvi.2.3.248-255.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kissling R. E., Robinson R. Q., Murphy F. A., Whitfield S. G. Agent of disease contracted from green monkeys. Science. 1968 May 24;160(3830):888–890. doi: 10.1126/science.160.3830.888. [DOI] [PubMed] [Google Scholar]
  16. Kitajima E. W., Costa A. S. Morphology and developmental stages of Gomphrena virus. Virology. 1966 Aug;29(4):523–539. doi: 10.1016/0042-6822(66)90277-7. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  19. MATSUMOTO S. ELECTRON MICROSCOPE STUDIES OF RABIES VIRUS IN MOUSE BRAIN. J Cell Biol. 1963 Dec;19:565–591. doi: 10.1083/jcb.19.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. MATSUMOTO S. Electron microscopy of nerve cells infected with street rabies virus. Virology. 1962 May;17:198–202. doi: 10.1016/0042-6822(62)90099-5. [DOI] [PubMed] [Google Scholar]
  21. MacLeod R., Black L. M., Moyer F. H. The fine structure and intracellular localization of potato yellow dwarf virus. Virology. 1966 Aug;29(4):540–552. doi: 10.1016/0042-6822(66)90278-9. [DOI] [PubMed] [Google Scholar]
  22. McCombs R. M., Melnick M. B., Brunschwig J. P. Biophysical studies of vesicular stomatitis virus. J Bacteriol. 1966 Feb;91(2):803–812. doi: 10.1128/jb.91.2.803-812.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Miyamoto K., Matsumoto S. Comparative studies between pathogenesis of street and fixed rabies infection. J Exp Med. 1967 Mar 1;125(3):447–456. doi: 10.1084/jem.125.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Murphy F. A., Coleman P. H., Whitfield S. G. Electron microscopic observations of Flanders virus. Virology. 1966 Oct;30(2):314–317. doi: 10.1016/0042-6822(66)90107-3. [DOI] [PubMed] [Google Scholar]
  25. Siegert R., Shu H. L., Slenczka W., Peters D., Müller G. Zur Atiologie einer umbekannten, von Affen ausgegangenen menschlichen Infektionskrankheit. Dtsch Med Wochenschr. 1967 Dec 22;92(51):2341–2343. doi: 10.1055/s-0028-1106144. [DOI] [PubMed] [Google Scholar]
  26. Simpson R. W., Hauser R. E. Structural components of vesicular stomatitis virus. Virology. 1966 Aug;29(4):654–667. doi: 10.1016/0042-6822(66)90289-3. [DOI] [PubMed] [Google Scholar]
  27. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. Purification of rabies virus grown in tissue culture. J Virol. 1968 Aug;2(8):836–849. doi: 10.1128/jvi.2.8.836-849.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Zlotnik I., Simpson D. I., Howard D. M. Structure of the vervet-monkey-disease agent. Lancet. 1968 Jul 6;2(7558):26–28. doi: 10.1016/s0140-6736(68)92894-8. [DOI] [PubMed] [Google Scholar]

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