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. 1968 Nov;2(11):1290–1295. doi: 10.1128/jvi.2.11.1290-1295.1968

Replication of Coliphage M-13 I. Effects on Host Cells After Synchronized Infection1

Lyle R Brown 1,2,2, C E Dowell 1,2
PMCID: PMC375468  PMID: 4883016

Abstract

Techniques have been described for synchronization of bacteriophage M-13 infection of host cells. The latent period in infected cells was 10 min, and no appreciable number of intracellular phage was observed. Phage production proceeded in three phases after release of the starvation block: an initial rapid exponential rate of progeny phage release without cell lysis, a period of rate transition accompanying the resumption of host cell division, and a second, slower exponential rate of phage production which paralleled the rate of host cell division. The size of infected cells was not affected by infection, but the generation time was increased by 25%. Starved infected cells exhibited a much longer lag in attaining an exponential rate of growth upon the addition of nutrients than did an uninfected control culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradley D. E., Dewar C. A. Intracellular changes in cells of Escherichia coli infected with a filamentous bacteriophage. J Gen Virol. 1967 Apr;1(2):179–188. doi: 10.1099/0022-1317-1-2-179. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
  3. Dowell C. E., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. IX. Studies on the physiology of three phi-X174 temperature-sensitive mutants. J Mol Biol. 1966 Apr;16(2):374–386. doi: 10.1016/s0022-2836(66)80180-8. [DOI] [PubMed] [Google Scholar]
  4. GAREN A., SIDDIQI O. Suppression of mutations in the alkaline phosphatase structural cistron of E. coli. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1121–1127. doi: 10.1073/pnas.48.7.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOFFMANN BERLING H., MAZE R. RELEASE OF MALE-SPECIFIC BACTERIOPHAGES FROM SURVIVING HOST BACTERIA. Virology. 1964 Mar;22:305–313. doi: 10.1016/0042-6822(64)90021-2. [DOI] [PubMed] [Google Scholar]
  6. HOFFMANN-BERLING H., DUERWALD H., BEULKE I. EIN FAEDIGER DNS-PHAGE (FD) UND EIN SPHAERISCHER RNS-PHAGE (FR) WIRTSSPEZIFISCH FUER MAENNLICHE STAEMME VON E. COLI. III. BIOLOGISCHES VERHALTEN VON FD UND FR. Z Naturforsch B. 1963 Nov;18:893–898. [PubMed] [Google Scholar]
  7. Harvey R. J., Marr A. G. Measurement of size distributions of bacterial cells. J Bacteriol. 1966 Oct;92(4):805–811. doi: 10.1128/jb.92.4.805-811.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Minamishima Y., Takeya K., Ohnishi Y., Amako K. Physicochemical and biological properties of fibrous Pseudomonas bacteriophages. J Virol. 1968 Mar;2(3):208–213. doi: 10.1128/jvi.2.3.208-213.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Panter R. A., Symons R. H. Isolation and properties of a DNA-containing rod-shaped bacteriophage. Aust J Biol Sci. 1966 Aug;19(4):565–573. doi: 10.1071/bi9660565. [DOI] [PubMed] [Google Scholar]
  10. SALIVAR W. O., TZAGOLOFF H., PRATT D. SOME PHYSICAL-CHEMICAL AND BIOLOGICAL PROPERTIES OF THE ROD-SHAPED COLIPHAGE M13. Virology. 1964 Nov;24:359–371. doi: 10.1016/0042-6822(64)90173-4. [DOI] [PubMed] [Google Scholar]
  11. Schwartz F. M., Zinder N. D. Morphological changes in Escherichia coli infected with the DNA bacteriophage fl. Virology. 1968 Feb;34(2):352–355. doi: 10.1016/0042-6822(68)90246-8. [DOI] [PubMed] [Google Scholar]
  12. TZAGOLOFF H., PRATT D. THE INITIAL STEPS IN INFECTION WITH COLIPHAGE M13. Virology. 1964 Nov;24:372–380. doi: 10.1016/0042-6822(64)90174-6. [DOI] [PubMed] [Google Scholar]
  13. Williams P. G., Fenwick M. L. Degradation of the filamentous phage ZJ-2 by sodium dodecylsulphate. Nature. 1967 May 13;214(5089):712–713. doi: 10.1038/214712a0. [DOI] [PubMed] [Google Scholar]

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