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. 1968 Nov;2(11):1296–1307. doi: 10.1128/jvi.2.11.1296-1307.1968

Replication of Coliphage M-13 II. Intracellular Deoxyribonucleic Acid Forms Associated with M-13 Infection of Mitomycin C-treated Cells1

Lyle R Brown 1,2,2, C E Dowell 1,2
PMCID: PMC375469  PMID: 4883017

Abstract

Intracellular deoxyribonucleic acid (DNA) forms associated with bacteriophage M-13 infection have been isolated and characterized. Escherichia coli HF4704 (F+, hcr−, thy−) cells were treated with mitomycin C to inhibit host-cell DNA synthesis and were then infected with phage M-13. This treatment permitted radioactive labeling of phage-specific DNA forms with 3H-thymine. These labeled DNA components were characterized by sucrose density sedimentation and equilibrium density gradient centrifugation in neutral and ethidium bromide CsCl gradient. Two double-stranded circular forms were found with properties analogous to the replicative form I and replicative form II of φX174. A third component, identified as single-stranded DNA, was isolated in some samples removed 45 min after phage synthesis was initiated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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