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. 1968 Nov;2(11):1315–1325. doi: 10.1128/jvi.2.11.1315-1325.1968

Electron Microscopic Observations of Mouse Brain Infected with Bunyamwera Group Arboviruses

Frederick A Murphy 1, Alyne K Harrison 1, Theodore Tzianabos 1,1
PMCID: PMC375471  PMID: 5701826

Abstract

Ultrathin sections of brain tissue from mice infected with three Bunyamwera group arboviruses, Bunyamwera, Tensaw, and Maguari, were examined by electron microscopy. In all three cases, virus was observed budding from membranes into intracytoplasmic cisternae. Mature virus was found accumulated within these distended cisternae and in extracellular spaces. The three viruses were morphologically indistinguishable; particles were round or oval, 98 nm in diameter, and had a closely adherent irregular envelope.

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Selected References

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  1. Acheson N. H., Tamm I. Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967 May;32(1):128–143. doi: 10.1016/0042-6822(67)90261-9. [DOI] [PubMed] [Google Scholar]
  2. Becker W. B., McIntosh K., Dees J. H., Chanock R. M. Morphogenesis of avian infectious bronchitis virus and a related human virus (strain 229E). J Virol. 1967 Oct;1(5):1019–1027. doi: 10.1128/jvi.1.5.1019-1027.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CASALS J., WHITMAN L. A new antigenic group of arthropod-borne viruses: the Bunyamwera group. Am J Trop Med Hyg. 1960 Jan;9:73–77. doi: 10.4269/ajtmh.1960.9.73. [DOI] [PubMed] [Google Scholar]
  4. CAUSEY O. R., CAUSEY C. E., MAROJA O. M., MACEDO D. G. The isolation of arthropod-borne viruses, including members of two hitherto undescribed serological groups, in the Amazon region of Brazil. Am J Trop Med Hyg. 1961 Mar;10:227–249. doi: 10.4269/ajtmh.1961.10.227. [DOI] [PubMed] [Google Scholar]
  5. DAVID-FERREIRA J. F., MANAKER R. A. AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS. J Cell Biol. 1965 Jan;24:57–78. doi: 10.1083/jcb.24.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Filshie B. K., Rehacek J. Studies of the morphology of Murray Valley encephalitis and Japanese encephalitis viruses growing in cultured mosquito cells. Virology. 1968 Mar;34(3):435–443. doi: 10.1016/0042-6822(68)90063-9. [DOI] [PubMed] [Google Scholar]
  7. Holmes I. H., Wark M. C., Jack I., Grutzner J. Identification of two possible types of virus particle in rubella-infected cells. J Gen Virol. 1968 Jan;2(1):37–42. doi: 10.1099/0022-1317-2-1-37. [DOI] [PubMed] [Google Scholar]
  8. Holms I. H., Warburton M. F. Is rubella an arbovirus? Lancet. 1967 Dec 9;2(7528):1233–1236. doi: 10.1016/s0140-6736(67)90568-5. [DOI] [PubMed] [Google Scholar]
  9. KOKERNOT R. H., SMITHBURN K. C., DE MEILLON B., PATERSON H. E. Isolation of Bunyamwera virus from a naturally infected human being and further isolations from Aedes (Banksinella) circumluteolus theo. Am J Trop Med Hyg. 1958 Nov;7(6):579–584. doi: 10.4269/ajtmh.1958.7.579. [DOI] [PubMed] [Google Scholar]
  10. MOLLENHAUER H. H. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY. Stain Technol. 1964 Mar;39:111–114. [PubMed] [Google Scholar]
  11. MORGAN C., HOWE C., ROSE H. M. Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J Exp Med. 1961 Jan 1;113:219–234. doi: 10.1084/jem.113.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MUSSGAY M. GROWTH CYCLE OF ARBOVIRUSES IN VERTEBRATE AND ARTHROPOD CELLS. Prog Med Virol. 1964;6:193–267. [PubMed] [Google Scholar]
  13. Murphy F. A., Coleman P. H. California group arboviruses: immunodiffusion studies. J Immunol. 1967 Aug;99(2):276–284. [PubMed] [Google Scholar]
  14. Murphy F. A., Halonen P. E., Harrison A. K. Electron microscopy of the development of rubella virus in BHK-21 cells. J Virol. 1968 Oct;2(10):1223–1227. doi: 10.1128/jvi.2.10.1223-1227.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murphy F. A., Whitfield S. G., Coleman P. H., Calisher C. H., Rabin E. R., Jenson A. B., Melnick J. L., Edwards M. R., Whitney E. California group arboviruses: electron microscopic studies. Exp Mol Pathol. 1968 Aug;9(1):44–56. doi: 10.1016/0014-4800(68)90049-x. [DOI] [PubMed] [Google Scholar]
  16. OTA Z. ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS VIRUS IN PORCINE KIDNEY STABLE (PS) CELLS. Virology. 1965 Mar;25:372–378. doi: 10.1016/0042-6822(65)90057-7. [DOI] [PubMed] [Google Scholar]
  17. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SMITHBURN K. C., BUGHER J. C. Ultrafiltration of recently isolated neurotropic viruses. J Bacteriol. 1953 Aug;66(2):173–177. doi: 10.1128/jb.66.2.173-177.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SOUTHAM C. M., MOORE A. E. West Nile, Ilheus, and Bunyamwera virus infections in man. Am J Trop Med Hyg. 1951 Nov;31(6):724–741. doi: 10.4269/ajtmh.1951.s1-31.724. [DOI] [PubMed] [Google Scholar]
  20. SOUTHAM C. M., SHIPKEY F. H., BABCOCK V. I., BAILEY R., ERLANDSON R. A. VIRUS BIOGRAPHIES. I. GROWTH OF WEST NILE AND GUAROA VIRUSES IN TISSUE CULTURE. J Bacteriol. 1964 Jul;88:187–199. doi: 10.1128/jb.88.1.187-199.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Saikku P., von Bonsdorff C. H. Electron microscopy of the Uukuniemi virus, an ungrouped arbovirus. Virology. 1968 Apr;34(4):804–806. doi: 10.1016/0042-6822(68)90104-9. [DOI] [PubMed] [Google Scholar]
  22. Tauraso N. M., Shelokov A. Arboviruses--a problem in classification. Arch Gesamte Virusforsch. 1967;22(1):273–279. doi: 10.1007/BF01240522. [DOI] [PubMed] [Google Scholar]
  23. YASUZUMI G., TSUBO I. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VIRUS. II. ELECTRON MICROSCOPE STUDIES OF NEURONS INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1965 Apr;12:304–316. doi: 10.1016/s0022-5320(65)80101-0. [DOI] [PubMed] [Google Scholar]
  24. YASUZUMI G., TSUBO I., SUGIHARA R., NAKAI Y. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VISUS. I. ELECTRON MICROSCOPE STUDIES OF MICROGLIA INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1964 Oct;11:213–229. doi: 10.1016/s0022-5320(64)90029-2. [DOI] [PubMed] [Google Scholar]

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